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Plant and Soil

, Volume 342, Issue 1–2, pp 117–128 | Cite as

Root plasticity as the key root trait for adaptation to various intensities of drought stress in rice

  • Mana Kano
  • Yoshiaki Inukai
  • Hidemi Kitano
  • Akira Yamauchi
Regular Article

Abstract

Roots play an important role in rice adaptation to drought conditions. This study aimed to identify the key root traits that contribute to plant adaptation to drought stress. We used chromosome segment substitution lines (CSSLs) derived from Nipponbare and Kasalath crosses, which were grown in the field and hydroponics. In field experiments, the plants were grown under soil moisture gradients with line source sprinkler system up to around heading. Among the 54 CSSLs, only CSSL50 consistently showed significantly higher shoot dry matter production than its parent Nipponbare as the drought intensified for 3 years while most of the CSSLs reduced dry matter production to similar extents with Nipponbare under the same conditions. CSSL50 showed significantly greater total root length through promoted lateral root branching and elongation than Nipponbare, especially under mild stress conditions (15−30% w/w of soil moisture contents), which is considered as phenotypic plasticity. Such plastic root development was the key trait that effectively contributed to plant dry matter production through increased total root length and thus water uptake. However, there was no relationship between root plasticity and plant growth under the stress conditions induced by polyethylene glycol in hydroponics.

Keywords

Chromosome Segment Substitution Lines (CSSLs) QTL Rainfed lowland Root plasticity Water deficit 

Abbreviations

CSSLs

Chromosome segment substitution lines

DAT

Days after transplanting

NILs

Near isogenic lines

PEG

Polyethylene glycol

SMC

Soil moisture content

Notes

Acknowledgments

We thank Professor Shu Fukai of the University of Queensland for a critical review and useful comments on our manuscript. This research was supported by Grant-in-Aid for Scientific Research (No. 22380013) and Grant-in-Aid for JSPS Fellows (No. 21007569) from the Japan Society for the Promotion of Science, and a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics for Agricultural Innovation, QTL-4004).

References

  1. Azhiri-Sigari T, Yamauchi A, Kamoshita A, Wade LJ (2000) Genotypic variation in response of rainfed lowland rice to drought and rewatering. II. Root growth. Plant Prod Sci 3:180–188CrossRefGoogle Scholar
  2. Bañoc DM, Yamauchi A, Kamoshita A, Wade LJ, Pardales JR Jr (2000a) Dry matter production and root system development of rice cultivars under fluctuating soil moisture. Plant Prod Sci 3:197–207CrossRefGoogle Scholar
  3. Bañoc DM, Yamauchi A, Kamoshita A, Wade LJ, Pardales JR Jr (2000b) Genotypic variations in response of lateral root development to fluctuating soil moisture in rice. Plant Prod Sci 3:335–343CrossRefGoogle Scholar
  4. Bernier J, Serraj R, Kumar A, Venuprasad R, Impa S, Gowda V, Owane R, Spaner D, Atlin G (2009) The large-effect drought-resistance QTL qtl12.1 increases water uptake in upland rice. Field Crop Res 110:139–146CrossRefGoogle Scholar
  5. Carpita C, Sabularse D, Montezinos D, Delmer DP (1979) Determination of pore size of cell walls of living plant sells. Science 205:1144–1149PubMedCrossRefGoogle Scholar
  6. Cui KH, Huang JL, Xing YZ, Yu SB, Xu CG, Peng SB (2008) Mapping QTLs for seedling characteristics under different water supply conditions in rice (Oryza sativa). Physiol Plant 132:53–68PubMedGoogle Scholar
  7. Fukai S, Cooper M (1995) Development of drought-resistant cultivars using physio-morphological traits in rice. Field Crops Res 40:67–86CrossRefGoogle Scholar
  8. Fukai S, Basnayake J, Ouk M (2008) Drought resistance characters and variety development for rainfed lowland rice in Southeast Asia. In: Serraj R, Bennett J, Hardy B (eds) Drought frontiers in rice: crop improvement for increased rainfed production. World Scientific, Singapore, pp 75–89Google Scholar
  9. Guo Y, Ma YT, Zhan ZG, Li BG, Dingkuhn M, Luquet D, De Reffye P (2006) Parameter optimization and field validation of the functional-structural model GREENLAB for maize. Ann Bot 97:217–230PubMedCrossRefGoogle Scholar
  10. Haefele SM, Jabbar SMA, Siopongco JDLC, Tirol-Padre A, Amarante ST, Cruz PCS, Cosico WC (2008) Nitrogen use efficiency in selected rice (Oryza sativa L.) genotypes under different water regimes and nitrogen levels. Field Crops Res 107:137–146CrossRefGoogle Scholar
  11. Hodge A (2004) The plastic plant: root responses to heterogeneous supplies of nutrients. New Phytol 162:9–24CrossRefGoogle Scholar
  12. Huang Y, Zhao X, Zhang H, Huang G, Luo Y, Japhet W (2009) A comparison of phenotypic plasticity between two species occupying different positions in a successional sequence. Ecol Res 24:1335–134CrossRefGoogle Scholar
  13. Ingram KT, Bueno FD, Namuco OS, Yambao EB, Betrouty CA (1994) Rice root traits for drought resistance and their genetic variation. In: Kirk GJD (ed) Rice root: nutrient and water use. International Rice Research Institute, Manila, pp 66–77Google Scholar
  14. Ito K, Tanakamaru K, Morita S, Abe J, Inanaga S (2006) Lateral root development, including responses to soil drying, of maize (Zea mays) and wheat (Triticum aestivum) seminal roots. Physiol Plant 127:260–267CrossRefGoogle Scholar
  15. Kamoshita A, Wade LJ, Yamauchi A (2000) Genotypic variation in response of rainfed lowland rice to drought and rewatering. III. Water extraction during drought period. Plant Prod Sci 3:189–196CrossRefGoogle Scholar
  16. Kamoshita A, Zhang J, Siopongco JDLC, Sarkarung S, Nguyen HT, Wade LJ (2002a) Effects of phenotyping environment on identification of QTL for rice root morphology under anaerobic conditions. Crop Sci 42:255–265PubMedCrossRefGoogle Scholar
  17. Kamoshita A, Wade LJ, Ali ML, Pathan MS, Zhang J, Sarkarung S, Nguyen HT (2002b) Mapping QTLs for root morphology of a rice population adapted to rainfed flooding conditions. Theor Appl Genet 104:880–893PubMedCrossRefGoogle Scholar
  18. Kamoshita A, Babu RC, Boopathi NM, Fukai S (2008) Phenotypic and genotypic analysis of drought-resistance traits for development of rice cultivars adapted to rainfed environments. Field Crop Res 109:1–23CrossRefGoogle Scholar
  19. Kang SY, Morita S, Yamazaki K (1994) Root growth and distribution in some japonica-indica hybrid and japonica type rice cultivars under field conditions. Jpn J Crop Sci 63:118–124Google Scholar
  20. Kano M, Inukai Y, Yamauchi A (2009) Evaluation of functional roles of root plasticity in shoot dry matter production under drought stress by using CSSLs in rice. Jpn J Crop Sci (Extra 1) 78:276–277, in Japanese with English summaryGoogle Scholar
  21. Kato Y, Abe J, Kamoshita A, Yamagishi J (2006) Genotypic variation in root growth angle in rice (Oryza sativa L.) and its association with deep root development in upland fields with different water regimes. Plant Soil 287:117–129CrossRefGoogle Scholar
  22. Kato Y, Kamoshita A, Yamagishi J (2007) Evaluating the resistance of six rice cultivars to drought: root restriction and the use of raised beds. Plant Soil 300:149–161CrossRefGoogle Scholar
  23. Kimura K, Yamasaki S (2001) Root length and diameter measurement using NIH Image: application of the line-principal for diameter estimation. Plant Soil 234:37–46CrossRefGoogle Scholar
  24. Kimura K, Kikuchi S, Yamasaki S (1999) Accurate root length measurement by image analysis. Plant Soil 216:117–127CrossRefGoogle Scholar
  25. Lanceras JC, Pantuwan G, Jongdee B, Toojinda T (2004) Quantitative trait loci associated with drought tolerance at reproductive stage in rice. Plant Physiol 135:384–399PubMedCrossRefGoogle Scholar
  26. Maclean JL, Dawe D, Hardy B, Hettel GP (eds) (2002) Rice Almanac IRRI, WARDA, CIAT, FAO, Los Baños (Philippines), Bouaké (Côte d’Ivoire), Cali (Colombia) and Rome (Italy) pp 1–253Google Scholar
  27. Nguyen TTT, Klueva N, Chamareck V, Aarti A, Magpantay G, Millena ACM, Pathan MS, Nguyen HT (2004) Saturation mapping of QTL regions and identification of putative candidate genes for drought tolerance in rice. Mol Genet Genomics 272:35–46PubMedCrossRefGoogle Scholar
  28. O’Toole JC, Bland WL (1987) Genotypic variation in crop plant root system. Adv Agron 41:91–145CrossRefGoogle Scholar
  29. Rachmilevitch S, Huang B, Lambers H (2006) Assimilation and allocation of carbon and nitrogen of thermal and nonthermal Agrostis species in response to high soil temperature. New Phytol 170:479–490PubMedCrossRefGoogle Scholar
  30. Robinson D (2001) Root proliferation, nitrate inflow and their carbon costs during nitrogen capture by competing plants in patchy soil. Plant Soil 232:41–50CrossRefGoogle Scholar
  31. Serraj R, Kumar A, McNally KL, Slamet-Loedin I, Bruskiewich R, Mauleon R, Cairns J, Hijmans RJ (2009) Improvement of drought resistance in rice. Adv Agron 103:41–99CrossRefGoogle Scholar
  32. Shimizu H, Maruoka M, Ichikawa N, Baruah AR, Uwatoko N, Sano Y, Onishi K (2010) Genetic control of phenotypic plasticity in Asian cultivated and wild rice in response to nutrient and density changes. Genome 53:211–223PubMedCrossRefGoogle Scholar
  33. Siopongco JDLC, Yamauchi A, Salekdeh H, Bennett J, Wade LJ (2005) Root growth and water extraction responses of doubled-haploid rice lines to drought and rewatering during the vegetative stage. Plant Prod Sci 8:497–508CrossRefGoogle Scholar
  34. Siopongco JDLC, Yamauchi A, Salekdeh H, Bennett J, Wade LJ (2006) Growth andwater use response of doubled-haploid rice lines to drought and rewatering during the vegetative stage. Plant Prod Sci 9:141–151CrossRefGoogle Scholar
  35. Subere JOQ, Bolatete D, Bergantin R, Pardales A, Belmonte JJ, Mariscal A, Sebidos R, Yamauchi A (2009) Genotypic variation in responses of cassava (Manihot esculenta Crantz) to drought and rewatering. I. Root system development. Plant Prod Sci 12:462–474CrossRefGoogle Scholar
  36. Suralta RR, Yamauchi A (2008) Root growth, aerenchyma development, and oxygen transport in rice genotypes subjected to drought andwaterlogging. Environ Exp Bot 64:75–82CrossRefGoogle Scholar
  37. Suralta RR, Inukai Y, Yamauchi A (2008a) Genotypic variations in responses of lateral root development to transient moisture stresses in rice cultivars. Plant Prod Sci 11:324–335CrossRefGoogle Scholar
  38. Suralta RR, Inukai Y, Yamauchi A (2008b) Utilizing chromosome segment substitution lines (CSSLs) for evaluation of root responses under transient moisture stresses in rice. Plant Prod Sci 11:457–465CrossRefGoogle Scholar
  39. Suralta RR, Inukai Y, Yamauchi A (2010) Dry matter production in relation to root plastic development, oxygen transport and water uptake of rice under transient soil moisture stresses. Plant Soil 332:87–104CrossRefGoogle Scholar
  40. Valladares F, Dobarro I, Sanchez-Gomez D, Pearcy RW (2005) Photoinhibition and drought in Mediterranean woody saplings: scaling effects and interactions in sun and shade phenotypes. J Exp Bot 56:483–494PubMedCrossRefGoogle Scholar
  41. Verslues PE, Ober ES, Sharp RE (1998) Root growth and oxygen relations at low water potentials. Impact of oxygen availability in polyethylene glycol solutions. Plant Physiol 116:1403–1412PubMedCrossRefGoogle Scholar
  42. Wade LJ, Fukai S, Samson BK, Ali A, Mazid MA (1999) Rainfed lowland rice: physical environment and cultivar requirements. Field Crop Res 64:3–12CrossRefGoogle Scholar
  43. Wang H, Yamauchi A (2006) Growth and function of roots under abiotic stress soils. In: Huang B (ed) Plant-Environment Interactions, 3rd edn. CRC, Taylor and Francis Group, LLC, New York, pp 271–320CrossRefGoogle Scholar
  44. Wang H, Siopongco JDLC, Wade LJ, Yamauchi A (2009) Fractal analysis on root systems of rice plants in response to drought stress. Environ Exp Bot 65:338–344CrossRefGoogle Scholar
  45. Yamauchi A (2004) Root system. In: Yamazaki K et al (eds) Encyclopedia of agricultural sciences. Yokendo, Tokyo, pp 668–675 (In Japanese)Google Scholar
  46. Yamauchi A, Kono Y, Tatsumi J (1987) Quantitative analysis on root system structures of upland rice and maize. Jpn J Crop Sci 56:608–617Google Scholar
  47. Yamauchi A, Pardales JR Jr, Kono Y (1996) Root system structure and its relation to stress tolerance. In: Ito O, Katayama K, Johansen C, Kumar Rao JVDK, Adu-Gyamfi JJ, Rego TJ (eds) Dynamics of roots and nitrogen in cropping systems of the semi-arid tropics. JIRCAS, Tsukuba, pp 211–234Google Scholar
  48. Yue B, Xue W, Xiong L, Yu Z, Luo L, Cui K, Jin D, Xing Y, Zhang Q (2006) Genetic basis of drought resistance at reproductive stage in rice: separation of drought resistance from drought avoidance. Genetics 172:1213–1228PubMedCrossRefGoogle Scholar
  49. Zhang J, Zheng HG, Aarti A, Pantuwan G, Nguyen TT, Tripathy JN, Sarial AK, Robin S, Babu RC, Nguyen BD, Sarkarung S, Blum A, Nguyen HT (2001) Locating genomic regions associated with components of drought resistance in rice: comparative mapping within and across species. Theor Appl Genet 103:19–29CrossRefGoogle Scholar
  50. Zhang H, Xue YG, Wang ZQ, Yang J, Zhang JH (2009) Morphological and physiological traits of roots and their relationships with shoot growth in “super” rice. Field Crops Res 113:31–40CrossRefGoogle Scholar
  51. Zheng BS, Yang L, Zhang WP, Mao CZ, Wu YR, Yi KK, Liu FY, Wu P (2003) Mapping QTLs and candidate genes for rice root traits under different water-supply conditions and comparative analysis across three populations. Theor Appl Genet 107:1505–1515PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Mana Kano
    • 1
  • Yoshiaki Inukai
    • 1
  • Hidemi Kitano
    • 2
  • Akira Yamauchi
    • 1
  1. 1.Graduate School of Bioagricultural SciencesNagoya UniversityNagoyaJapan
  2. 2.Bioscience and Biotechnology CenterNagoya UniversityNagoyaJapan

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