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Plant and Soil

, 347:221 | Cite as

Glyphosate tolerance by Clitoria ternatea and Neonotonia wightii plants involves differential absorption and translocation of the herbicide

  • Hugo Cruz-HipolitoEmail author
  • Antonia Rojano-Delgado
  • José A. Domínguez-Valenzuela
  • Antonio Heredia
  • María Dolores Luque de Castro
  • Rafael De Prado
Regular Article

Abstract

Glyphosate tolerance by Clitoria ternatea, Neonotonia wightii and Amaranthus hybridus was studied in whole plants from Mexico. Experiments in a controlled growth chamber showed both legumes to be highly tolerant of glyphosate, with and ED50 values of 600.18 g ae ha–1 for C. ternatea and 362.94 g ae ha–1 for N. wightii. On the other hand, A. hybridus was highly susceptible to the herbicide (ED50 = 42.22 g ae ha–1). Shikimate accumulation peaked 96 h after treatment in the tolerant plants and the susceptible weed under 500 g ae ha–1 glyphosate. The shikimic acid content of whole leaves was 4.0 and 5.0 times higher in the susceptible weed than in N. wightii and C. ternatea, respectively. 14C-glyphosate absorption and translocation tests showed A. hybridus to absorb 30% more herbicide than the legumes 24 h after glyphosate foliar application. 14C-glyphosate translocation as measured by quantified autoradiography revealed increased translocation of the herbicide to untreated leaves and roots in A. hybridus relative to the two legumes. The cuticular surface of A. hybridus exhibited very low wax coverage relative to the epicuticular surface of N. wightii and, especially, C. ternatea. No significant degradation of glyphosate to aminomethylphosphonic acid and glyoxylate metabolites was detected among the tolerant leguminous plants or the susceptible weed population. These results indicate that the high glyphosate tolerance of Clitoria ternatea and Neonotonia wightii is mainly a result of poor penetration and translocation of the herbicide to apical growing points in their plants.

Keywords

Tolerance Glyphosate Shikimic acid Cover crops 

Notes

Acknowledgements

The authors thank the technical help of Rafael A. Roldán-Gómez. Also are grateful to Spain’s Ministry of Science and Innovation (MICINN) for funding this work through Projects AGL2010–16774 and CTQ2009–07430.

Supplementary material

11104_2011_840_MOESM1_ESM.doc (58 kb)
Suppl Fig. 1 Electropherogram of Amaranthus hybridus at 72 hours after treatment, and a time-amplified zone of the interval where the analytes appear. (DOC 57.5 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Hugo Cruz-Hipolito
    • 1
    • 5
    Email author
  • Antonia Rojano-Delgado
    • 1
  • José A. Domínguez-Valenzuela
    • 2
  • Antonio Heredia
    • 3
  • María Dolores Luque de Castro
    • 4
  • Rafael De Prado
    • 1
  1. 1.Department of Agricultural Chemistry and EdaphologyUniversity of CórdobaCórdobaSpain
  2. 2.Department of Agricultural ParasitologyChapingo Autonomous UniversityChapingoMexico
  3. 3.Department of Molecular Biology and BiochemistryUniversity of MálagaMálagaSpain
  4. 4.Department of Analytical Chemistry, Campus of RabanalesUniversity of CórdobaCórdobaSpain
  5. 5.Campus of RabanalesCórdobaSpain

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