Plant and Soil

, Volume 342, Issue 1–2, pp 149–164 | Cite as

Effect of nitrogen on uptake, remobilization and partitioning of zinc and iron throughout the development of durum wheat

Regular Article

Abstract

Deficiencies of zinc (Zn) and iron (Fe) are global nutritional problems and caused most often by their limited dietary intake. Increasing Zn and Fe concentrations of staple food crops such as wheat is therefore an important global challenge. This study investigated the effects of varied nitrogen (N) and Zn supply on the total uptake, remobilization and partitioning of Zn, Fe and N in durum wheat throughout its ontogenesis. Plants were grown under greenhouse conditions with high or low supply of N and Zn, and harvested at 8 different developmental stages for analysis of Zn, Fe and N in leaves, stems, husks and grains. The results obtained showed that the Zn and Fe uptake per plant was enhanced up to 4-fold by high N supply while the increases in plant growth by high N supply were much less. When both the Zn and N supplies were high, approximately 50% of grain Zn and 80% of grain Fe were provided by post-anthesis shoot uptake, indicating that the contribution of remobilization to grain accumulation was higher for Zn than for Fe. At the high N and Zn application, about 60% of Zn, but only 40% of Fe initially stored in vegetative parts were retranslocated to grains, and nearly 80% of total shoot Zn and 60% of total shoot Fe were harvested with grains. All these values were significantly lower at the low N treatment. Results indicate that N nutrition is a critical factor in both the acquisition and grain allocation of Zn and Fe in wheat.

Keywords

Grain quality Iron Micronutrient deficiencies Nitrogen Wheat Zinc 

Notes

Acknowledgements

This study was financially supported by the HarvestPlus Biofortification Challenge Program (www.harvestplus.org) and the State Planning Organization of the Turkish Republic (http://www.dpt.gov.tr/ing/).

Supplementary material

11104_2010_679_MOESM1_ESM.doc (98 kb)
ESM 1 (DOC 98 kb)
11104_2010_679_MOESM2_ESM.doc (846 kb)
ESM 2 (DOC 846 kb)

References

  1. Black RE, Lindsay HA, Bhutta ZA, Caulfield LE, De Onnis M, Ezzati M, Mathers C, Rivera J (2008) Maternal and child undernutrition: global and regional exposures and health consequences. Lancet 371:243–260PubMedCrossRefGoogle Scholar
  2. Borg S, Brinch-Pedersen H, Tauris B, Holm PB (2009) Iron transport, deposition and bioavailability in the wheat and barley grain. Plant Soil 325:15–24CrossRefGoogle Scholar
  3. Bouis HE (2003) Micronutrient fortification of plants through plant breeding: can it improve nutrition in man at low cost? P Nutr Soc 62:403–411CrossRefGoogle Scholar
  4. Briat JF, Fobisloisy I, Grignon N, Lobreaux S, Pascal N, Savino N, Thoiron S, Vonwiren N, Vanwuytswinkel O (1995) Cellular and molecular aspects of iron metabolism in plants. Biol Cell 84:69–81CrossRefGoogle Scholar
  5. Cakmak I (2000) Role of zinc in protecting plant cells from reactive oxygen species. New Phytol 146:185–205CrossRefGoogle Scholar
  6. Cakmak I (2008) Enrichment of cereal grains with zinc: agronomic or genetic biofortification? Plant Soil 302:1–17CrossRefGoogle Scholar
  7. Cakmak I, Engels C (1999) Role of mineral nutrients in photosynthesis and yield formation. In: Rengel Z (ed) Crop nutrition. The Haworth Press, New York, pp 141–168Google Scholar
  8. Cakmak I, Yilmaz A, Ekiz H, Torun B, Erenoglu B, Braun HJ (1996) Zinc deficiency as a critical nutritional problem in wheat production in Central Anatolia. Plant Soil 180:165–172CrossRefGoogle Scholar
  9. Cakmak I, Kalayci M, Ekiz H, Braun HJ, Yilmaz A (1999) Zinc deficiency as a practical problem in plant and human nutrition in Turkey: a NATO-Science for Stability project. Field Crops Res 60:175–188CrossRefGoogle Scholar
  10. Cakmak I, Pfeiffer WH, McClafferty B (2010a) Biofortification of durum wheat with zinc and iron. Cereal Chem 87:10–20CrossRefGoogle Scholar
  11. Cakmak I, Kalayci M, Kaya Y, Torun AA, Aydin N, Wang Y, Arisoy Z, Erdem H, Gokmen O, Ozturk L, Horst WJ (2010b) Biofortification and localization of zinc in wheat grain. J Agric Food Chem 58:9092–9102Google Scholar
  12. Curie C, Cassin G, Couch D, Divol F, Higuchi K, Jean ML, Misson J, Schikora A, Czernic P, Mari S (2009) Metal movement within the plant: contribution of nicotianamine and yellow stripe 1-like transporters. Ann Bot—London 103:1–11CrossRefGoogle Scholar
  13. Distelfeld A, Cakmak I, Peleg Z, Ozturk L, Yazici AM, Budak H, Saranga Y, Fahima T (2007) Multiple QTL-effects of wheat Gpc-B1 locus on grain protein and micronutrient concentrations. Physiol Plant 29:635–643CrossRefGoogle Scholar
  14. Erdal I, Yilmaz A, Taban S, Eker S, Cakmak I (2002) Phytic acid and phosphorus concentrations in seeds of wheat cultivars grown with and without zinc fertilization. J Plant Nutr 25:113–127CrossRefGoogle Scholar
  15. Erenoglu B, Nikolic M, Romheld V, Cakmak I (2002) Uptake and transport of foliar applied zinc (65Zn) in bread and durum wheat cultivars differing in zinc efficiency. Plant Soil 241:251–257CrossRefGoogle Scholar
  16. Ficco DBM, Riefolo C, Nicastro G, Simone VD, Gesù AM, Beleggia R, Platani C, Cattivelli L, Vita PD (2009) Phytate and mineral elements concentration in a collection of Italian durum wheat cultivars. Field Crops Res 111:235–242CrossRefGoogle Scholar
  17. Fraga CG (2005) Relevance, essentiality and toxicity of trace elements in human health. Mol Aspects Med 26:235–244PubMedCrossRefGoogle Scholar
  18. Garnett TP, Graham RD (2005) Distribution and remobilization of iron and copper in wheat. Ann Bot—London 95:817–826CrossRefGoogle Scholar
  19. Grusak MA, Pearson JN, Marentes E (1999) The physiology of micronutrient homeostasis in field crops. Field Crops Res 60:41–56CrossRefGoogle Scholar
  20. Gupta UC (1991) Iron statues of crops in Prince-Edward-Island and effect of soil-pH on plant iron concentration. Can J Soil Sci 71:197–202Google Scholar
  21. Haslett BS, Reid RJ, Rengel Z (2001) Zinc mobility in wheat: uptake and distribution of zinc applied to leaves or roots. Ann Bot—London 87:379–386CrossRefGoogle Scholar
  22. Haydon MJ, Cobbett CS (2007) Transporters of ligands for essential metal ions in plants. New Phytol 174:499–506PubMedCrossRefGoogle Scholar
  23. Himelblau E, Amasino RM (2001) Nutrients mobilized from leaves of Arabidopsis thaliana during leaf senescence. J Plant Physiol 158:1317–1323CrossRefGoogle Scholar
  24. Hocking PJ (1994) Dry-matter production, mineral nutrient concentrations, and nutrient distribution and redistribution in irrigated spring wheat. J Plant Nutr 17:1289–1308CrossRefGoogle Scholar
  25. Hotz C, Brown KH (2004) Assessment of the risk of zinc deficiency in populations and options for its control. Food Nutr Bull 25:94–204Google Scholar
  26. Kennedy G, Nantel G, Shetty P (2003) The scourge of “hidden hunger”: global dimensions of micronutrient deficiencies. Food Nutr Agric (FAO FNA) 32:8–16Google Scholar
  27. Kichey T, Hirel B, Heumez E, Dubois F, Le Gouis J (2007) In winter wheat (Triticum aestivum L.), post-anthesis nitrogen uptake and remobilisation to the grain correlates with agronomic traits and nitrogen physiological markers. Field Crops Res 102:22–32CrossRefGoogle Scholar
  28. Kruger C, Berkowitz O, Stephan UW, Hell R (2002) A metal-binding member of the late embryogenesis abundant protein family transports iron in the phloem of Ricinus communis L. J Biol Chem 277:25062–25069PubMedCrossRefGoogle Scholar
  29. Kutman UB, Yildiz B, Ozturk L, Cakmak I (2010) Biofortification of durum wheat with zinc through soil and foliar applications of nitrogen. Cereal Chem 87:1–9CrossRefGoogle Scholar
  30. Lindsay WL, Norvell WA (1978) Development of a DTPA soil test for zinc, iron, manganese and copper. Soil Sci Soc Am J 42:421–428CrossRefGoogle Scholar
  31. Marschner H (1993) Zinc uptake from soils. In: Robson AD (ed) Zinc in soils and plants. Kluwer Academic Publishers, Dordrecht, pp 59–77Google Scholar
  32. Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic, LondonGoogle Scholar
  33. Marschner H, Romheld V (1994) Strategies of plants for acquisition of iron. Plant Soil 165:261–274CrossRefGoogle Scholar
  34. Miller RO, Jacobsen JS, Skogley EO (1994) Aerial accumulation and partitioning of nutrients by hard red spring wheat. Commun Soil Sci Plan 25:1891–1911CrossRefGoogle Scholar
  35. Morgounov A, Gomez-Becerra HF, Abugalieva A, Dzhunusova M, Yessimbekova M, Muminjanov H, Zelenskiy Y, Ozturk L, Cakmak I (2007) Iron and zinc grain density in common wheat grown in Central Asia. Euphytica 155:193–203CrossRefGoogle Scholar
  36. Mori S, Nishizawa N (1987) Methionine as a dominant precursor of phytosiderophores in Graminaceae plants. Plant Cell Physiol 28:1081–1092Google Scholar
  37. Niles BJ, Clegg MS, Hanna LA, Chou SS, Momma TY, Hong H, Keen CL (2008) Zinc deficiency-induced iron accumulation: a consequence of alterations, iron regulatory protein binding activity, iron transporters, and iron storage proteins. J Biol Chem 283:5168–5177PubMedCrossRefGoogle Scholar
  38. Ozturk L, Yazici MA, Yucel C, Torun A, Cekic C, Bagci A, Ozkan H, Braun H-J, Sayers Z, Cakmak I (2006) Concentration and localization of zinc during seed development and germination in wheat. Physiol Plantarum 128:144–152CrossRefGoogle Scholar
  39. Palmer CM, Guerinot ML (2009) Facing the challenges of Cu, Fe and Zn homeostasis in plants. Nat Chem Biol 5:333–340PubMedCrossRefGoogle Scholar
  40. Pearson JN, Rengel Z (1994) Distribution and remobilization of Zn and Mn during grain development in wheat. J Exp Bot 45:1829–1835CrossRefGoogle Scholar
  41. Pearson JN, Rengel Z, Jenner CF, Graham RD (1995) Transport of zinc and manganese to developing wheat grains. Physiol Plant 95:449–455CrossRefGoogle Scholar
  42. Peleg Z, Saranga Y, Yazici MA, Fahima T, Ozturk L, Cakmak I (2008) Grain zinc, iron and protein concentrations and zinc-efficiency in wild emmer wheat under contrasting irrigation regimes. Plant Soil 306:57–67CrossRefGoogle Scholar
  43. Persson DP, Hansen TH, Laursen KH, Schjoerring JK, Husted S (2009) Simultaneous iron, zinc, sulfur and phosphorus speciation analysis of barley grain tissues using SEC-ICP-MS and IP- ICP-MS. Metallomics 1:418–426PubMedCrossRefGoogle Scholar
  44. Pfeiffer WH, McClafferty B (2007) Biofortification: breeding micronutrient-dense crops. In: Kang MS, Priyadarshan PM (eds) Breeding major food staples. Blackwell Science, New York, pp 61–91CrossRefGoogle Scholar
  45. Sharma PN, Chatterjee C, Agarwala SC, Sharma CP (1990) Zinc deficiency and pollen fertility in maize (Zea mays). Plant Soil 124:221–225CrossRefGoogle Scholar
  46. Shi R, Zhang Y, Chen X, Sun Q, Zhang F, Romheld V, Zou C (2010) Influence of long-term nitrogen fertilization on micronutrient density in grain of winter wheat (Triticum aestivum L.). J Cereal Sci 51:165–170CrossRefGoogle Scholar
  47. Suzuki M, Tsukamato T, Inoue H, Watanabe S, Matsuhashi S, Takahashi M, Nakanishi H, Mori S, Nishizawa NK (2008) Deoxymugineic acid increases Zn translocation in Zn-deficienct rice plants. Plant Mol Biol 66:609–617PubMedCrossRefGoogle Scholar
  48. Trampczynska A, Küpper H, Meyer-Klaucke M, Schmidt H, Clemens S (2010) Nicotianamine forms complexes with Zn(II) in vivo. Metallomics 2:57–66PubMedCrossRefGoogle Scholar
  49. Tsukamoto T, Nakanishi H, Uchida H, Watanabe S, Matsuhashi S, Mori S, Nishizawa NK (2009) Fe-52 translocation in barley as monitored by a positron-emitting tracer imaging system (PETIS): Evidence for the direct translocation of Fe from roots to young leaves via phloem. Plant Cell Physiol 50:48–57PubMedCrossRefGoogle Scholar
  50. Uauy C, Brevis JC, Dubcovsky J (2006a) The high grain protein content gene Gpc-B1 accelerates senescence and has pleiotropic effects on protein content in wheat. J Exp Bot 57:2785–2794PubMedCrossRefGoogle Scholar
  51. Uauy C, Distelfeld A, Fahima T, Blechl A, Dubcovsky J (2006b) A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science 314:1298–1301PubMedCrossRefGoogle Scholar
  52. Warnock RE (1970) Micronutrient uptake and mobility within corn plants (Zea mays L.) in relation to phosphorus-induced zinc deficiency. Soil Sci Soc Am J 34:765–769CrossRefGoogle Scholar
  53. Waters BM, Grusak MA (2008) Whole-plant mineral partitioning throughout the life cycle in Arabidopsis thaliana ecotypes Columbia, Landsberg erecta, Cape Verde Islands, and the mutant line ysl1ysl3. New Phytol 177:389–405PubMedGoogle Scholar
  54. Waters BM, Chu H-H, DiDonato RJ, Roberts LA, Eisley RB, Lahner B, Salt DE, Walker EL (2006) Mutations in Arabidopsis yellow stripe-like1 and yellow stripe-like3 reveal their roles in metal ion homeostasis and loading of metal ions in seeds. Plant Physiol 141:1446–1458PubMedCrossRefGoogle Scholar
  55. Waters BM, Uauy C, Dubcovsky J, Grusak MA (2009) Wheat (Triticum aestivum) NAM proteins regulate the translocation of iron, zinc, and nitrogen compounds from vegetative tissues to grain. J Exp Bot 60:4263–4274PubMedCrossRefGoogle Scholar
  56. Welch RM, Graham RD (2004) Breeding for micronutrients in staple food crops from a human nutirion perspective. J Exp Bot 55:353–364PubMedCrossRefGoogle Scholar
  57. White PJ, Broadley MR (2005) Biofortifying crops with essential mineral elements. Trends Plant Sci 10:586–593PubMedCrossRefGoogle Scholar
  58. White PJ, Broadley MR (2009) Biofortification of crops with seven mineral elements often lacking in human diets—iron, zinc, copper, calcium, magnesium, selenium and iodine. New Phytol 182:49–84PubMedCrossRefGoogle Scholar
  59. Yang J, Zhang J (2006) Grain filling of cereals under soil drying. New Phytol 169:223–236PubMedCrossRefGoogle Scholar
  60. Yilmaz A, Ekiz H, Torun B, Gültekin I, Karanlik S, Bagci SA, Cakmak I (1997) Effect of different zinc application methods on grain yield and zinc concentration in wheat grown on zinc-deficient calcareous soils in Central Anatolia. J Plant Nutr 20:461–471CrossRefGoogle Scholar
  61. Zhao FJ, Su YH, Dunham SJ, Rakszegi M, Bedo Z, McGrath SP, Shewry PR (2009) Variation in mineral micronutrient concentrations in grain of wheat lines of diverse origin. J Cereal Sci 49:290–295CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Umit Baris Kutman
    • 1
  • Bahar Yildiz
    • 1
  • Ismail Cakmak
    • 1
  1. 1.Faculty of Engineering and Natural SciencesSabanci UniversityIstanbulTurkey

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