Plant and Soil

, Volume 320, Issue 1–2, pp 169–179 | Cite as

Responses of Pinus halepensis growth, soil microbial catabolic functions and phosphate-solubilizing bacteria after rock phosphate amendment and ectomycorrhizal inoculation

  • L. Ouahmane
  • J. C. Revel
  • M. Hafidi
  • J. Thioulouse
  • Y. Prin
  • A. Galiana
  • B. Dreyfus
  • R. DuponnoisEmail author
Regular Article


We examined the effects of an ectomycorrhizal (EM) fungus, Pisolithus sp., on of the growth of Pinus halepensis (Allepo pine) seedlings, soil microbial functions and rock phosphate solubilization in a un-disinfected soil amended or not with a Moroccan rock phosphate. Allepo pine seedlings were inoculated with an EM fungus (Pisolithus sp. strain PH4) isolated from a P. halepensis plantation and selected for its high ability to mobilize P from an inorganic form of phosphate. After 4 month’s culture in a disinfected substrate, plants were transferred in 10 l-containers filled with a natural forest soil and amended or not with rock phosphate powder. After 12 month’s culturing, the growth, needle nutrient concentrations of P. halepensis plants were measured. Soil microbial catabolic diversity was assessed by measuring CO2 production of substrate induced respiration responses. Fluorescent pseudomonads were isolated from each soil treatment and tested in axenic conditions for their ability to solubilize a source of inorganic phosphate. The results clearly showed that (i) P. halepensis growth was greatly promoted by the presence of the ectomycorrhizal fungus Pisolithus strain PH4 in a disinfected soil/vermiculite mixture and in a non disinfected soil, (ii) ectomycorrhizal inoculation induced significant changes in the functions of soil microbial communities and selected microorganisms potentially beneficial to the plant growth (i.e. phosphate-solubilizing fluorescent pseudomonad) and (iii) rock phosphate solubilisation was mainly dependent on EM inoculation and mycorrhizosphere microorganisms. These results were in accordance with previous studies where it was demonstrated that EM symbiosis has a beneficial effect on plant growth through a direct effect on the host plant but also an indirect effect via a selective pressure on soil microbiota that favours microorganisms potentially beneficial to plant growth.


Bacteria Ectomycorrhizosphere effect Fluorescent pseudomonads Pinus halepensis Pisolithus sp. Rock phosphate Soil functional abilities Morocco 



This work was funded by the Moroccan-France PRAD programme (PRAD 05/12). Programme de Recherche Agronomique pour le Développement) and by IRD (Institut de Recherche pour le Développement (Jeune Equipe Associée à l’IRD, JEAI « Usen »).


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • L. Ouahmane
    • 1
    • 2
  • J. C. Revel
    • 3
  • M. Hafidi
    • 1
  • J. Thioulouse
    • 4
  • Y. Prin
    • 5
  • A. Galiana
    • 5
  • B. Dreyfus
    • 6
  • R. Duponnois
    • 6
    • 7
    Email author
  1. 1.Laboratoire Ecologie et Environnement, Faculté des Sciences SemlaliaUniversité Cadi AyyadMarrakechMaroc
  2. 2.Direction Régionale des Eaux & Forêts du Haut AtlasMarrakechMaroc
  3. 3.Ecole Nationale Supérieure Agronomique de Toulouse, Castanet-Tolosan, Eco. Lab., UMR 5245, CNRS-UPS-INPTToulouseFrance
  4. 4.Laboratoire de Biométrie et Biologie Evolutive (UMR 5558); CNRS; Univ. Lyon 1Villeurbanne CedexFrance
  5. 5.CIRAD. UMR 113 CIRAD/INRA/IRD/SUP-AGRO/UM2. Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM). TA10/J, Campus International de BaillarguetMontpellierFrance
  6. 6.IRD. UMR 113 CIRAD/INRA/IRD/SUP-AGRO/UM2. Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM). TA10/J, Campus International de BaillarguetMontpellierFrance
  7. 7.IRD. Laboratoire Commun de Microbiologie IRD/ISRA/UCAD. Centre de Recherche de Bel AirDakarSénégal

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