Siderophore and chitinase producing isolates from the rhizosphere of Nicotiana glauca Graham enhance growth and induce systemic resistance in Solanum lycopersicum L.
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A screening for Plant Growth Promoting Rhizobacteria (PGPR) was carried out in the rhizosphere of wild populations of Nicotiana glauca Graham in south-eastern Spain. Nine hundred and sixty strains were isolated and grouped in four parataxonomic groups: Gram positive endospore forming bacilli, Gram positive non-endospore forming bacilli, Gram negative bacilli and others. Two groups were selected to continue the study: Gram negative bacilli since it was the most abundant, and Gram positive sporulated bacilli, seeking their sporulating capacity as an advantage for inoculants formulation. The ability of these to release siderophores and chitinases in vitro was evaluated. Ninety six isolates were siderophore producers, and 56 of them were also able to produce chitinases. Fifty percent of these were tested for growth promotion in tomato. The best results were obtained with 5 Gram negative bacilli and one Gram positive sporulated bacilli; 5 strains increased all growth parameters while one of them, N21.4, severely compromised plant growth. The ability of these 6 strains to induce systemic resistance against the leaf pathogen Xanthomonas campestris in tomato was evaluated. Five of them effectively reduced disease symptoms (up to 50%). The six strains were identified by 16s rDNA sequencing resulting in 3 Pseudomonas, 1 Bacillus and 2 Stenotrophomonas; it’s striking that 2 Pseudomonas protected up to 50% while the other increased disease incidence. This indicates that systemic induction is strain specific and not necessarily related to production of siderophores and chitinases.
KeywordsInduced systemic resistance Plant protection PGPR Priming Solanaceae Tomato
- Adesina MF, Lembke A, Costa R, Speksnijder A, Smalla K (2007) Screening of bacterial isolates from various European soils for in vitro antagonistic activity towards Rhizoctonia solani and Fusarium oxysporum: site-dependent composition and diversity revealed. Soil Biol Biochem 39:2818–2828CrossRefGoogle Scholar
- Bevivino A, Sarrocco S, Daimastri C, Tabacchioni S, Cantale C, Chiarinin L (1998) Characterization of a free-living maize-rhizosphere population of Burkholderia cepacia: effect of seed treatment on disease suppression and growth promotion of maize. FEMS Microbiol Ecol 27(3):225–237CrossRefGoogle Scholar
- Domenech J, Reddy MS, Kloepper JW, Ramos B, Gutierrez-Mañero FJ (2006) Combined application of the biological product LS213 with Bacillus, Pseudomonas or Chryseobacterium for growth promotion and biological control of soil-borne diseases in pepper and tomato. Biocontrol 51:245–258CrossRefGoogle Scholar
- Harmann JH (1967) Modern factor analysis, 2nd edn. Univ. Chicago Press, ChicagoGoogle Scholar
- Lorito M, Di Pietro A, Hayes CK, Woo SL, Harman GE (1993) Antifungal, synergistic interaction between chitinolytic enzymes from Thrichoderma harzianum and Enterobacter cloacae. Appl Environ Microbiol 83:721–728Google Scholar
- Lucas García JA, Probanza A, Ramos B, Ruiz Palomino M, Gutiérrez Mañero FJ (2004) Effect of inoculation of Bacillus licheniformis on tomato and pepper. Agric Environ (formerly Agronomie) 24:169–176Google Scholar
- Lynch JM (1990) The rhizosphere. Wiley-Interscience, ChichesterGoogle Scholar
- Marten P, Brueckner S, Berg G (2001) Biological plant protection using rhizobacteria—an environmental friendly alternative for biological control of soilborne and seedborne phytopathogenic fungi. Gesunde Pflanzen 53:224–234Google Scholar
- Ramos B, Garcia JAL, Probanza A, Doménech J, Gutierrez Mañero FJ (2003) Influence of an indigenous European alder (Alnus glutinosa L. Gaertn) rhizobacterium (Bacillus pumilus) on the growth of alder and its rhizosphere microbial community structure in two soils. New Forest 25:149–159CrossRefGoogle Scholar
- Ramos Solano B, Barriuso Maicas J, Gutiérrez Mañero FJ (2008a) Physiological and molecular mechanisms of PGPRs. In: Ahamd I, Pichtel J, Hayat S (eds) Plant-bacteria interaction: concepts and technologies for promoting plant growth. Wiley, New York, pp 41–54Google Scholar
- Ramos Solano B, Barriuso J, Pereyra MT, Domenech J, Gutierrez Mañero FJ (2008b) Systemic disease protection elicited by plant growth promoting rhizobacteria strains: relationship between metabolic responses, systemic disease protection and biotic elicitors. Phytopathol 98:451–457CrossRefGoogle Scholar