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Plant and Soil

, Volume 356, Issue 1–2, pp 151–163 | Cite as

Variation of secondary metabolite levels in maize seedling roots induced by inoculation with Azospirillum, Pseudomonas and Glomus consortium under field conditions

  • Vincent Walker
  • Olivier Couillerot
  • Andreas Von Felten
  • Floriant Bellvert
  • Jan Jansa
  • Monika Maurhofer
  • René Bally
  • Yvan Moënne-Loccoz
  • Gilles ComteEmail author
Regular Article

Abstract

Background and aims

Many plant-beneficial microorganisms can influence secondary plant metabolism, but whether these effects add up when plants are co-inoculated is unclear. This issue was assessed, under field conditions, by comparing the early impacts of seed inoculation on secondary metabolite profiles of maize at current or reduced mineral fertilization levels.

Methods

Maize seeds were inoculated singly with selected strains from bacterial genera Pseudomonas and Azospirillum or mycorrhizal genus Glomus, or with these strains combined two by two or all three together. At 16 days, maize root methanolic extracts were analyzed by RP-HPLC and secondary metabolites (phenolics, flavonoids, xanthones, benzoxazionoids, etc.) identified by LC/MS.

Results

Inoculation did not impact on plant biomass but resulted in enhanced total root surface, total root volume and/or root number in certain inoculated treatments, at reduced fertilization. Inoculation led to qualitative and quantitative modifications of root secondary metabolites, particularly benzoxazinoids and diethylphthalate. These modifications depended on fertilization level and microorganism(s) inoculated. The three selected strains gave distinct results when used alone, but unexpectedly all microbial consortia gave somewhat similar results.

Conclusions

The early effects on maize secondary metabolism were not additive, as combining strains gave effects similar to those of Glomus alone. This is the first study demonstrating and analyzing inoculation effects on crop secondary metabolites in the field.

Keywords

Secondary metabolites Benzoxazinoids Diethylphtalate Mineral fertilization Zea mays L. 

Notes

Acknowledgments

This work was supported in part by the European Union (FW6 STREP project MicroMaize 036314). We are grateful to Pierre Castillon (Arvalis, Bazièges, France) and Arvalis staff at the Pouzol Etoile experimental station for implementation of the field trial. We thank Bachar Blal (Agrauxine, Quimper, France) and Aleš Látr (Symbio-M, Lanškroun, Czech Republic) for providing formulated microbial inoculants and MPN data, and Geneviève Défago (ETH Zürich) for discussions. This work made use of the platform DTAMB (IFR 41) in Université Lyon 1.

Supplementary material

11104_2011_960_MOESM1_ESM.doc (248 kb)
Fig. S1 Effect of maize inoculation treatments (indicated below the x-axis) on the ratio between dry total methanolic extract and dry root biomass at 16 days after inoculation. Different letters represent statistical differences between treatments (ANOVA and Tukey’s tests, P < 0.05) (DOC 247 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Vincent Walker
    • 1
    • 2
    • 3
  • Olivier Couillerot
    • 1
    • 2
    • 3
  • Andreas Von Felten
    • 4
  • Floriant Bellvert
    • 1
    • 2
    • 3
  • Jan Jansa
    • 5
  • Monika Maurhofer
    • 4
  • René Bally
    • 1
    • 2
    • 3
  • Yvan Moënne-Loccoz
    • 1
    • 2
    • 3
  • Gilles Comte
    • 1
    • 2
    • 3
    Email author
  1. 1.Université de LyonLyonFrance
  2. 2.Université Lyon 1VilleurbanneFrance
  3. 3.CNRS, UMR5557, Ecologie MicrobienneVilleurbanneFrance
  4. 4.Institute of Integrative BiologyETHZürichSwitzerland
  5. 5.Institute of Plant Agricultural SciencesETHLindauSwitzerland

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