Phytoextraction of heavy metals by two Salicaceae clones in symbiosis with arbuscular mycorrhizal fungi during the second year of a field trial
- 604 Downloads
We evaluated the potential of Salix viminalis (5027) and Populus × generosa for the phytoextraction of heavy metals (HM) inoculated or not with an arbuscular mycorrhizal (AM) fungus Glomus intraradices during a second year of growth in a randomized complete block field trial on a slightly contaminated site. Both plant clones produced high aboveground biomass yields, however P. × generosa produced significantly more biomass than S. viminalis. The two plant clones accumulated high concentrations of Cd and Zn in their shoots, while Cu and Pb were stored in their roots. In general, S. viminalis accumulated higher concentrations of HM. The inoculation of G. intraradices in the previous year did not influence plant clones’ biomass yields during the second growing season. However, Cu and Cd translocation to shoots was limited, and Cu was preferentially concentrated in roots of inoculated plants, compared to non-inoculated plants, which were also colonized by native AM fungi taxa. Efficiency of S. viminalis and P. × generosa for Cd and Zn rehabilitation in slightly contaminated soil has been demonstrated, but mycorrhizal inoculation did not significantly increase HM extraction.
KeywordsHeavy metal Phytoextraction Salix viminalis Hybrid poplar Arbuscular mycorrhizal fungi
analysis of variance
biological concentration factor
cation exchange capacity
This project received financial support from the Federation of Canadian Municipalities’ (FCM) Green Municipal Fund (GMF), the South-West district of Montreal and the Montreal Centre of Excellence in Brownfields Rehabilitation (MCEBR). The authors would like to express their gratitude to Adela Voicu, Stéphane Daigle, Traian Ion Teodorescu, Hélène Lalande and Marie-Claude Turmel for their help with settings, samplings, analyses and statistics.
- Beaulien M, Drouin R (1999) Politique de protection des sols et de réhabilitation des terrains contaminés. Quebec Environment Ministry, Les Publications du Québec, Ste-Foy, p 120Google Scholar
- Gaur A, Adholeya A (2004) Prospects of arbuscular mycorrhizal fungi in phytoremediation of heavy metal contaminated soils. Curr Sci 86:528–534Google Scholar
- Hammer D, Kayser A, Keller C (2003) Phytoextraction of Cd and Zn with Salix viminalis in field trials. Soil Use Manag 19:187–192Google Scholar
- Hassan SE, Hijri M, St-Arnaud M (2009) PCR-DGGE analysis of arbuscular mycorrhizal fungi diversity in heavy metal polluted soils. In Proceedings of the 59th Ann Conf Can Soc Microbiol, Concordia University, Montreal, Canada. pp 69Google Scholar
- Kabata-Pendias A (2001) Trace elements in soils and plants. CRC, Bocca Raton, p 413Google Scholar
- Labrecque M, Lefebvre R, St-Arnaud M (2006) Growth potential and heavy metals accumulation in poplar and willow plants inoculated with an arbuscular mycorrhizal fungi. In Proceeding of the 27th annual meeting of the Society of Environmental Toxicology and Chemistry (SETAC). Montreal, Quebec, Canada, p 356Google Scholar
- Lefebvre R (2007) Réhabilitation d’un site contaminé de la ville de Montréal par des approches de phytoremédiation. MSc Thesis, Department of Biological Sciences, University of Montreal, Montreal, Canada. pp 102Google Scholar
- St-Arnaud M, Vujanovic V (2007) Effect of the arbuscular mycorrhizal symbiosis on plant diseases and pests. In: Hamel C, Plenchette C (eds) Mycorrhizae in crop production. Haworth Food & Agricultural Products Press, Binghampton, pp 67–122Google Scholar