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Plant and Soil

, Volume 342, Issue 1–2, pp 445–457 | Cite as

Nitrogen partitioning between microbes and plants in the shortgrass steppe

  • Eliana E. Bontti
  • Ingrid C. Burke
  • William K. Lauenroth
Regular Article

Abstract

Nitrogen (N) and water additions in the shortgrass steppe change the dominance of plant functional types (PFT) that are characterized by different photosynthetic pathways and phenologies. We aimed to examine monthly patterns of plant N and microbial N storage during the growing season, and to assess whether N fertilization last applied 30 years ago alters the timing and magnitude of N storage. We measured plant biomass and N, and microbial biomass N monthly during the growing season. We found differences in temporal patterns of plant and microbial N storage in the control plots, with microbial storage higher than plant storage in July, and the opposite trend in September. Unlike the control plots, the plots fertilized 30 years ago exhibited overlapping peaks of N storage in plants and microbes in August. Seasonal trends indicated that rainfall was an important control over plant and microbial activity at the beginning of the growing season, and that temperature limited these activities at the end of the growing season. PFT affected the amount of microbial N, which was in general higher under C3 grasses than other PFTs, independent of fertilization. Historical resource additions increased plant biomass and N, but had little effect on microbial N. These results highlight the complexity of the microbial response. Changes in climate that influence precipitation timing will affect the temporal pattern for microbial biomass N, while management practices resulting in altered plant community composition will influence the magnitude of microbial biomass N.

Keywords

Grasslands Nitrogen partitioning Semi-arid ecosystems Shortgrass steppe Nitrogen availability Plant-microbe interactions 

References

  1. Aber JD, Nadelhoffer KJ, Steudler P, Melillo JM (1989) Nitrogen saturation in northern forest ecosystems. BioScience 39:378–386CrossRefGoogle Scholar
  2. Anderson JPE, Domsch KH (1975) Measurement of bacterial and fungal contributions to respiration of selected agricultural and forest soils. Can J Microbiol 21:314–322PubMedCrossRefGoogle Scholar
  3. Austin AT, Yahdjian L, Stark JM, Belnap J, Porporato A, Norton U, Ravetta DA, Schaeffer SM (2004) Water pulses and biogeochemical cycles in arid and semiarid ecosystems. Oecologia 141:221–235PubMedCrossRefGoogle Scholar
  4. Barrett JE, Burke IC (2000) Potential nitrogen immobilization in grassland soils across a soil organic matter gradient. Soil Biol Biochem 32:1707–1716CrossRefGoogle Scholar
  5. Barrett JE, Burke IC (2002) Nitrogen retention in semiarid ecosystems across a soil organic-matter gradient. Ecol Appl 12:878–890CrossRefGoogle Scholar
  6. Clark FE (1977) Internal cycling of N-15 in the shortgrass prairie. Ecology 58:1322–1333CrossRefGoogle Scholar
  7. Cookson WR, Osman M, Marschner P, Abaye DA, Clark I, Murphy DV, Stockdale EA, Watson CA (2007) Controls on soil nitrogen cycling and microbial community composition across land use and incubation temperature. Soil Biol Biochem 39:744–756CrossRefGoogle Scholar
  8. Corkidi L, Rowland DL, Johnson NC, Allen EB (2002) Nitrogen fertilization alters the functioning of arbuscular mycorrhizas at two semiarid grasslands. Plant Soil 240:299–310CrossRefGoogle Scholar
  9. Cui MY, Caldwell MM (1997) A large ephemeral release of nitrogen upon wetting of dry soil and corresponding root responses in the field. Plant Soil 191:291–299CrossRefGoogle Scholar
  10. de Vries FT, Hoffland E, van Eekeren N, Brussaard L, Bloem J (2006) Fungal/bacterial ratios in grasslands with contrasting nitrogen management. Soil Biol Biochem 38:2092–2103CrossRefGoogle Scholar
  11. Dell CJ, Williams MA, Rice CW (2005) Partitioning of nitrogen over five growing seasons in tallgrass prairie. Ecology 86:1280–1287CrossRefGoogle Scholar
  12. Dodd MB, Lauenroth WK, Burke IC, Chapman PL (2002) Associations between vegetation patterns and soil texture in the shortgrass steppe. Plant Ecol 158:127–137CrossRefGoogle Scholar
  13. Einsmann JC, Jones RH, Pu M, Mitchell RJ (1999) Nutrient foraging traits in 10 co-occurring plant species of contrasting life forms. J Ecol 87:609–619CrossRefGoogle Scholar
  14. Epstein HE, Burke IC, Mosier AR (1998) Plant effects on spatial and temporal patterns of nitrogen cycling in shortgrass steppe. Ecosystems 1:374–385CrossRefGoogle Scholar
  15. Fierer N, Allen AS, Schimel JP, Holden PA (2003) Control on microbial CO2 production: a comparison of surface and subsurface soil horizons. Global Change Biol 9:1322–1332CrossRefGoogle Scholar
  16. Gonzalez-Polo M, Austin AT (2009) Spatial heterogeneity provides organic matter refuges for soil microbial activity in the Patagonian steppe, Argentina. Soil Biol Biochem 41:1348–1351CrossRefGoogle Scholar
  17. Grayston SJ, Wang SQ, Campbell CD, Edwards AC (1998) Selective influence of plant species on microbial diversity in the rhizosphere. Soil Biol Biochem 30:369–378CrossRefGoogle Scholar
  18. Groffman PM, Eagan P, Sullivan WM, Lemunyon JL (1996) Grass species and soil type effects on microbial biomass and activity. Plant Soil 183:61–67CrossRefGoogle Scholar
  19. Groisman PY, Knight RW (2008) Prolonged dry episodes over the conterminous United States: new tendencies emerging over the last 40 years. J Climate 21:1850–1862CrossRefGoogle Scholar
  20. Hart SC, Stark JM, Davidson EA, Firestone MK (1994) Nitrogen mineralization, immobilization and nitrification. In: Weaver RW, Angle S, Bottomley P, Bezdicek D, Smith S, Tabatabai A, Wollum A (eds) Methods of soil analysis, Part 2, Microbiological and biochemical properties. Soil Science Society of America, Madison, pp 985–1018Google Scholar
  21. Hobbs RJ, Huenneke LF (1992) Disturbance, diversity, and invasion: implications for conservation. Conserv Biol 6:324–337CrossRefGoogle Scholar
  22. Högberg MN, Chen Y, Högberg P (2007) Gross nitrogen mineralisation and fungi-to-bacteria ratios are negatively correlated in boreal forests. Biol Fertil Soils 44:363–366CrossRefGoogle Scholar
  23. Hooper DU, Johnson L (1999) Nitrogen limitation in dryland ecosystems: Responses to geographical and temporal variation in precipitation. Biogeochemistry 46:247–293Google Scholar
  24. Jackson LE, Schimel JP, Firestone MK (1989) Short-term partitioning of ammonium and nitrate between plants and microbes in an annual grassland. Soil Biol Biochem 21:409–415CrossRefGoogle Scholar
  25. Jaeger CH, Monson RK, Fisk MC, Schmidt SK (1999) Seasonal partitioning of nitrogen by plants and soil microorganisms in an alpine ecosystem. Ecology 80:1883–1891CrossRefGoogle Scholar
  26. Johnson CK, Vigil MF, Doxtader KG, Beard WE (1996) Measuring bacterial and fungal substrate-induced respiration in dry soils. Soil Biol Biochem 28:427–432CrossRefGoogle Scholar
  27. Kaye JP, Hart SC (1997) Competition for nitrogen between plants and soil microorganisms. Trends Ecol Evol 12:139–143PubMedCrossRefGoogle Scholar
  28. Kennedy N, Brodie E, Connolly J, Clipson N (2004) Impact of lime, nitrogen and plant species on bacterial community structure in grassland microcosms. Environ Microbiol 6:1070–1080PubMedCrossRefGoogle Scholar
  29. Kennedy N, Connolly J, Clipson N (2005) Impact of lime, nitrogen and plant species on fungal community structure in grassland microcosms. Environ Microbiol 7:780–788PubMedCrossRefGoogle Scholar
  30. Lauenroth WK, Burke IC (2008) In: Lauenroth WK, Burke (eds) Ecology of the shortgrass steppe, Oxford University Press, NY, pp 3-14Google Scholar
  31. Lauenroth WK, Sala OE (1992) Long-term forage production of North-American shortgrass steppe. Ecol Appl 2:397–403CrossRefGoogle Scholar
  32. Lauenroth WK, Dodd JL, Sims PL (1978) Effects of water-induced and nitrogen-induced stresses on plant community structure in a semi-arid grassland. Oecologia 36:211–222CrossRefGoogle Scholar
  33. Lauenroth WK, Dodd JL, Dickinson CE (1980) Aboveground biomass dynamics of blue grama in a shortgrass steppe and evaluation of a method for separating live and dead. J Range Manage 33:210–212CrossRefGoogle Scholar
  34. Lauenroth WK, Sala OE, Milchunas DG, Lathrop RW (1987) Root dynamics of Bouteloua gracilis during short-term recovery from drought. Funct Ecol 1:117–124CrossRefGoogle Scholar
  35. Lin Q, Brookes PC (1999) Comparison of substrate induced respiration, selective inhibition and biovolume measurements of microbial biomass and its community structure in unamended, ryegrass-amended, fumigated and pesticide-treated soils. Soil Biol Biochem 31:1999–2014CrossRefGoogle Scholar
  36. Milchunas DG, Lauenroth WK (1995) Inertia in plant community structure - state changes after cessation of nutrient - enrichment stress. Ecol Appl 5:452–458CrossRefGoogle Scholar
  37. Milchunas DG, Lauenroth WK, Chapman PL, Kazempour MK (1989) Effects of grazing, topography, and precipitation on the structure of a semiarid grassland. Plant Ecol 80:11–23CrossRefGoogle Scholar
  38. Mulvaney RL (1996) Nitrogen-Inorganic forms. In: Sparks DL (ed) Methods of soil analysis, part 3, chemical methods. SSSA, Madison, pp 975–977Google Scholar
  39. Perry LG, Blumenthal DM, Monaco TA, Paschke MW, Redente EF (2010) Immobilizing nitrogen to control plant invasion. Oecologia 163:13–24PubMedCrossRefGoogle Scholar
  40. Porras-Alfaro A, Herrera J, Natvig DO, Sinsabaugh RL (2007) Effect of long-term nitrogen fertilization on mycorrhizal fungi associated with a dominant grass in a semiarid grassland. Plant Soil 296:65–75CrossRefGoogle Scholar
  41. Sala OE, Lauenroth WK, Parton WJ (1992) Long-term soil-water dynamics in the shortgrass steppe. Ecology 73:1175–1181CrossRefGoogle Scholar
  42. SAS Institute Inc (1989) SAS/STAT guide for personal computers: Version 6 edition. SAS Institute, IncGoogle Scholar
  43. Schwinning S, Sala OE (2004) Hierarchy of responses to resource pulses in arid and semi-arid ecosystems. Oecologia 141:211–220PubMedGoogle Scholar
  44. Sims PL, Singh JS (1978) Structure and function of 10 western North-American grasslands. 2. Intra-seasonal dynamics in primary producer compartments. J Ecol 66:547–572CrossRefGoogle Scholar
  45. Sylvia DM, Fuhrmann JJ, Hartel PG, Zuberer DA (2004) Principles and applications of soil microbiology. Prentice Hall, NJGoogle Scholar
  46. Vinton MA, Burke IC (1995) Interactions between individual plant - species and soil nutrient status in shortgrass steppe. Ecology 76:1116–1133CrossRefGoogle Scholar
  47. Vitousek PM, Howarth RW (1991) Nitrogen limitation on land and in the sea - how can it occur. Biogeochemistry 13:87–115CrossRefGoogle Scholar
  48. Vitousek PM, Aber J, Howarth RW, Likens GE, Matson PA, Schindler DW, Schlesinger WH, Tilman DG (1997) Human alteration of the global nitrogen cycle: causes and consequences. Ecol Appl 7:737–750Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Eliana E. Bontti
    • 1
    • 4
  • Ingrid C. Burke
    • 2
  • William K. Lauenroth
    • 3
  1. 1.Graduate Degree Program in EcologyColorado State UniversityFort CollinsUSA
  2. 2.Program in Environment and Natural Resources, Department of Botany, Department of Renewable Resources, and Program in EcologyUniversity of WyomingLaramieUSA
  3. 3.Department of Botany and Program in EcologyUniversity of WyomingLaramieUSA
  4. 4.Fort CollinsUSA

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