Succession of root-associated fungi in Pisum sativum during a plant growth cycle as examined by 454 pyrosequencing
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Roots are inhabited by a broad range of fungi, including pathogens and mycorrhizal fungi, with functional traits related to plant health and nutrition. Management of these fungi in agroecosystems requires profound knowledge about their ecology. The main objective of this study was to examine succession patterns of root-associated fungi in pea during a full plant growth cycle.
Plants were grown in pots with field soil in a growth chamber under controlled conditions. Fungal communities in pea roots were analyzed at different plant growth stages including the vegetative growth, flowering and senescence, using 454 pyrosequencing.
One hundred and twenty one non-singleton operational taxonomic units (OTUs) representing fungal species were detected. Pathogenic and arbuscular mycorrhizal fungi dominated during the vegetative growth stage, whereas saprotrophic fungi dominated during plant senescence.
In conclusion, the results from the present study demonstrated highly diverse fungal communities in pea roots with clear succession patterns related to fungal traits.
Keywordsfungal diversity arbuscular mycorrhizal fungi pathogens Pisum sativum
We thank Karsten Malmskov (Ardo A/S) and Steen Meier for help sampling soil in the field, Anne-Pia Larsen and Jacob I. Bañuelos Trejo for assistance with root staining, Henriette Nyskjold for technical assistance, Bernd Wollenweber for critical review on the manuscript and Kirsten Jensen for linguistic editing.
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