Energy-dispersive X-ray fluorescence spectrometry as a tool for zinc, iron and selenium analysis in whole grain wheat
- 935 Downloads
Background and aims
Crop biofortification programs require fast, accurate and inexpensive methods of identifying nutrient dense genotypes. This study investigated energy-dispersive X-ray fluorescence spectrometry (EDXRF) for the measurement of zinc (Zn), iron (Fe) and selenium (Se) concentrations in whole grain wheat.
Grain samples were obtained from existing biofortification programs. Reference Zn, Fe and Se concentrations were obtained using inductively coupled plasma optical emission spectrometry (ICP-OES) and/or inductively coupled plasma mass spectrometry (ICP-MS). One set of 25 samples was used to calibrate for Zn (19–60 mg kg–1) and Fe (26–41 mg kg–1), with 25 further samples used to calibrate for Se (2–31 mg kg–1 ). Calibrations were validated using an additional 40–50 wheat samples.
EDXRF limits of quantification (LOQ) were estimated as 7, 3 and 2 mg kg–1 for Zn, Fe, and Se, respectively. EDXRF results were highly correlated with ICP-OES or -MS values. Standard errors of EDXRF predictions were ±2.2 mg Zn kg–1, ±2.6 mg Fe kg–1, and ±1.5 mg Se kg–1.
EDXRF offers a fast and economical method for the assessment of Zn, Fe and Se concentration in wheat biofortification programs.
KeywordsXRF EDXRF Biofortification Micronutrient Plant
This work was funded by HarvestPlus. We thank Oxford Instruments, Caroline Suster and Neal Robson for help identifying optimal Zn and Fe EDXRF conditions, Waite Analytical Services for ICP-OES and -MS analysis, and Wendy Telfer and Nicholas Collins for helpful suggestions on the manuscript.
- Hart DJ, Fairweather-Tait SJ, Broadley MR, Dickinson SJ, Foot I, Knott P, McGrath SP, Mowat H, Norman K, Scott PR, Stroud JL, Tucker M, White PJ, Zhao FJ, Hurst R (2011) Selenium concentration and speciation in biofortified flour and bread: Retention of selenium during grain biofortification, processing and production of Se-enriched food. Food Chem 126:1771–1778CrossRefGoogle Scholar
- Paltridge NG, Palmer LJ, Milham PJ, Guild GE, Stangoulis JCR (2012) Energy-dispersive X-ray fluorescence analysis of zinc and iron concentration in rice and pearl millet grain. Plant Soil (in press) doi: 10.1007/s11104–011–1104–4]
- West M, Ellis AT, Potts PJ, Streli C, Vanhoof C, Wegrzynek D, Wobrauschek P (2009) Atomic spectrometry update. X-Ray fluorescence spectrometry. J Anal At Spectrom 24(1289–1):326Google Scholar
- West M, Ellis AT, Potts PJ, Streli C, Vanhoof C, Wegrzynek D, Wobrauschek P (2010) Atomic spectrometry update–X-Ray fluorescence spectrometry. J Anal At Spectrom 25(1503–1):545Google Scholar