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Plant and Soil

, Volume 359, Issue 1–2, pp 297–319 | Cite as

Microarray analysis of humic acid effects on Brassica napus growth: Involvement of N, C and S metabolisms

  • Laëtitia JanninEmail author
  • Mustapha Arkoun
  • Alain Ourry
  • Philippe Laîné
  • Didier Goux
  • Maria Garnica
  • Marta Fuentes
  • Sara San Francisco
  • Roberto Baigorri
  • Florence Cruz
  • Fabrice Houdusse
  • José-Maria Garcia-Mina
  • Jean-Claude Yvin
  • Philippe Etienne
Regular Article

Abstract

Background & aims

Winter rapeseed (Brassica napus) is characterized by a low N recovery in seeds and requires high rates of fertilization to maintain yield. Its nutrient use efficiency could be improved by addition of a biostimulant such as humic acids whose physiological effects have been described previously in some plant species. However, to our knowledge, no study has focused on transcriptomic analyses to determine metabolic targets of this extract.

Methods

A preliminary screening of ten humic acids revealed a significant effect of one of them (HA7) on rapeseed root growth. Microarray analysis was then used on HA7-treated or non-treated plants to characterize changes in gene expression that were further supported by physiological evidence.

Results

Stimulation of nitrogen uptake (+15% in shoots and +108% in roots) and assimilation was found to be increased in a similar manner to growth while sulfate content (+76% in shoots and +137% in roots) was more strongly stimulated leading to higher sulfate accumulation. In parallel, microscopic analysis showed an enhancement of chloroplast number per cell.

Conclusion

It is therefore suggested that HA7, which promotes plant growth and nutrient uptake, could be used as a supplementary tool to improve rapeseed nitrogen use efficiency.

Keywords

Brassica napus Humic acid Microarray Growth promotion Nutrient uptake Chloroplast 

Notes

Acknowledgements

This study was a part of AZOSTIMER project selected and supported by the Pôle de compétitivité Mer-Bretagne and funded by the French FUI (Fond Unique Interministériel), Brittany Region and Saint-Malo Agglomeration. We thank Marie-Paule Bataillé and Raphaël Ségura for IRMS analyses. We acknowledge Patrick Beauclair for LICOR measurements, Julie Levallois for technical assistance in RNA extractions and q-PCR analyses, Xavier Sarda and Anne-Françoise Ameline for helping with plant culture and harvest and finally Nicolas Elie from GRECAN (Groupe Régional d’Etude sur le CANcer, Histo-imagerie quantitative, Caen, France) for microscopic analysis. We thank Laurence Cantrill for improving the English of the manuscript.

Supplementary material

11104_2012_1191_MOESM1_ESM.xls (296 kb)
Supplemental Table S1 List of the differentially expressed genes in shoots and roots of rapeseed after 3 days of HA7 supply to the roots. (XLS 295 kb)
11104_2012_1191_MOESM2_ESM.xls (35 kb)
Supplemental Table S2 List of the differentially expressed genes in shoots and roots of rapeseed after 30 days of HA7 supply to the roots. (XLS 35 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Laëtitia Jannin
    • 1
    • 2
    Email author
  • Mustapha Arkoun
    • 1
    • 2
  • Alain Ourry
    • 1
    • 2
  • Philippe Laîné
    • 1
    • 2
  • Didier Goux
    • 3
  • Maria Garnica
    • 4
  • Marta Fuentes
    • 4
  • Sara San Francisco
    • 4
  • Roberto Baigorri
    • 4
  • Florence Cruz
    • 5
  • Fabrice Houdusse
    • 5
  • José-Maria Garcia-Mina
    • 4
  • Jean-Claude Yvin
    • 5
  • Philippe Etienne
    • 1
    • 2
  1. 1.Université de Caen Basse-Normandie, UMR 950 Ecophysiologie VégétaleCaen CedexFrance
  2. 2.INRA, UMR 950 Ecophysiologie VégétaleCaen CedexFrance
  3. 3.Université de Caen Basse-Normandie, Centre de Microscopie Appliquée à la Biologie (CMABio)Caen CedexFrance
  4. 4.TIMAC Agro SpainOrcoyenSpain
  5. 5.Centre de Recherche International en Agroscience, CRIAS-TAI, Groupe RoullierDinardFrance

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