Plant and Soil

, Volume 332, Issue 1–2, pp 55–67 | Cite as

Phytoextraction of heavy metals by two Salicaceae clones in symbiosis with arbuscular mycorrhizal fungi during the second year of a field trial

  • Laurence Bissonnette
  • Marc St-Arnaud
  • Michel LabrecqueEmail author
Regular Article


We evaluated the potential of Salix viminalis (5027) and Populus × generosa for the phytoextraction of heavy metals (HM) inoculated or not with an arbuscular mycorrhizal (AM) fungus Glomus intraradices during a second year of growth in a randomized complete block field trial on a slightly contaminated site. Both plant clones produced high aboveground biomass yields, however P. × generosa produced significantly more biomass than S. viminalis. The two plant clones accumulated high concentrations of Cd and Zn in their shoots, while Cu and Pb were stored in their roots. In general, S. viminalis accumulated higher concentrations of HM. The inoculation of G. intraradices in the previous year did not influence plant clones’ biomass yields during the second growing season. However, Cu and Cd translocation to shoots was limited, and Cu was preferentially concentrated in roots of inoculated plants, compared to non-inoculated plants, which were also colonized by native AM fungi taxa. Efficiency of S. viminalis and P. × generosa for Cd and Zn rehabilitation in slightly contaminated soil has been demonstrated, but mycorrhizal inoculation did not significantly increase HM extraction.


Heavy metal Phytoextraction Salix viminalis Hybrid poplar Arbuscular mycorrhizal fungi 



arbuscular mycorrhizal


analysis of variance


biological concentration factor


cation exchange capacity


heavy metal



This project received financial support from the Federation of Canadian Municipalities’ (FCM) Green Municipal Fund (GMF), the South-West district of Montreal and the Montreal Centre of Excellence in Brownfields Rehabilitation (MCEBR). The authors would like to express their gratitude to Adela Voicu, Stéphane Daigle, Traian Ion Teodorescu, Hélène Lalande and Marie-Claude Turmel for their help with settings, samplings, analyses and statistics.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Laurence Bissonnette
    • 1
  • Marc St-Arnaud
    • 1
  • Michel Labrecque
    • 1
    Email author
  1. 1.Institut de Recherche en Biologie VégétaleUniversité de Montréal and Jardin Botanique de MontréalMontrealCanada

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