Plant and Soil

, Volume 344, Issue 1–2, pp 389–400 | Cite as

Dynamic niche sharing in dry acidic grasslands -a 15N-labeling experiment

  • Vanessa Maliya Stahl
  • Wolfram Beyschlag
  • Christiane WernerEmail author
Regular Article


In this study we investigated the temporal variability of N-source utilization of pioneer plant species in different early successional stages of dry acidic grasslands. Current theory states that plant species occupy distinct ecological niches and that there are species-specific, temporal N-uptake patterns. We hypothesized that small-scale dynamics in the natural habitat may affect niche differentiation among plant species. We investigated N-uptake patterns of two co-occurring plant species from different functional groups (Corynephorus canescens, Rumex acetosella) under natural conditions using 15N-labeled nitrate and ammonium in three different early successional stages during early and late summer. We found (1) marked seasonal dynamics with respect to N-uptake and N-source partitioning, and (2) different uptake rates across successional stages but a similar N-form utilization of both species. Nitrate was the main N-source in the early and later successional stages, but a shift towards enhanced ammonium uptake occurred at the cryptogam stage in June. Both species increased N-uptake in the later successional stage in June, which was associated with increasing plant biomass in C. canescens, whereas R. acetosella showed no significant differences in plant biomass and root/shoot-ratio between successional stages. Ammonium uptake decreased in both species across all stages with increasing drought. Nevertheless, the peak time of N-uptake differed between the successional stages: in the early successional site, with the lowest soil N, plants were able to extend N-uptake into the drier season when uptake rates in the other successional stages had already declined markedly. Hence, we found a pronounced adjustment in the realized niches of co-occurring plant species with respect to N-uptake. Our results indicate that ecological niches can be highly dynamic and that niche sharing between plant species may occur instead of niche partitioning.


Ecological niches Nitrate Ammonium Succession Stable isotopes 



We would like to thank N. Krämer, M. Seither, T. Assaf, S. Unger, K. Ortmeier and others for help in the field; B. Teichner for laboratory assistance and K. G. Rascher for help with statistical questions and proofreading. Further we are grateful to A. Leßmann (LRA Fürth, Untere Naturschutzbehörde) for the possibility to do fieldwork at the nature protection area “Hainberg”. Helpful comments of A. Kahmen, M. Bartelheimer, B. Hartard and S. Unger and two anonymous referees are gratefully acknowledged.

All experiments comply with the current laws of the country in which they were performed.


  1. Ashton I, Miller A, Bowman W, Suding K (2008) Nitrogen preferences and plant-soil feedbacks as influenced by neighbors in the alpine tundra. Oecologia 156:625–636PubMedCrossRefGoogle Scholar
  2. Ashton I, Miller A, Bowman W, Suding K (2010) Niche complementarity due to plasticity in resource use: plant partitioning of chemical N forms. Ecology 91:3252–3260PubMedCrossRefGoogle Scholar
  3. Bartelheimer M, Steinlein T, Beyschlag W (2006) Aggregative root placement: a feature during interspecific competition in inland sand-dune habitats. Plant Soil 280:101–114CrossRefGoogle Scholar
  4. Bartelheimer M, Steinlein T, Beyschlag W (2008) 15N-nitrate-labelling demonstrates a size symmetric competitive effect on belowground resource uptake. Plant Ecol 199:243–253CrossRefGoogle Scholar
  5. Bertness MD, Callaway R (1994) Positive interactions in communities. Trends Ecol Evol 9:191–193PubMedCrossRefGoogle Scholar
  6. Beyschlag W, Hanisch S, Friedrich S, Jentsch A, Werner C (2009) 15N natural abundances during early and late succession in a middle-European dry acidic grassland. Plant Biol 11:713–724. doi: 10.1111/j.1438-8677.2008.00173.x PubMedCrossRefGoogle Scholar
  7. Binkley D, Vitousek P (1989) Soil nutrient availability. In: Pearcy RW, Ehleringer JR, Mooney HA, Rundel PW (eds) Plant physiological ecology—field methods and instrumentation. Chapman and Hall, London, pp 75–96Google Scholar
  8. Birch CPD, Hutchings MJ (1994) Exploitation of patchily distributed soil resources by the clonal herb Glechoma Hederacea. J Ecol 82:653–664CrossRefGoogle Scholar
  9. Bloom AJ, Sukrapanna SS, Warner RL (1992) Root respiration associated with ammonium and nitrate absorption and assimilation by barley. Plant Physiol 99:1294–1301PubMedCrossRefGoogle Scholar
  10. Bobbink R (1991) Effects of nutrient enrichment in Dutch chalk grassland. J Appl Ecol 28:28–41CrossRefGoogle Scholar
  11. Bobbink R, Hornung M, Roelofs JGM (1998) The effects of air-borne nitrogen pollutants on species diversity in natural and semi-natural European vegetation. J Ecol 86:717–738CrossRefGoogle Scholar
  12. Britto DT, Kronzucker HJ (2002) NH4+ toxicity in higher plants: a critical review. J Plant Physiol 159:567–584CrossRefGoogle Scholar
  13. Cain ML, Subler S, Evans JP, Fortin MJ (1999) Sampling spatial and temporal variation in soil nitrogen availability. Oecologia 118:397–404CrossRefGoogle Scholar
  14. Callaway RM, Nadkarni NM, Mahall BE (1991) Facilitation and interference of Quercus-Douglasii on understory productivity in Central California. Ecology 72:1484–1499CrossRefGoogle Scholar
  15. Callaway RM, Newingham B, Zabinski CA, Mahall BE (2001) Compensatory growth and competitive ability of an invasive weed are enhanced by soil fungi and native neighbours. Ecol Lett 4:429–433CrossRefGoogle Scholar
  16. Callaway RM, Brooker RW, Choler P, Kikvidze Z, Lortie CJ, Michalet R, Paolini L, Pugnaire FI, Newingham B, Aschehoug ET, Armas C, Kikodze D, Cook BJ (2002) Positive interactions among alpine plants increase with stress. Nature 417:844–848PubMedCrossRefGoogle Scholar
  17. Chapin FS, Walker LR, Fastie CL, Sharman LC (1994) Mechanisms of primary succession following deglaciation at Glacier Bay, Alaska. Ecol Monogr 64:149–175CrossRefGoogle Scholar
  18. Choler P, Michalet R, Callaway RM (2001) Facilitation and competition on gradients in alpine plant communities. Ecology 82:3295–3308CrossRefGoogle Scholar
  19. Colmer TD, Bloom AJ (1998) A comparison of NH4+ and NO3- net fluxes along roots of rice and maize. Plant Cell Environ 21:240–246CrossRefGoogle Scholar
  20. Cook WM, Yao J, Foster BL, Holt RD, Patrick LB (2005) Secondary succession in an experimentally fragmented landscape: community patterns across space and time. Ecology 86:1267–1279CrossRefGoogle Scholar
  21. de Kroon H, Hutchings MJ (1995) Morphological plasticity in clonal plants: the foraging concept reconsidered. J Ecol 83:143–152CrossRefGoogle Scholar
  22. Dukes JS (2001) Biodiversity and invasibility in grassland microcosms. Oecologia 126:563–568CrossRefGoogle Scholar
  23. Fargione J, Tilman D (2005) Niche differences in phenology and rooting depth promote coexistence with a dominant C-4 bunchgrass. Oecologia 143:598–606PubMedCrossRefGoogle Scholar
  24. Frey W, Hensen I (1995) Spergula morisonii—Corynphoretum canescentis (Frühlingspark-Silbergrasflur)—Lebensstrategien von Binnendünen- und Lockersandbesiedlern. Feddes Repert 106:533–553CrossRefGoogle Scholar
  25. Hutchinson GE (1957) Population studies—animal ecology and demography—concluding remarks. Cold Spring Harb Symp Quant Biol 22:415–427Google Scholar
  26. Jentsch A (2001) The significance of disturbance for vegetation dynamics. A case study in dry acidic grasslands. Dissertation, University of Bielefeld, GermanyGoogle Scholar
  27. Jentsch A, Friedrich S, Beyschlag W, Nezadal W (2002) Significance of ant and rabbit disturbances for seedling establishment in dry acidic grasslands dominated by Corynephorus canescens. Phytocoenologia 32:553–580CrossRefGoogle Scholar
  28. Jumpponen A, Hogberg P, Huss-Danell K, Mulder CPH (2002) Interspecific and spatial differences in nitrogen uptake in monocultures and two-species mixtures in north European grasslands. Funct Ecol 16:454–461CrossRefGoogle Scholar
  29. Kahmen A, Renker C, Unsicker SB, Buchmann N (2006) Niche complementarity for nitrogen: an explanation for the biodiversity and ecosystem functioning relationship? Ecology 87:1244–1255PubMedCrossRefGoogle Scholar
  30. Louahlia S, Macduff JH, Ourry A, Humphreys M, Boucaud J (1999) Nitrogen reserve status affects the dynamics of nitrogen remobilization and mineral nitrogen uptake during recovery of contrasting cultivars of Lolium perenne from defoliation. New Phytol 142:451–462CrossRefGoogle Scholar
  31. Maestre FT, Valladares F, Reynolds JF (2005) Is the change of plant-plant interactions with abiotic stress predictable? A meta-analysis of field results in arid environments. J Ecol 93:748–757CrossRefGoogle Scholar
  32. Marshall JK (1967) Biological flora of the British Isles—Corynephorus canescens (L.) Beauv. J Ecol 55:207–220CrossRefGoogle Scholar
  33. Marshall JK (1968) Factors limiting survival of Corynephorus Canescens (L) Beauv in Great Britain at northern edge of its distribution. Oikos 19:206–216CrossRefGoogle Scholar
  34. McKane RB, Grigal DF, Ruselle MP (1990) Spatiotemporal differences in 15N uptake and the organization of an old-field plant community. Ecology 71:1126–1132CrossRefGoogle Scholar
  35. McKane RB, Johnson LC, Shaver GR, Nadelhoffer KJ, Rastetter EB, Fry B, Giblin AE, Kielland K, Kwiatkowski BL, Laundre JA, Murray G (2002) Resource-based niches provide a basis for plant species diversity and dominance in arctic tundra. Nature 415:68–71PubMedCrossRefGoogle Scholar
  36. Miller AE, Bowman WD (2003) Alpine plants show species-level differences in the uptake of organic and inorganic nitrogen. Plant Soil 250:283–292CrossRefGoogle Scholar
  37. Miller AJ, Cramer MD (2004) Root nitrogen acquisition and assimilation. Plant Soil 274:1–36CrossRefGoogle Scholar
  38. Miller AE, Bowman WD, Suding KN (2007) Plant uptake of inorganic and organic nitrogen: neighbor identity matters. Ecology 88:1832–1840PubMedCrossRefGoogle Scholar
  39. Näsholm T (1998) Qualitative and quantitative changes in plant nitrogen acquisition induced by anthropogenic nitrogen deposition. New Phytol 139:87–90CrossRefGoogle Scholar
  40. Nordin A, Hogberg P, Nasholm T (2001) Soil nitrogen form and plant nitrogen uptake along a boreal forest productivity gradient. Oecologia 129:125–132CrossRefGoogle Scholar
  41. Oelmann Y, Wilcke W, Temperton VM, Buchmann N, Roscher C, Schumacher J, Schulze ED, Weisser WW (2007) Soil and plant nitrogen pools as related to plant diversity in an experimental grassland. Soil Sci Soc Am J 71:720–729CrossRefGoogle Scholar
  42. Peperkorn R, Werner C, Beyschlag W (2005) Phenotypic plasticity of an invasive Acacia versus two native Mediterranean species. Funct Plant Biol 32:933–944. doi: 10.1071/FP04197 CrossRefGoogle Scholar
  43. Radjaniemi TK (2007) Root foraging traits and competitive ability in heterogeneous soils. Oecologia 153:145–152CrossRefGoogle Scholar
  44. Schimel JP, Bennett J (2004) Nitrogen mineralization: challenges of a changing paradigm. Ecology 85:591–602CrossRefGoogle Scholar
  45. Smith SE, John BJ, Smith FA, Nicholas DJD (1985) Activity of glutamine synthetase and glutamate dehydrogenase in Trifolium subterraneum L. and Allium cepa L: effects of mycorrhizal infection and phosphyte nutrition. New Phytol 99:211–227CrossRefGoogle Scholar
  46. Spehn EM et al (2002) The role of legumes as a component of biodiversity in a cross-European study of grassland biomass nitrogen. Oikos 98:205–218CrossRefGoogle Scholar
  47. Süß K, Storm C, Zehm A, Schwabe A (2004) Succession in inland sand ecosystems: Which factors determine the occurrence of the tall grass species Calamagrostis epigejos (L.) Roth and Stipa capillata L.? Plant Biol 6:465–476PubMedCrossRefGoogle Scholar
  48. Tilman D (1988) Plant strategies and the dynamics and structure of plant communities. Princeton University Press, PrincetonGoogle Scholar
  49. Tilman D, Wedin D (1991) Plant traits and resource reduction for five grasses growing on a nitrogen gradient. Ecology 72:685–700CrossRefGoogle Scholar
  50. Tobar R, Azcon R, Barea JM (1994) Improved nitrogen uptake and transport from N-15-labeled nitrate by external hyphae of arbuscular mycorrhiza under water-stressed conditions. New Phytol 126:119–122CrossRefGoogle Scholar
  51. Vitousek PM, Howarth RW (1991) Nitrogen limitation on land and in the sea: how can it occur? Biogeochemistry 13:87–115CrossRefGoogle Scholar
  52. Vitousek PM, Aber JD, Howarth RW, Likens GE, Matson PA, Schindler DW, Schlesinger WH, Tilman DG (1997) Technical report: human alteration of the global nitrogen cycle: sources and consequences. Ecol Appl 7:737–750Google Scholar
  53. Von Felten S, Hector A, Buchmann N, Pascal PA, Schmid B, Scherer-Lorenzen M (2009) Belowground nitrogen partitioning in experimental grassland plant communities of varying species richness. Ecology 90:1389–1399CrossRefGoogle Scholar
  54. Weigelt A (2001) Plant competition on inland dunes: influence of water availability, nitrogen supply and the role of belowground processes. Dissertation, University of Bielefeld, GermanyGoogle Scholar
  55. Weigelt A, Steinlein T, Beyschlag W (2002) Does plant competition rather depend on biomass or species identity? Basic Appl Ecol 3:85–94CrossRefGoogle Scholar
  56. Weigelt A, Bol R, Bardgett RD (2005) Preferential uptake of soil nitrogen forms by grassland plant species. Oecologia 142:627–635PubMedCrossRefGoogle Scholar
  57. Weigelt A, Schumacher J, Walther T, Bartelheimer M, Steinlein T, Beyschlag W (2007) Identifying mechanisms of competition in multi-species communities. J Ecol 95:53–64CrossRefGoogle Scholar
  58. Werner C, Zumkier U, Beyschlag W, Máguas C (2010) High competitiveness of a resource demanding invasive acacia under low resource supply. Plant Ecol 206:83–96. doi: 10.1007/s11258-009-9625-0 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Vanessa Maliya Stahl
    • 1
  • Wolfram Beyschlag
    • 1
  • Christiane Werner
    • 1
    Email author
  1. 1.Department of Experimental and Systems EcologyUniversity of BielefeldBielefeldGermany

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