Effect of soil type and soybean genotype on fungal community in soybean rhizosphere during reproductive growth stages
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Fungal communities in soybean rhizosphere from reproductive growth stages R1 (beginning bloom) to R8 (full maturity) were studied based on the polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) banding patterns of partial rDNA internal transcribed spacer regions (ITS1) and sequencing methods. Pot experiment subjecting three soybean genotypes grown in two soils (Mollisol and Alfisol) indicated that the soil type was the major factor in shaping the fungal communities in the soybean rhizosphere. Field experiment was conducted in an Alfisol field with three soybean genotypes, and both pot and field experiments showed that rhizosphere fungal communities shifted with growth stages, and more diversity of communities was found in early reproductive growth stages than later stages. No major difference among fungal communities of three soybean genotypes was detected at individual growth stage. BLAST search of ITS sequence data generated from excised DGGE bands showed that fungi belonging to Ascomycetes and Basidiomycetes predominantly inhabited in the soybean rhizosphere. In addition, a few bands had low similarity with database sequences inferred that unknown fungal groups existed in soybean rhizosphere.
KeywordsDGGE Fungal community ITS region Rhizosphere Soil type Soybean genotype
The authors are grateful to Professor Makoto Kimura, Nagoya University Japan, for his comments and critical review of this manuscript. This research was supported by grants from Science and Technology Bureau of Heilongjiang Province (GA06B101-3-1) and National Natural Science Foundation of China (40671099).
- Buchenauer H (1998) Biological control of soil-borne diseases by rhizobacteria. J Plant Dis Prot 105:329–348Google Scholar
- Cahyani VR, Matsuya K, Asakawa S, Kimura M (2004) Succession and phylogenetic profile of methanogenic archaeal communities during the composting process of rice straw estimated by PCR-DGGE analysis. Soil Sci Plant Nutr 50:555–563Google Scholar
- De Ridder-Duine AS, Kowalchuk GA, Gunnewiek PJAK, Smant W, van Veen JA, de Boer W (2005) Rhizosphere bacterial community composition in natural stands of Carex arenaria (sand sedge) is determined by bulk soil community composition. Soil Biol Biochem 37:349–357 doi:10.1016/j.soilbio.2004.08.005 CrossRefGoogle Scholar
- Duineveld B, Kowalchuck GA, Keijzer A, van Elsas JD, van Veen JA (2001) Analysis of bacterial communities in the rhizosphere of the chrysanthemum via denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA as well as DNA fragments coding for 16S rRNA. Appl Environ Microbiol 67:172–178 doi:10.1128/AEM.67.1.172-178.2001 PubMedCrossRefGoogle Scholar
- Hiltner L (1904) Uber neuere erfarungen und problem auf dem gebiet der bodenbakteriologie und unter besonderer berucksichtigung der grundung und brache. Arbeitent Dtsch Landwirtschafts-Gesellschaft 98:59–78Google Scholar
- Smalla K, Wieland G, Buchner A, Zock A, Parzy J, Kaiser S, Roskot N, Heuer H, Berg G (2001) Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: plant-dependent enrichment and seasonal shifts revealed. Appl Environ Microbiol 67:4742–4751 doi:10.1128/AEM.67.10.4742-4751.2001 PubMedCrossRefGoogle Scholar
- White TJ, Buns TD, Lee S, Taylor J (1990) Analysis of phylogenetic relationships by amplification and direct sequencing of ribosomal RNA genes. In: Innis MA, Gefland DH, Sninsky JJ, White TJ (eds) PCR protocols: A guide to methods and applications. Academic, New York, pp 315–322Google Scholar
- Xin H, Ma H (1987) Occurrence and control for soybean root rot. Soybean Sci 6:189–196 in ChineseGoogle Scholar