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Plant and Soil

, 347:387 | Cite as

Alleviation of the adverse effects of salinity stress in wheat (Triticum aestivum L.) by seed biopriming with salinity tolerant isolates of Trichoderma harzianum

  • Laxmi Rawat
  • Y. Singh
  • N. Shukla
  • J. Kumar
Regular Article

Abstract

Salt stress is one of the major abiotic stresses limiting crop growth and productivity. This work investigated the potential of five ST isolates of Trichoderma harzianum (Th-13, Th-14, Th-19, Th-33 and Th-50) applied through seed biopriming in reducing the detrimental effects of salinity stress on wheat (Triticum aestivum L.). Growth, physiological and biochemical parameters were studied to characterize salt tolerance. One factor was treatments (T1, T2, T3, T4, T5 and T6) and second factor was four levels of salt stress viz., 0, 2, 4 and 6 dsm−1. In germination test, most of the isolates (Th-14, Th-19 and Th-13) were effective in improving germination percentage and reducing RPG during salinity stress. Seedlings raised from ST Trichoderma isolates had significantly higher root and shoot lengths, CC and MSI than control at all stress levels. The treatments Th-14, Th-19 and Th-13 showed lower accumulation of MDA content whereas proline content and phenolics were higher in treated plants under both non-saline and saline conditions. Highest MDA content was observed in control at salt stress level of 6 dSm−1. It is concluded that seed biopriming with different salinity tolerant isolates of Trichoderma reduced severity of the effects of salinity though the amelioration was better in Th-14 under present experimental material and conditions.

Keywords

Salinity Seed biopriming Trichoderma Wheat 

Abbreviations

ST

Salinity tolerant

RPG

Reduction percentage of germination

CC

Chlorophyll content

MSI

Membrane stability index

MDA

Malondialdehyde

Notes

Acknowledgements

Thanks are due to P.C. Srivastava and N.W. Zaidi for their suggestions in the conduct of the study.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Center of Advance Faculty Training, Department of Plant Pathology, College of AgricultureG.B. Pant University of Agriculture & TechnologyPantnagarIndia

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