Plant and Soil

, Volume 331, Issue 1–2, pp 361–375

Silicon enhances growth independent of silica deposition in a low-silica rice mutant, lsi1

  • Mami Isa
  • Shuqin Bai
  • Takushi Yokoyama
  • Jian Feng Ma
  • Yushi Ishibashi
  • Takashi Yuasa
  • Mari Iwaya-Inoue
Regular Article

Abstract

To examine whether silica bodies are essential for silicon-enhanced growth of rice seedlings, we investigated the response of rice, Oryza sativa L., to silicon treatment. Silicic acid treatment markedly enhanced the SPAD (soil plant analytical development) values of leaf blades and the growth and development of leaves and lateral roots in cvs. Hinohikari and Oochikara, and a low-silicon mutant, lsi1. Combination of ethanol–benzene displacement and staining with crystal violet lactone enabled more detailed histochemical analysis to visualize silica bodies in the epidermis under bright-field microscopy. Supply of silicon induced the development of motor cells and silica bodies in epidermal cells in Hinohikari and Oochikara but not or marginal in lsi1. X-ray analytical microscopy detected silicon specifically in the leaf sheath, the outermost part of the stem, and the leaf blade midrib, suggesting that silicon is distributed to tissues involved in maintaining rigidity of the plant to prevent lodging, rather than being passively deposited in growing tissues. Silicon supplied at high dose accumulated in all rice seedlings and enhanced growth and SPAD values with or without silica body formation. Silicon accumulated in the cell wall may play an important physiological role different from that played by the silica deposited in the motor cell and silica bodies.

Keywords

Cultivar EDX Electron microscopy Histochemical analysis lsi1 Motor cell Oryza sativa Rice Silica body X-ray analytical microscope 

Abbreviations

eV

Electron voltage

Si

Silicic acid

SPAD

Soil and plant analysis development

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Mami Isa
    • 1
  • Shuqin Bai
    • 2
  • Takushi Yokoyama
    • 2
  • Jian Feng Ma
    • 3
  • Yushi Ishibashi
    • 4
  • Takashi Yuasa
    • 5
  • Mari Iwaya-Inoue
    • 5
  1. 1.Graduate School of Bioresource and Bioenvironmental SciencesKyushu UniversityFukuokaJapan
  2. 2.Department of Chemistry, Faculty of ScienceKyushu UniversityFukuokaJapan
  3. 3.Research Institute for BioresourcesOkayama UniversityKurashikiJapan
  4. 4.Coastal Bioenvironment CenterSaga UniversityKaratsuJapan
  5. 5.Department of Plant Resources, Faculty of AgricultureKyushu UniversityFukuokaJapan

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