Plant and Soil

, Volume 312, Issue 1–2, pp 69–83 | Cite as

Root traits and taxonomic affiliation of nine herbaceous species grown in glasshouse conditions

  • Catherine Roumet
  • Françoise Lafont
  • Muhaymina Sari
  • Fernand Warembourg
  • Eric Garnier
Regular Article

Abstract

This study examines whether root traits differed between three major plant families (Asteraceae, Fabaceae and Poaceae) and whether they are related to root respiration and exudation. Nine traits related to biomass allocation, root topology, morphology, chemical composition and mycorrhizal colonisation were examined for nine C3 herbaceous species grown in controlled conditions. Poaceae differed from Fabaceae for the whole set of root traits examined except mycorrhizal colonisation, while Asteraceae showed intermediate characteristics. As compared to Fabaceae, Poaceae allocated more biomass to roots; showed a more sparsely branched root system with a small average root diameter, a high root dry matter content and a low nitrogen concentration. Root respiration was weakly related to root mass ratio and root dry matter content; no significant relationship was found between root functions and root architecture or morphology. This study shows that plant classification based on taxonomic affiliation reflects differences in root system traits and functions. Whole root system traits do not allow strong predictions of root respiration and exudation, perhaps because these processes are more linked to fine root than to whole root system traits.

Keywords

Functional groups Mycorrhization Rhizospheric processes Root respiration Root traits Taxonomic families 

References

  1. Anderson TM, Starmer WT, Thorne M (2007) Bimodal root diameter distributions in Serengeti grasses exhibit plasticity in response to defoliation and soil texture: implications for nutrient uptake. Funct Ecol 21:50–60Google Scholar
  2. Bonfante-Fasolo P (1984) Anatomy and morphology of VA mycorrhizae. In: Powell CL, Bagyaraj DJ (eds) VA mycorrhizas. CRC, Boca Raton, FL, USA, pp 5–33Google Scholar
  3. Bouma TJ, Nielsen KL, Van Hal J, Koutstaal B (2001) Root system topology and diameter distribution of species from habitats differing in inundation frequency. Funct Ecol 15:360–369CrossRefGoogle Scholar
  4. Bowen GD, Rovira AD (1991) The rhizosphere, the hidden half of the hidden half. In: Waisel Y, Kafkafi U (eds) Plant roots: the hidden half. Marcel Dekker, New York, pp 641–649Google Scholar
  5. Brundrett M (1991) Mycorrhizas in natural ecosystems. Adv Ecol Res 21:171–313CrossRefGoogle Scholar
  6. Canadell J, Jackson RB, Ehleringer JR, Mooney HA, Sala OE, Schulze ED (1996) Maximum rooting depth of vegetation at the global scale. Oecologia 108:583–595CrossRefGoogle Scholar
  7. Chapin FS (1980) The mineral nutrition of wild plants. Annu Rev Ecol Syst 11:233–260CrossRefGoogle Scholar
  8. Čiamporová M, Dekánková K, Ovečka M (1998) Intra- and interspecific variation in root length, root turnover and the underlying parameters. In: Lambers H, Poorter H Van Vuuren M (eds) Inherent variation in plant growth. Physiological mechanisms and ecological consequences. Backhuys, Leiden, pp 57–69Google Scholar
  9. Cohen J (1988) Statistical power analysis for the behavorial sciences, 2nd edn. Lawrence Erlbaum, Hillsdale, New JerseyGoogle Scholar
  10. Comas LH, Bouma TJ, Eissenstat DM (2002) Linking root traits to potential growth rate in six temperate tree species. Oecologia 132:34–43CrossRefGoogle Scholar
  11. Craine JM, Berin DM, Reich PB, Tilman DG, Knops JMH (1999) Measurement of leaf longevity of 14 species of grasses and forbs using a novel approach. New Phytol 142:475–481CrossRefGoogle Scholar
  12. Craine JM, Froehle J, Tilman DG, Wedin DA, Chapin FS (2001) The relationships among root and leaf traits of 76 grassland species and relative abundance along fertility and disturbance gradients. Oikos 93:274–285CrossRefGoogle Scholar
  13. Craine JM, Tilman D, Wedin D, Reich P, Tjoelker M, Knops J (2002a) Functional traits, productivity and effects on nitrogen cycling of 33 grassland species. Funct Ecol 16:563–574CrossRefGoogle Scholar
  14. Craine JM, Wedin D, Chapin FS III, Reich PB (2002b) Relationship between the structure of root systems and resource use for 11 North American grassland plants. Plant Ecol 165:85–100CrossRefGoogle Scholar
  15. del Pozo A, Garnier E, Aronson J (2000) Contrasted nitrogen utilization of annual C3 grass and legume crops: physiological explorations and ecological consequences. Acta Oecol 21:79–89CrossRefGoogle Scholar
  16. Díaz S, Hodgson J, Thompson K, Cabido M, Cornelissen J, Jalili A, Montserrat-Marti G, Grime J, Zarrinkamar F, Asri Y, Band S, Basconcelo S, Castro-Díez P, Funes G, Hamzehee B, Khoshnevi M, Pérez-Harguindeguy N, Pérez-Rontome M, Shirvany F, Vendramini F, Yazdani S, Abbas-Azimi R, Bogaard A, Boustani S, Charles M, Dehghan M, de Torres-Espuny L, Falczuk V, Guerrero-Campo J, Hynd A, Jones G, Kowsary E, Kazemi-Saeed F, Maestro-Martínez M, Romo-Diez A, Shaw S, Siavash B, Villar-Salvador P, Zak M (2004) The plant traits that drive ecosystems: evidence from three continents. J Veg Sci 15:295–304CrossRefGoogle Scholar
  17. Eissenstat DM (1992) Costs and benefits of constructing roots of small diameter. J Plant Nutr 15:763–782CrossRefGoogle Scholar
  18. Eissenstat DM (2000) Root structure and function in an ecological context. New Phytol 148:353–354CrossRefGoogle Scholar
  19. Eissenstat DM, Yanai RD (1997) The ecology of root lifespan. Adv Ecol Res 27:1–60CrossRefGoogle Scholar
  20. Falster DS, Warton DI and Wright IJ (2006) User’s guide to SMATR: standardised major axis tests and routines, version 2. http://www.bio.mq.edu.au:ecology:SMATR:SMATR_users_guide.pdf
  21. Faul F, Erdfelder E, Lang A-G, Buchner A (2007) G*Power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Meth 39:175–191Google Scholar
  22. Fitter A (1985) Functional significance of root morphology and root system architecture. In: Fitter AH, Atkinson D, Read DJ, Usher MB (eds) Ecological interactions in soil. Blackwell Scientific, Oxford, pp 87–106Google Scholar
  23. Fitter A, Nichols R, Harvey M (1988) Root system architecture in relation to life history and nutrient supply. Funct Ecol 2:345–351CrossRefGoogle Scholar
  24. Fitter AH, Stickland TR (1991) Architectural analysis of plant root systems. II, Influence of nutrient supply on architecture in contrasting plant species. New Phytol 118:383–389CrossRefGoogle Scholar
  25. Foster TE, Brooks JR (2005) Functional groups based on leaf physiology: are they spatially and temporally robust? Oecologia 144:337–352PubMedCrossRefGoogle Scholar
  26. Fransen B, de Kroon H, Berendse F (1998) Root morphological plasticity and nutrient acquisition of perennial grass species from habitats of different nutrient availability. Oecologia 115:351–358CrossRefGoogle Scholar
  27. Garnier E, Laurent G, Bellmann A, Debain S, Berthelier P, Ducout B, Roumet C, Navas M-L (2001) Consistency of species ranking based on functional leaf traits. New Phytol 152:69–83CrossRefGoogle Scholar
  28. Hector A, Schmid B, Beierkuhnlein C, Caldeira M, Diemer M, Dimitrakopoulos P, Finn J, Freitas H, Giller P, Good J, Harris R, Hogberg P, Huss-Danell K, Joshi J, Jumpponen A, Körner C, Leadley P, Loreau M, Minns A, Mulder C, O’Donovan G, Otway S, Pereira J, Prinz A, Read D, Scherer-Lorenzen M, Schulze E, Siamantziouras A, Spehn E, Terry A, Troumbis A, Woodward F, Yachi S, Lawton J (1999) Plant diversity and productivity experiments in European grasslands. Science 286:1123–1127PubMedCrossRefGoogle Scholar
  29. Hewitt EJ (1966) Sand and water culture methods used in the study of plant nutrition. Farnham Royal, CA BureauxGoogle Scholar
  30. Hodge A (2004) The plastic plant: root responses to heterogeneous supplies of nutrients. New Phytol 162:9–24CrossRefGoogle Scholar
  31. Hooper DU, Vitousek PM (1997) The effects of plant composition and diversity on ecosystem processes. Science 277:1302–1305CrossRefGoogle Scholar
  32. Hummel I, Vile D, Violle C, Devaux J, Ricci B, Blanchard A, Garnier E, Roumet C (2007) Relating root structure and anatomy to whole plant functioning in 14 herbaceous Mediterranean species. New Phytol 173:313–321PubMedCrossRefGoogle Scholar
  33. Koske RE, Gemma JN (1989) A modified procedure for staining roots to detect VA mycorrhizas. Mycol Res 92:486–505Google Scholar
  34. Lambers H, Poorter H (1992) Inherent variation in growth rate between higher plants: a search for physiological causes and ecological consequences. Adv Ecol Res 23:187–261CrossRefGoogle Scholar
  35. Lavorel S, Díaz S, Cornelissen JHC, Garnier E, Harrison SP, McIntyre S, Pausas JG, Pérez-Harguindeguy N, Roumet C, Urcelay C (2007) Plant functional types: are we getting any closer to the Holy Grail? In: Canadell JG, Pataki D, Pitelka L (eds) Terrestrial ecosystems in a changing world, the IGBP series. Springer, Berlin, pp 149–160CrossRefGoogle Scholar
  36. Morrison LW (2007) Assessing the reliability of ecological monitoring data: power analysis and alternative approaches. Nat Areas J 27:83–91CrossRefGoogle Scholar
  37. Newsham KK, Fitter AH, Watkinson AR (1995) Multi-functionality and biodiversity in arbuscular mycorrhizas. Trends Ecol Evol 10:407–411CrossRefGoogle Scholar
  38. Philipps JM, Hayman DS (1970) Improved procedures for clearing and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Trans Br Mycol Soc 55:158–161CrossRefGoogle Scholar
  39. Pregitzer KS, DeForest JL, Burton AJ, Allen MF, Ruess RW, Hendrick RL (2002) Fine root architecture of nine north American trees. Ecol Monogr 72:293–309CrossRefGoogle Scholar
  40. Pregitzer KS, Laskowski MJ, Burton AJ, Lessard VC, Zak DR (1998) Variation in sugar maple root respiration with root diameter and soil depth. Tree Physiol 18:665–670PubMedGoogle Scholar
  41. Reich PB, Buschena C, Tjoelker MG, Wrage M, Knops J, Tilman D, Machado JL (2003) Variation in growth rate and ecophysiology among 34 grassland and savanna species under contrasting N supply: a test of functional group differences. New Phytol 157:617–631CrossRefGoogle Scholar
  42. Reich PB, Walters MB, Tjoelker MG, Vanderklein DW, Buschena C (1998) Photosynthesis and respiration rates depend on, leaf and root morphology in nine boreal tree species differing in relative growth rate. Funct Ecol 12:395–405CrossRefGoogle Scholar
  43. Reynolds HL, D’Antonio C (1996) The ecological significance of plasticity in root weight ratio in response to nitrogen: opinion. Plant Soil 185:75–97CrossRefGoogle Scholar
  44. Robinson D, Rorison IH (1988) Plasticity in grass species in relation to nitrogen supply. Funct Ecol 2:249–257CrossRefGoogle Scholar
  45. Roumet C, Urcelay C, Díaz S (2006) Suites of root traits differ between annual and perennial species growing in the field. New Phytol 170:357–368PubMedCrossRefGoogle Scholar
  46. Ryser P (1996) The importance of tissue density for growth and life span of leaves and roots: a comparison of five ecologically contrasting grasses. Funct Ecol 10:717–723CrossRefGoogle Scholar
  47. Ryser P (2006) The mysterious root length. Plant Soil 286:1–6CrossRefGoogle Scholar
  48. Šmilauerová M, Šmilauer P (2007) What youngsters say about adults: seedling roots reflect clonal traits of adult plants. J Ecol 95:406–413CrossRefGoogle Scholar
  49. Tarafdar NC, Marschner H (1994) Phosphatase activity in the rhizosphère and hydrosphere of VA mycorrhizal wheat supplied with inorganic and organic phosphorus. Soil Biol Biochem 26:387–395CrossRefGoogle Scholar
  50. Taub DR, Goldberg D (1996) Root system topology of plants from habitats differing in soil resource availability. Funct Ecol 10:258–264CrossRefGoogle Scholar
  51. Thomas L (1997) Retrospective power analysis. Conserv Biol 11:276–280CrossRefGoogle Scholar
  52. Tilman D, Reich P, Knops J, Wedin D, Mielke T, Lehman C (2001) Diversity and productivity in a long-term grassland experiment. Science 294:843–845PubMedCrossRefGoogle Scholar
  53. Tjoelker MG, Craine JM, Wedin D, Reich PB, Tilman D (2005) Linking leaf and root trait syndromes among 39 grassland and savannah species. New Phytol 167:493–508PubMedCrossRefGoogle Scholar
  54. Tutin TG, Burges NA, Chater AO, Edmondson JR, Heywood WH, Moore DM, Valentine DH, Walters SM, Webb DA (1968–1980) Flora Europea, vol. 2–5. 1st edn. Cambridge University Press, CambridgeGoogle Scholar
  55. Wahl S, Ryser P (2000) Root tissue structure is linked to ecological strategies of grasses. New Phytol 148:459–471CrossRefGoogle Scholar
  56. Warembourg FR, Roumet C, Lafont F (2003) Differences in rhizosphere carbon-partitioning among plant species of different families. Plant Soil 256:347–357CrossRefGoogle Scholar
  57. Warton DI, Wright IJ, Falster DS, Westoby M (2006) Bivariate line-fitting methods for allometry. Biol Rev 81:259–291PubMedCrossRefGoogle Scholar
  58. Weiner J, Martinez S, Müller-Schärer H, Stoll P, Schmid B (1997) How important are environmental maternal effects in plants? A study with Centaurea maculosa. J Ecol 85:133–142CrossRefGoogle Scholar
  59. Werner C, Smart JS (1973) Some new methods of topologic classification of channel networks. Geogr Anal 5:271–295Google Scholar
  60. Wright IJ, Reich PB, Cornelissen JHC, Falster DS, Garnier E, Hikosaka K, Lamont BB, Lee W, Oleksyn J, Osada N, Poorter H, Villar R, Warton DI, Westoby M (2005) Assessing the generality of global leaf trait relationships. New Phytol 166:485–496PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Catherine Roumet
    • 1
  • Françoise Lafont
    • 1
  • Muhaymina Sari
    • 2
  • Fernand Warembourg
    • 1
  • Eric Garnier
    • 1
  1. 1.CNRS, Centre d’Ecologie Fonctionnelle et EvolutiveMontpellier Cedex 5France
  2. 2.Department de BiologieUniversité de SherbrookeSherbrookeCanada

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