Plant and Soil

, Volume 329, Issue 1–2, pp 75–89 | Cite as

Prospects and problems of simple linear models for estimating symbiotic N2 fixation by crop and pasture legumes

Regular Article

Abstract

Symbiotic dinitrogen (N2) fixation of crop and pasture legumes is a critical component of agricultural systems, but its measurement is expensive and labour intensive. Simple models which can provide approximations based on crop or pasture dry matter production would be useful for agrononomists and those interested in regional nitrogen (N) cycle fluxes. We investigate meta analysis of published data on legume shoot dry matter production, shoot %N and legume %N fixed (%Ndfa) and look for relationships among these, as a possible way of providing useful approximations of N2 fixation. We restricted our analysis to Australian studies where we have ready access to the primary data and where cultivars, management and climate are more constrained compared to a universal dataset. Regression analysis between shoot dry matter and amounts of shoot N2 fixed were strong for all crop and pasture legumes with significant differences in slope and intercept values being obtained between pastures and crops, and between chickpea (Cicer arietinium) and all other crop and pasture legumes. Annual pasture legumes showed the strongest linear relationship between N2 fixation and shoot dry matter and had the greatest slope (20.2–24.3 kg N2 fixed/t), compared to 18.7 kg N2 fixed/t for the perennial pasture legume lucerne (alfalfa, Medicago sativa), and between 10.7 to 23.0 kg N2/t for crop legumes, depending upon species. It was recognised that the use of such shoot-based relationships would underestimate the total amounts of N2 fixed since the contributions of fixed N present in, or derived from, roots and nodules are not included. Furthermore there needs to be careful consideration of the validity of an intercept term, which might reflect suppression of N2 fixation at low dry matter and high soil mineral N availability, or possibly the use of non-linear regression. For chickpea crops grown in north-eastern Australia, multiple regression indicated that N2 fixation was much more closely correlated with %Ndfa than dry matter production. Evidence presented also indicated that %Ndfa of other crops and lucerne in this region may similarly be influenced by soil mineral N. The regression approach presented provides a statistical basis to approximate N2 fixation in the first instance. This work highlights some of the dangers of fitting single regressions to aggregated datasets and using these to approximate symbiotic N2 fixation. The analysis indicates that where pasture legumes are grown in mixtures with non-legumes, and driven to high dependence on N2 fixation, simple linear regressions may be quite useful, provided that possible differences between species are investigated as the slopes of the regressions between these can be quite different. For crop legumes, where low dependence on N2 fixation can occur at higher mineral N availability, there is a need to carefully consider the intercept term, obtain estimates of mineral N availability, and/or resort to non-linear models. The gross generalisations presented in scatter plots cannot be reliably applied any more specifically, even within the datasets from which they were generated, and in some cases even within legume species between regions. They cannot substitute for direct measurement where any certainty is required under a particular set of defined conditions.

Keywords

Roots N fixation measurement Medicago Pea Bean Lupin Clover Legumes N inputs 

Supplementary material

11104_2009_136_MOESM1_ESM.doc (55 kb)
ESM1Sources of data used in the analysis (available as accessory material on request) (DOC 55 kb)

References

  1. Angus J, Good A (2004) Dryland cropping in Australia. In: Rao S, Ryan J (eds) Challenges and strategies for dryland agriculture. Crop Science Society of America and American Society of Agronomy, MadisonGoogle Scholar
  2. Bolger TP, Pate JS, Unkovich MJ, Turner NC (1995) Estimates of seasonal nitrogen fixation of annual subterranean clover-based pastures using the 15N natural abundance technique. Pl Soil 175:57–66CrossRefGoogle Scholar
  3. Bowman AM, Peoples MB, Smith W, Brockwell J (2002) Factors affecting nitrogen fixation by dryland lucerne in central-western New South Wales. Aust J Exptl Agric 42:439–451CrossRefGoogle Scholar
  4. Carlsson G, Huss-Danell K (2003) Nitrogen fixation in perennial forage legumes in the field. Pl Soil 253:353–372CrossRefGoogle Scholar
  5. Cocks PS, Mathison MJ, Crawford EJ (1980) From wild plants to pasture cultivars: Annual medics and subterranean clover in southern Australia. In: Summerfield RJ, Bunting AH (eds) Advances in legume science. Royal Botanic Gardens, Kew, pp 569–596Google Scholar
  6. Crawford M, Grace P, Oades M (1998) Effect of defoliation of medic pastures on below-ground carbon allocation and root production. In: Lal R, Kimble J, Follet R, Stewart B (eds) Management of carbon sequestration in soil. CRC Press, Boca Raton, pp 381–390Google Scholar
  7. Crews T, Peoples M (2004) Legume versus fertilizer sources of nitrogen: ecological tradeoffs and human needs. Agric Ecosyst Environ 102:279–297CrossRefGoogle Scholar
  8. Dalal R, Strong W, Doughton J, Weston E, McNamara G, Cooper J (1997) Sustaining productivity of a vertisol at Warra, Queensland, with fertilisers, no-tillage, or legumes 4. Nitrogen fixation, water use and yield of chickpea. Aust J Exptl Agric 37:667–676CrossRefGoogle Scholar
  9. Dear BS, Cocks PS, Peoples MB, Swan AD, Smith AB (1999) Nitrogen fixation by subterranean clover (Trifolium subterraneum L.) growing in pure culture and in mixtures with varying densities of lucerne (Medicago sativa L.) or phalaris (Phalaris aquatica L.). Aust J Agric Res 50:1047–1058CrossRefGoogle Scholar
  10. Evans J, O’Connor GE, Turner GL, Coventry DR, Fettel N, Mahoney J, Armstrong EL, Walsgott DN (1989) Nitrogen fixation and its value to soil N increase in lupin, field pea and other legumes in south-eastern Australia. Aust J Agric Res 40:791–805CrossRefGoogle Scholar
  11. Evans J, Fettell N, O’ Connor G, Carpenter D, Chalk P (1997) Effect of soil treatment with cereal straw and method of crop establishment on field pea (Pisum sativum L.) N2 fixation. Biol Fert Soils 24:87CrossRefGoogle Scholar
  12. Farrington P, Greenwood EZT, Trinick MJ, Smith DW (1977) Fixation, accumulation, and distribution of nitrogen in a crop of Lupinus angustifolius cv. Unicrop. Aust J Agric Res 28:237–248Google Scholar
  13. Frank DA, Kuns MM, Guido DR (2002) Consumer control of grasland and plant production. Ecology 83:602–606CrossRefGoogle Scholar
  14. Gladstones JS, Loneragan JF (1975) Nitrogen in temperate crop and pasture plants. Aust J Agric Res 26:103–112CrossRefGoogle Scholar
  15. Herridge DF, Marcellos H, Felton W, Turner G, Peoples M (1998) Chickpea in wheat based cropping systems of northern New South Wales III. Prediction of N2 fixation and N balance using soil nitrate at sowing and chickpea yield. Aust J Agric Res 49:409–418CrossRefGoogle Scholar
  16. Herridge DF, Peoples M, Boddey RM (2008) Global inputs of biological nitrogen fixation in agricultural systems. Pl Soil 311:1–18CrossRefGoogle Scholar
  17. Jensen ES (1987) Seasonal patterns of growth and nitrogen fixation in field-grown pea. Pl Soil 29–37Google Scholar
  18. Jensen ES, Hauggaard-Nielsen H (2003) How can increased use of biological N2 fixation in agriculture benefit the environment? Pl Soil 252:177–186CrossRefGoogle Scholar
  19. Khan DF, Peoples M, Chalk PM, Herridge D (2002) Quantifying below ground nitrogen of legumes. 2. A comparison of 15N and non-isotopic methods. Pl Soil 239:277–289CrossRefGoogle Scholar
  20. Khan DF, Peoples M, Schwenke G, Felton WL, Chen D, Herridge DF (2003) Effects of below-ground nitrogen on N balances of field-grown fababean, chickpea and barley. Aust J Agric Res 54:333–340CrossRefGoogle Scholar
  21. Mahieu S, Fustec J, Faure M-L, Corre-Hellou G, Crozat Y (2007) Comparison of two 15N labelling methods for assessing nitrogen rhizodeposition of pea. Pl Soil 295:193–205CrossRefGoogle Scholar
  22. Marcellos H, Felton W, Herridge D (1998) Chickpea in wheat based cropping systems of northern New South Wales I. N2 fixation and influence on soil nitrate and water. Aust J Agric Res 49:391–400CrossRefGoogle Scholar
  23. Maskey S, Bhattarai S, Peoples M, Herridge D (2001) On-farm measurements of nitrogen fixation by winter and summer legumes in the Hill and Terai regions of Nepal. Field Crops Res 70:209–221CrossRefGoogle Scholar
  24. Mayer J, Buegger F, Jensen ES, Schloter M, Heß J (2003) Estimating N rhizodeposition of grain legumes using a 15N in situ stem labelling method. Soil Biol Biochem 35:21–28CrossRefGoogle Scholar
  25. McCallum M, Peoples M, Connor D (2000) Contributions of nitrogen by field pea (Pisum sativum L.) in a continuous cropping sequence compared with lucerne (Medicago sativa L.) -based pasture ley in the Victorian Wimmera. Aust J Agric Res 51:13–22CrossRefGoogle Scholar
  26. McNeill A, Fillery I (2008) Field measurement of lupin belowground nitrogen accumulation and recovery in the subsequent cereal-soil system in a semi-arid Mediterranean-type climate. Pl Soil 302:297–316CrossRefGoogle Scholar
  27. McNeill AM, Zhu C, Fillery I (1997) Use of in situ 15 N-labelling to estimate the total below-ground nitrogen of pasture legumes in intact plant-soil systems. Aust J Agric Res 48:295–304CrossRefGoogle Scholar
  28. Nable RO, Bar-Akiva A, Loneragan JF (1984) Functional manganese requirement and its use as a critical value for diagnosis of manganese deficiency in subterranean clover ( Trifolium subterrraneum L. cv. Seaton Park). Ann Bot 54:39–49Google Scholar
  29. Nicholls N, Drosdowsky W, Lavery B (1997) Australian rainfall variability and change. Weather 52:66–71Google Scholar
  30. O’Connor GE, Evans J, Fettell NA, Bamforth I, Stuchberry J, Heenan DP, Chalk PM (1993) Sowing date and varietal effects on the N2 fixation of field pea and implications for improvement of soil nitrogen. Aust J Agric Res 44:151–163CrossRefGoogle Scholar
  31. Peoples M, Baldock J (2001) Nitrogen dynamics of pastures: nitrogen fixation inputs, the impact of legumes on soil nitrogen to Australian farming systems. Aust J Exptl Agric 41:327–346CrossRefGoogle Scholar
  32. Peoples MB, Bowman A, Gault R, Herridge D, McCallum M, McCormick K, Norton R, Rochester I, Scammell G, Schwenke G (2001) Factors regulating the contributions of fixed nitrogen by pasture and crop legumes to different farming systems of eastern Australia. Pl Soil 228:29–41CrossRefGoogle Scholar
  33. Peoples M, Brockwell J, Herridge D, Rochester I, Alves B, Boddey R, Dakora F, Bhattari S, Maskey S, Sampet C, Rerkesam B, Khan D, Hauggaard-Nielsen H, Jensen E (2009) Review article. The contributions of nitrogen-fixing crop legumes to the productivity of agricultural systems. Symbiosis 48:1–17CrossRefGoogle Scholar
  34. Perry MW (1989) Farming systems of southern Australia. In: Ayling GP (ed) 5th Australian agronomy conference. Australian Society of Agronomy, Perth, Western Australia, pp 167–180Google Scholar
  35. Pilbeam CJ, Wood M, Jones MJ (1997) Proportion of total and fixed nitrogen in shoots of lentil and chickpea grown in a Mediterranean-type environment. Exptl Agric 33:139CrossRefGoogle Scholar
  36. Robertson M, Carberry P, Huth N, Turpin J, Probert M, Poulton P, Bell M, Wright G, Yeates S, Brinsmead R (2002) Simulation of growth and development of diverse legume species in APSIM. Aust J Agric Res 53:429–446CrossRefGoogle Scholar
  37. Rochester I, Peoples M, Constable GA, Gault R (1998) Faba beans and other legumes add nitrogen to irrigated cotton cropping systems. Aust J Exptl Agric 38:253–260CrossRefGoogle Scholar
  38. Russell CA, Fillery IRP (1996) Estimates of lupin below-ground biomass N, dry matter and N turnover to wheat. Aust J Agric Res 47:1047–1059CrossRefGoogle Scholar
  39. Salvagiotti F, Cassman K, Specht J, Walters D, Weiss A, Dobermann A (2008) Nitrogen uptake, fixation and response to fertilizer N in soybeans: a review. Field Crops Res 108:1–13CrossRefGoogle Scholar
  40. Sanford P, Pate JS, Unkovich MJ (1994) A survey of proportional dependence of subterranean clover and other pasture legumes on N2 fixation in south-west Australia utilizing 15N natural abundance. Aust J Agric Res 45:165–181CrossRefGoogle Scholar
  41. Schwenke G, Peoples G, Turner G, Herridge D (1998) Does nitrogen fixation of commercial dryland chickpea and faba bean crops in north-west New South Wales maintain or enhance soil nitrogen? Aust J Exptl Agric 38:61–70CrossRefGoogle Scholar
  42. Sinclair T (1986) Water and nitrogen limitations in soybean grain production I. Model development. Field Crops Res 15:125–141CrossRefGoogle Scholar
  43. Statsoft Inc. (2004) Statistica. Statsoft Inc, TulsaGoogle Scholar
  44. Svejcar T, Christiansen S (1987) The influence of grazing pressure on rooting dynamics of Caucasian Bluestem. J Range Manage 40:224–227CrossRefGoogle Scholar
  45. Tsialtas JT, Kassioumi M, Veresoglou DS (2004) Seasonal changes of N2-fixation by Trifolium repens in an upland Mediterranean grassland. Eu J Agron 21:335–346CrossRefGoogle Scholar
  46. Turpin JE, Herridge DF, Robertson MJ (2002) Nitrogen fixation and soil nitrate interactions in field-grown chickpea and fababean. Aust J Soil Res 53:599–608Google Scholar
  47. Unkovich M, Pate J (2000) An appraisal of recent field measurements of symbiotic N2 fixation by annual legumes. Field Crops Res 211:211–228CrossRefGoogle Scholar
  48. Unkovich MJ, Pate JS, Hamblin MJ (1994) The nitrogen economy of broadacre lupin in southwest Australia. Aust J Agric Res 45:149–164CrossRefGoogle Scholar
  49. Unkovich MJ, Pate JS, Sanford P (1997) Nitrogen fixation by annual legumes in Australian Mediterranean agriculture. Aust J Agric Res 48:267–293CrossRefGoogle Scholar
  50. Unkovich MJ, Sanford P, Pate JS, Hyder M (1998) Effects of grazing on plant and soil nitrogen relations of pasture-crop rotations. Aust J Agric Res 49:475–485CrossRefGoogle Scholar
  51. Unkovich M, Herridge DF, Peoples MB, Boddey RM, Cadisch G, Giller K, Alves B, Chalk PM (2008) Measuring plant-associated nitrogen fixation in agricultural systems. ACIAR Monograph No.136. Australian Centre for International Agricultural Research, Canberra, p 258Google Scholar
  52. Walley F, Clayton G, Miller P, Carr P, Lafond G (2007) Nitrogen economy of pulse crop production in the Northern Great Plains. Agron J 99:1710–1718CrossRefGoogle Scholar
  53. Wichern F, Eberhardt E, Mayer J, Joergensen RG, Müller T (2008) Nitrogen rhizodeposition in agricultural crops: Methods, estimates and future prospects. Soil Biol Biochem 40:30–48CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Soil and Land Systems Group, Earth and Environmental SciencesThe University of AdelaideGlen OsmondAustralia
  2. 2.CSIRO Land and WaterGlen OsmondAustralia
  3. 3.CSIRO Plant IndustryCanberraAustralia

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