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Plant and Soil

, Volume 370, Issue 1–2, pp 47–57 | Cite as

Hydrogen-rich water alleviates salt stress in rice during seed germination

  • Sheng Xu
  • Susong Zhu
  • Yilong Jiang
  • Ning Wang
  • Ren Wang
  • Wenbiao Shen
  • Jie Yang
Regular Article

Abstract

Aims

This study investigated the molecular mechanism of hydrogen-rich water (HRW)-mediated enhancement of tolerance against salinity stress during rice seed germination.

Methods

A combination of physiological and molecular approaches was used to study the effect of HRW on the alleviation of salinity stress.

Results

A 100-mM NaCl stress caused the increase of H2 release in germinating rice seeds. With respect to samples treated with 100 mM NaCl alone, exogenous HRW pretreatments differentially attenuated the inhibition of seed germination and seedling growth caused by salinity. Further results showed that both 50 % (in particular) and 100 % concentration of HRW could activate α/β-amylase activity, thus accelerating the formation of reducing sugar and total soluble sugar. HRW also enhanced total, isozymatic activities or corresponding transcripts of antioxidant enzymes, including superoxide dismutase, catalase, and ascorbate peroxidase. These results were confirmed by the alleviation of oxidative damage, as indicated by a decrease of thiobarbituric acid reactive substances. Additionally, the ratio of potassium (K) to sodium (Na) in both the shoot and root parts was increased.

Conclusions

Together, our results suggested that exogenous HRW treatment on rice seeds may be a good option to alleviate salinity stress.

Keywords

Hydrogen gas Ion homeostasis Oxidative damage Salinity tolerance Seed germination Oryza sativa 

Abbreviations

APX

Ascorbate peroxidase

CAT

Catalase

DHAR

Dehydroascorbate reductase

GR

Glutathione reductase

HRW

Hydrogen-rich water

MDHAR

Monodehydroascorbate reductase

ROS

Reactive oxygen species

SOD

Superoxide dismutase

TBARS

Thiobarbituric acid reactive substances

Notes

Acknowledgments

This work was supported by the Key Science and Technology Specific Projects of Guizhou Province (grant no. 2012-6005), the Rice Breeding, Cultivation and Industrialization Program of Guizhou Province (grant no. 20114003), and the Jiangsu Agricultural Science and Technology Independence Innovation Project (grant no. CX(11)4022).

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.College of Life SciencesNanjing Agricultural UniversityNanjingChina
  2. 2.Institute of Botany, Jiangsu Province and the Chinese Academy of SciencesJiangsu Province Key Laboratory for Plant Ex-situ ConservationNanjingChina
  3. 3.Guizhou Rice Research InstituteGuiyangChina
  4. 4.Institute of Food CropsJiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R&D CenterNanjingChina

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