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Plant and Soil

, Volume 357, Issue 1–2, pp 395–405 | Cite as

Molecular characterization of root-associated fungal communities in relation to health status of Pisum sativum using barcoded pyrosequencing

  • L. Yu
  • M. Nicolaisen
  • J. Larsen
  • S. RavnskovEmail author
Regular Article

Abstract

Background and Aims

Root-associated fungi are known to be important for plant health and nutrition, but only few studies have addressed their diversity in relation to plant health status.

Methods

Fungal diversity in roots of healthy and diseased Pisum sativum plants was examined in terms of barcoded pyrosequencing of the nuclear ribosomal internal transcribed spacer 1. The CLOTU program was used for filtering and clustering of sequences, and Chao 1 estimator was used to calculate fungal richness.

Results

Principal component analyses (PCA) showed that the structure of root-associated fungal communities differed between sites and a clear relationship between root-associated fungal communities and plant health status was found. For example, the arbuscular mycorrhizal (AM) fungus Glomus caledonium was prevalent in roots of healthy plants, whereas the pathogenic fungus Phoma sojicola was prevalent in roots of diseased plants.

Conclusions

The present study revealed clear differences in composition of root-associated fungi from four field sites, and correlations between abundance of several root-associated fungi and plant health status were found.

Keywords

Fungal community Plant health AM fungi Root pathogens Pisum sativum 

Notes

Acknowledgements

We thank Karsten Malmskov (Ardo A/S), Lihui Xu and Steen Meier for support with field soil sampling and Anne-Pia Larsen and Henriette Nyskjold for their excellent technical laboratory assistance, and Annemarie Fejer Justesen for critical review of the manuscript.

Supplementary material

11104_2012_1180_MOESM1_ESM.docx (34 kb)
Table 1 Supplementary Material_ The list of all 142 OTUs (DOCX 34 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Faculty of Science and Technology, Department of AgroecologyAarhus UniversitySlagelseDenmark
  2. 2.Universidad Nacional Autónoma de México, Centro de Investigaciones en EcosistemasMoreliaMexico

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