Plant and Soil

, Volume 367, Issue 1–2, pp 363–377 | Cite as

Phenotypic and genotypic characterisation of root nodule bacteria nodulating Millettia pinnata (L.) Panigrahi, a biodiesel tree

  • Ni Luh Arpiwi
  • Guijun Yan
  • Elizabeth L. Barbour
  • Julie A. Plummer
  • Elizabeth Watkin
Regular Article
First Online:
Received:
Accepted:

Abstract

Aims

Milletia pinnata is a leguminous tropical tree that produces seed oil suitable for biodiesel and is targeted to be planted on marginal land associated with nitrogen poor soil. This study aimed to identify effective rhizobia species for M. pinnata.

Methods

Soil samples were collected from M. pinnata grown in Kununurra, Australia. Rhizobia were trapped, characterised and sequenced for 16S rRNA, atpD, dnaK and recA genes.

Results

Forty isolates tolerated pH 7 – 9, temperatures 29 – 37 °C, salinity below 1 % NaCl, and had optimal growth on mannitol, arabinose or glutamate as a single carbon source, a few grew on sucrose and none grew on lactose. Inoculation of isolates increased shoot dry weight of M. pinnata’s seedlings in nitrogen minus media. Slow-growing isolates were closely related to Bradyrhizobium yuanmingense, Bradyrhizobium sp. DOA10, Bradyrhizobium sp. ORS305 and B. liaoningense LMG 18230T. The fast-growing isolates related to Rhizobium sp. 8211, R. miluonense CCBAU 41251T, R miluonense CC-B-L1, Rhizobium sp. CCBAU 51330 and Rhizobium sp. 43015

Conclusions

Millettia pinnata was effectively nodulated by slow-growing isolates related to Bradyrhizobium yuanmingense, Bradyrhizobium sp. DOA10 Bradyrhizobium sp. ORS305, B. liaoningense LMG 18230T and fast-growing isolates related Rhizobium sp. 8211, R. miluonense, Rhizobium sp. CCBAU 51330 and Rhizobium sp. 43015

Keywords

Bradyrhizobium Housekeeping genes Pongamia Rhizobia Rhizobium 16S rRNA gene 
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Notes

Acknowledgements

We would like to thank the following organizations and people: Department of Higher Education, Republic of Indonesia (for the PhD scholarship for NLA), Forest Products Commission of Western Australia, the Centre for Legumes in Mediterranean Agriculture and the School of Plant Biology at the University of Western Australia for funding the research. Thanks to Len J Norris for field assistance and set up for the rhizobia trapping experiment and to Ms Frances Brigg at SABC, Murdoch University for help in sequencing. We also thank Dr Jason Terpolillli from Centre for Rhizobium Studies, Murdoch University and Associate Professor Susan Barker from School of Plant Biology the University of Western Australia for their advice on the manuscript.

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Ni Luh Arpiwi
    • 1
    • 3
  • Guijun Yan
    • 1
    • 2
  • Elizabeth L. Barbour
    • 1
    • 4
  • Julie A. Plummer
    • 1
    • 2
  • Elizabeth Watkin
    • 5
  1. 1.School of Plant Biology, Faculty of Natural and Agricultural SciencesUniversity of Western AustraliaCrawleyAustralia
  2. 2.Institute of AgricultureUniversity of Western AustraliaCrawleyAustralia
  3. 3.Biology Department, Faculty of Natural and Basic SciencesUdayana UniversityBaliIndonesia
  4. 4.Forest Products Commission of Western AustraliaRivervaleAustralia
  5. 5.School of Biomedical Science, Faculty of Health SciencesCurtin UniversityBentleyAustralia

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