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Plant and Soil

, Volume 353, Issue 1–2, pp 19–31 | Cite as

Short-term impacts of soil freeze-thaw cycles on roots and root-associated fungi of Holcus lanatus and Calluna vulgaris

  • Juergen KreylingEmail author
  • Derek Peršoh
  • Sebastian Werner
  • Meike Benzenberg
  • Jens Wöllecke
Regular Article

Abstract

Background and aims

Soil freeze-thaw cycle (FTC) regimes are altered by climate change and known to influence nutrient cycling and plant growth. Here, we explore mechanistic explanations for the changing plant performance of the grass Holcus lanatus and the dwarf shrub Calluna vulgaris.

Methods

144 plant-soil mesocosms were subjected to different FTC-regimes in a climate chamber. Root injury, fungal activity and fungal composition (ITS-sequencing) were quantified.

Results

The applied FTC-scenarios increased root injury by 23% on average while no strong differences between scenarios was found. Root damage was greater in C. vulgaris than in H. lanatus. Fungal activity increased due to the FTC-manipulation and was higher in the C. vulgaris samples, although activity was generally low. No significant shift in the fungal community composition was found immediately after the applied FTCs. A saprobic (Aureobasidium pullulans) and a potentially mycorrhizal fungus (Sebacinales) showed opposing responses to the FTC-manipulation in the different host plants, while a potential phytopathogen (Callophora) decreased in frequency.

Conclusions

Increased fungal activity within these samples is suggested to be related to an increased dominance of saprobic taxa, but not to a shift in qualitative community composition. Single pathogenic species entering the plants through the observed root injuries subsequent to FTC treatments however, may alter plant performance. While these results suggest the importance of root injury for the response of vegetation to FTCs, fungal activity and pathogenic infection need to be further studied under field conditions over longer time periods.

Keywords

Winter ecology Frost Freezing-thawing RFLP Temperature tolerance Fungal community ITS rRNA gene Clone libraries 

Notes

Acknowledgements

This study was supported by the German Science Foundation (DFG: KR 3309/3-1). We thank Katrin Kluge for hyphae quantifications, Dr. Alfons Weig for advice on molecular analyses, Sarah Gwillym-Margianto for proofreading the manuscript in terms of language and style and two anonymous reviewers for constructive comments on an earlier version of this manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Juergen Kreyling
    • 1
    Email author
  • Derek Peršoh
    • 2
  • Sebastian Werner
    • 2
  • Meike Benzenberg
    • 3
  • Jens Wöllecke
    • 4
  1. 1.BiogeographyUniversity of BayreuthBayreuthGermany
  2. 2.MycologyUniversity of BayreuthBayreuthGermany
  3. 3.Grassland Science and Renewable Plant ResourcesUniversity of KasselWitzenhausenGermany
  4. 4.Soil protection and recultivation, BTU CottbusCottbusGermany

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