Evaluation of diverse soybean germplasm for root growth and architecture
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Root characteristics of soybean (Glycine max (L.) Merr.) improve drought avoidance by increasing water uptake from the soil profile. Screening genotypes for improved root architecture without breaking the taproots or losing lateral roots is a challenge. Due to difficulty in separating roots from field or potting soil, a rapid and effective screening method with a suitable growth medium to assess root characteristics under controlled conditions needs to be established. We describe two screening techniques “the cone system” and “the tube system” using turface:sand medium. In the cone system thirty four soybean lines including cultivars and exotic plant introduction (PI) lines were evaluated for tap root length and root biomass, 12 days after sowing. Eight replications per line were grown in a growth chamber. Significant differences among genotypes for tap root length were detected by the cone system. Validity of results from the cone system was tested by evaluating root growth 21 days after planting for eight lines in the tube system. A coefficient of determination of 0.72 indicated good agreement between the two screening systems for evaluating genotypes for rooting depth. The cone system will be a useful method to easily and rapidly assess soybean genotypes for root growth.
KeywordsSoybean Root growth Tap root length Plant introduction line
We thank Theresa Musket for assistance in this study. We also thank Prof. JE. Specht, University of Nebraska and Prof. Saghai-Maroof, Virginia Tech for providing the seed of mapping parental lines.
- Bohm W, Madukor H, Taylor HM (1977) Comparison of five methods for characterizing soybean rooting density. Agron J 69:415–419Google Scholar
- Busscher WJ, Frederick JR, Bauer PJ (2000) Timing effects of deep tillage on penetration resistance and wheat and soybean yield. Soil Sci Soc Amer J 64:999–1003Google Scholar
- Carter TE, Rufty TW (1993) A soybean plant introduction exhibiting drought and aluminum tolerance. In: Kuo G (ed) Adaptation of Vegetables and other Food Crops to Temperature and Water Stress. Asian Vegetable Research and Development Center, Publ, Tainan, Taiwan, pp 335–346Google Scholar
- Chaky JM, Specht JE, Cregan PB (2003). Advanced backcross QTL analysis in a mating between glycine max and glycine soja. Plant and Animal Genome Abstracts. P545Google Scholar
- Hirasawa T, Tanaka K, Miyamoto D, Takei M, Ishihara K (1994) Effects of pre-flowering moisture deficits on dry matter production and ecophysiological characteristics in soybean plants under drought conditions during grain filling. Jpn J Crop Sci 63:21–730Google Scholar
- Kaspar TC (1985) Growth and development of soybean root systems. In: Shibles R (ed) Proceedings World Soybean Research Conference III, Ames, IA. 12–17 Aug. 1984. Westview Press, Boulder, Colarado, pp 841–847Google Scholar
- Kiang YI, Chiang YC, Kaizuma N (1992) Genetic diversity in natural populations of wild soybean in Iwate prefecture. Japan J Hered 83:325–329Google Scholar
- Taylor HM, Burnett E, Booth GD (1978) Taproot elongation rates of soybeans. J Agron and Crop Sci 146:33–39Google Scholar
- Waisel Y, Eshel A, Kafkafi U (2002) Plant roots: The hidden half, 3rd edn. Marcel Dekker, New York, p 1120Google Scholar