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Plant and Soil

, Volume 336, Issue 1–2, pp 251–265 | Cite as

Glyphosate affects photosynthesis in first and second generation of glyphosate-resistant soybeans

  • Luiz Henrique Saes Zobiole
  • Robert John Kremer
  • Rubem Silvério de OliveiraJr
  • Jamil Constantin
Regular Article

Abstract

The crop area planted to conventional soybeans has decreased annually while that planted to glyphosate-resistant (RR) soybean has drastically increased mainly due to the wide adoption of glyphosate in current weed management systems. With the extensive use of glyphosate, many farmers have noted visual plant injury in RR soybean varieties after glyphosate application. A new generation designated as “second generation—RR2” has been recently developed and these RR2 cultivars already are commercially available for farmers and promoted as higher yielding relative to the previous RR cultivars. However, little information is currently available about the performance of RR2 soybean beyond commercial and farmer testimonial data. Thus, an evaluation of different glyphosate rates applied in different growth stages of the first and second generation of RR soybeans, revealed a significant decrease in photosynthesis. In general, increased glyphosate rate and late applications (V6) pronounced decrease photosynthetic parameters and consequently decreased in leaf area and shoot biomass production. In contrast, low rate and early applications were less damage for the RR soybean plants, suggesting that with early applications (V2), plants probably have more time to recover from glyphosate or its metabolites effects regarding late applications.

Keywords

Glyphosate resistant soybean (Glycine max L.) Glyphosate Photosynthesis Biomass 

Abbreviations

DAS

Days after sowing

A

Photosynthetic rate

E

Transpiration rate

gs

Stomatal conductance

Ci

Sub-stomatal CO2

ETR

Photosynthetic electron transport rates

Fo′

Minimal fluorescence of a light adapted leaf

Fm′

Maximal fluorescence of a light adapted leaf

Fs

Steady state fluorescence of a light adapted leaf

Fv′/Fm′

Intrinsic efficiency of photosystem 2

PS2

Photosystem 2

PhiPS2

Quantum efficiencies of photosynthetic electron transport through photosystem 2

PhiCO2

Quantum yield based on CO2 assimilation

qN

Non-photochemical quenching of chlorophyll fluorescence

qP

Proportion of open reaction centers

RR1

Glyphosate-resistant soybean—first generation

RR2

Glyphosate-resistant soybean—second generation

Non-RR

Conventional soybean near-isogenic parental line

Notes

Acknowledgements

We thank the National Council for Scientific and Technology Development (CNPq-Brasilia, DF, Brazil) for the scholarship and financial support for this research. The authors also thank Dr. Bruce Hibbard, USDA, Agricultural Research Service for use of greenhouse facilities and Carey Page, University of Missouri for assistance with herbicide applications. Trade names are used for clarity and do not represent endorsement by USDA-ARS, the State University of Maringá, or the University of Missouri.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Luiz Henrique Saes Zobiole
    • 1
  • Robert John Kremer
    • 2
  • Rubem Silvério de OliveiraJr
    • 1
  • Jamil Constantin
    • 1
  1. 1.Center for Advanced Studies in Weed Research (NAPD)State University of Maringá (UEM)MaringáBrazil
  2. 2.United States Department of Agriculture, Agricultural Research Service, Cropping Systems & Water Quality Research UnitUniversity of MissouriColumbiaUSA

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