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Plant and Soil

, Volume 302, Issue 1–2, pp 53–69 | Cite as

Management of soil microbial communities to enhance populations of Fusarium graminearum-antagonists in soil

  • Carlos Perez
  • Ruth Dill-MackyEmail author
  • Linda L. Kinkel
Regular Article

Abstract

Fusarium head blight (FHB), incited by Fusarium graminearum Schwabe is one of the most devastating diseases of wheat. Primary inoculum generated on crop residue is the driving force of FHB epidemics. Fusarium survival on crop residues is affected by soil microbial antagonists. The incorporation of green manures has been shown to increase the density and diversity of microbes in soils, particularly the density and the pathogen-inhibitory activity of specific bacteria and fungi. Evidence of increased streptomycete populations in soil as a response to green manure incorporation, and their negative effect on the survival of Fusarium oxysporum Schlechtendahl in soil, suggests their potential use to reduce the survival of related pathogens. There is, however, no precedent for the use of green manures to promote indigenous streptomycete populations to control FHB. This study investigated the use of green manures (sorghum–sudangrass hybrid [Sorghum bicolor (L.) Moench–S. bicolor (L.) Moench var. sudanense (Piper)] and common buckwheat [Fagopyrum esculentum (Moench)]) for reducing F. graminearum survival in association with wheat residues. Soil bacterial density, streptomycete density and the density and inhibitory activity of F. graminearum-antagonists were monitored from planting until 3 and 6 months following the incorporation of green manures in greenhouse and field experiments, respectively. The decomposition of wheat residues and survival of Fusarium in residues was also assessed. The use of green manures did not statistically impact the survival of F. graminearum in wheat residue. However, green manures promoted the development of higher densities and antagonistic abilities of F. graminearum-antagonists in soils. Additionally, streptomycete densities and F. graminearum-antagonist densities were significantly and positively correlated with reduced survival of Fusarium. The results of our study suggest that the use of green manures can enhance populations of indigenous soil microorganisms antagonistic to the survival of F. graminearum in wheat residue.

Keywords

Antagonism Biocontrol Crop residues Fusarium head blight Green manures Streptomycetes 

Abbreviations

FHB

Fusarium head blight

WA

Water agar

SCA

Starch casein agar

OA

Oatmeal agar

CFU

Colony-forming units

PDWA

Potato dextrose water agar

PDA

Potato dextrose agar

KMA

Komada’s medium agar

CLA

Carnation leaf-piece agar

C:N

Carbon:nitrogen ratio

Notes

Acknowledgements

This project was partially funded by the United States Department of Agriculture (USDA), North Central Region (NCR) Sustainable Agriculture Research and Education (SARE) program, project GNC05-054. The authors wish to thank Kun Xiao, Jennifer Flor, Dale Johnson, Amber Lamoureux, C. Kent Evans, Bacilio Salas, Amar Elakkad, Karen Wennberg, and Mario Carrillo for their technical assistance in this research.

References

  1. Abawi G, Widmer T (2000) Impact of soil health management practices on soilborne pathogens, nematodes and root diseases of vegetable crops. Appl Soil Ecol 15:37–47CrossRefGoogle Scholar
  2. Abdallahi M, N’Dayegamiye A (2000) Effects of green manures on soil physical and biological properties and on wheat yields and nitrogen uptake. Can J Soil Sci 80:81–89Google Scholar
  3. Alabouvette C, Hoper H, Lemanceau P, Steinberg C (1996) Soil suppressiveness to diseases induced by soil-borne plant pathogens. In: Stotzky G, Bollag J (eds) Soil biochemistry. Marcel Dekker, New York, pp 371–413Google Scholar
  4. Bai G, Shaner G (1994) Scab of wheat: prospects for control. Plant Dis 78:760–766CrossRefGoogle Scholar
  5. Bailey K, Lazarovits G (2003) Suppressing soil-borne diseases with residue management and organic amendments. Soil Tillage Res 72:169–180CrossRefGoogle Scholar
  6. Blackshaw R, Larney F, Lindwall C, Watson P, Derksen D (2001) Tillage intensity and crop rotation affect weed community dynamics in a winter wheat cropping system. Can J Plant Sci 81:805–813Google Scholar
  7. Blevins R, Frye W, Wagger M, Tyler D (1994) Residue management strategies for the southeast. In: Hatfield J, Stewart B (eds) Crop residue management: advances in soil science. CRC, Boca Raton, pp 63–76Google Scholar
  8. Bossio D, Scow K, Gunapala N, Graham K (1998) Determinants of soil microbial communities: effects of agricultural management, season, and soil type on phospholipid fatty acid profiles. Microb Ecol 36:1–12PubMedCrossRefGoogle Scholar
  9. Bulluck L, Ristaino J (2002) Effect of synthetic and organic soil fertility amendments on southern blight, soil microbial communities, and yield of processing tomatoes. Phytopathology 92:181–189CrossRefPubMedGoogle Scholar
  10. Burgess L (1981) General ecology of Fusaria. In: Nelson P, Tousson T, Cook R (eds) Fusarium: Diseases, biology and taxonomy. The Pennsylvania State University Press, University Park, pp 225–235Google Scholar
  11. Chamberlain K, Crawford D (1999) In vitro and vivo antagonism of pathogenic turfgrass fungi by Streptomyces hygroscopius strains YCED9 and WYE53. J Ind Microbiol Biotech 23:641–646CrossRefGoogle Scholar
  12. Champeil A, Dore T, Fourbet J (2004) Fusarium head blight: epidemiological origin of the effects of cultural practices on head blight attacks and the production of mycotoxins by Fusarium in wheat grains. Plant Sci 166:1389–1415CrossRefGoogle Scholar
  13. Davis J, Huisman O, Westerman D, Sorensen L, Schneider A, Stark J (1994) The influence of cover crops on the suppression of Verticillium wilt of potato. In: Zehnder G, Powelson M, Jannson R, Ramay K (eds) Advances in potato pest biology and management. The American Phytopathological Society, Saint Paul, pp 332–341Google Scholar
  14. DeGroot M (1975) Probability and statistics. Addison-Wesley, Reading, pp 723Google Scholar
  15. Dill-Macky R (1996) Fusarium head blight: recent epidemics and research efforts in the upper Midwest of the United States. In: Dubin H, Gilchrist L, Reeves J, McNab A (eds) Fusarium head scab: global status and future prospects. CIMMYT. Mexico, DF, pp 1–6Google Scholar
  16. Dill-Macky R, Jones R (2000) The effect of previous crop residues and tillage on Fusarium head blight of wheat. Plant Dis 84:71–76CrossRefGoogle Scholar
  17. Ehaliotis C, Cadisch G, Giller K (1998) Substrate amendments can alter microbial dynamics and nitrogen availability from maize residues to subsequent crops. Soil Biol Biochem 30:1281–1292CrossRefGoogle Scholar
  18. Evans C, Xie W, Dill-Macky R, Mirocha C (2000) Biosynthesis of deoxynivalenol in spikelets of barley inoculated with macroconidia of Fusarium graminearum. Plant Dis 84:654–660CrossRefGoogle Scholar
  19. Fisher N, Burgess L, Tousson T, Nelson P (1982) Carnation leaves as a substrate for preserving cultures of Fusarium species. Phytopathology 72:151–153CrossRefGoogle Scholar
  20. Garrett S (1970) Pathogenic root-infecting fungi. Cambridge University Press, Cambridge, pp 294Google Scholar
  21. Govaerts B, Mezzalama M, Sayre K, Crossa J, Nicol J, Deckers J (2006) Long-term consequences of tillage, residue management and crop rotation on maize/wheat root rot and nematode populations on subtropical highlands. Appl Soil Ecol 32:305–315CrossRefGoogle Scholar
  22. Herr L (1959) A method of assaying soils for numbers of actinomycetes antagonistic to fungal pathogens. Phytopathology 49:270–273Google Scholar
  23. Hoitink H, Boehm M (1999) Biocontrol within the context of soil microbial communities: a substrate-dependent phenomenon. Ann Rev Phytopathol 37:427–446CrossRefGoogle Scholar
  24. Janzen H, Kucey R (1988) Carbon, nitrogen and sulfur mineralization of residues as influenced by crop species and nutrient regime. Plant Soil 106:35–41CrossRefGoogle Scholar
  25. Jones C, Samac D (1996) Biological control of fungi causing alfalfa seedling damping-off with a disease-suppressive strain of Streptomyces. Biol Control 7:196–204CrossRefGoogle Scholar
  26. Khonga E, Sutton J (1988) Inoculum production and survival of Gibberella zeae in maize and wheat residues. Can J Plant Pathol 10:232–239CrossRefGoogle Scholar
  27. Kirkegaard J, Simpfendorfer S, Holland J, Bambach R, Moore K, Rebetzke G (2004) Effect of previous crops on crown rot and yield of durum and bread wheat in northern NSW. Aust J Agric Res 55:321–334CrossRefGoogle Scholar
  28. Komada H (1975) Development of a selective medium for quantitative isolation of Fusarium oxysporum from natural soil. Rev Plant Protect Res 8:114–125Google Scholar
  29. Lazarovits G, Conn K, Potter J (1999) Reduction of potato scab, Verticillium wilt, and nematodes by soymeal and meat and bone meal in two Ontario potato fields. Can J Plant Pathol 21:345–353CrossRefGoogle Scholar
  30. Lockwood J (1988) Evolution of concepts associated with soilborne plant pathogens. Ann Rev Phytopathol 26:93–121CrossRefGoogle Scholar
  31. Lowry R (2006) Binomial probabilities. http://faculty.vassar.edu/lowry/binomialX.html. Cited 16 June 2007.
  32. Lupwayi N, Rice W, Clayton G (1998) Soil microbial diversity and community structure under wheat as influenced by tillage and crop rotation. Soil Biol Biochem 30:1733–1741CrossRefGoogle Scholar
  33. Luz W da, Stockwell C, Bergstrom G (2003) Biocontrol of Fusarium graminearum. In: Leonard K and Bushnell W (eds) Fusarium head blight of wheat and barley. APS, Saint Paul, pp 381–394Google Scholar
  34. Marschner P, Yang C, Lieberei R, Crowey D (2001) Soil and plant specific effects on bacterial community composition in the rhizosphere. Soil Biol Biochem 33:1437–1445CrossRefGoogle Scholar
  35. Mazzola M (2004) Assessment and management of soil microbial community structure for disease suppression. Ann Rev Phytopathol 42:35–59CrossRefGoogle Scholar
  36. Mazzola M, Granatstein D, Elving D, Mullinix K (2001) Suppression of specific apple root pathogens by Brassica napus seed meal amendment regardless of glucosinolate content. Phytopathology 91:673–679CrossRefPubMedGoogle Scholar
  37. McMullen M (2005) An update on the impact of Fusarium head blight on North American agriculture (Abstr). Phytopathology 95:S123Google Scholar
  38. McMullen M, Jones R, Gallenberg D (1997) Scab of wheat and barley: an emerging disease of devastating impact. Plant Dis 81:1340–1348CrossRefGoogle Scholar
  39. Ocamb C (1991) Ecology of soilborne Fusarium species associated with roots and the rhizosphere of Zea mays. Ph.D. Thesis, University of Minnesota, Saint Paul, pp 94Google Scholar
  40. Oehlert G (2000) A first course in design and analysis of experiments. Freeman, New York, pp 600Google Scholar
  41. Parr J, Papendick R (1978) Factors affecting the decomposition of crop residues by microorganisms. In: Crop residue management systems. Oschwald W. ASA Special Publ. 31. Madison, pp 101–129Google Scholar
  42. Pereyra S (2000) Survival and inoculum production of Gibberella zeae (Schwein.) Petch in wheat residue. M.S. Thesis, University of Minnesota, Saint Paul, pp 106Google Scholar
  43. Pereyra S, Dill-Macky R (2004) Survival and inoculum production of Gibberella zeae in wheat residue. Plant Dis 88:724–730CrossRefGoogle Scholar
  44. Peters R, Sturz A, Carter M, Sanderson J (2003) Developing disease-suppressive soils through crop rotation and tillage management practices. Soil Tillage Res 72:181–192CrossRefGoogle Scholar
  45. Pirgozliev S, Edwards S, Hare M, Jenkinson P (2003) Strategies for the control of Fusarium head blight in cereals. Eur J Plant Pathol 109:731–742CrossRefGoogle Scholar
  46. Reis E (1988) Doencas do trigo III. Gibberella. 2da edicao. (Wheat diseases III: Fusarium head blight, 2nd edn.) Sao Paulo, pp 13Google Scholar
  47. Shaner G (2003) Epidemiology of Fusarium head blight of small grain cereals in North America. In: Leonard K, Bushnell W (eds) Fusarium head blight of wheat and barley. APS, Saint Paul, pp 84–119Google Scholar
  48. Sturz A, Carter M, Johnston H (1997) A review of plant disease, pathogen interaction and microbial antagonism under conservation tillage in temperate humid agriculture. Soil Tillage Res 41:169–189CrossRefGoogle Scholar
  49. Sutton J (1982) Epidemiology of wheat head blight and maize ear rot caused by Fusarium graminearum. Can J Plant Pathol 4:175–209Google Scholar
  50. Sutton J, Vyn T (1990) Crop sequences and tillage practices in relation to diseases of winter wheat in Ontario. Can J Plant Pathol 12:358–368CrossRefGoogle Scholar
  51. Weller D, Raaijmakers J, McSpadden B, Tomashow L (2002) Microbial populations responsible for specific soil suppressiveness to plant pathogens. Ann Rev Phytopathol 40:309–348CrossRefGoogle Scholar
  52. Westover K, Kennedy A, Kelley S (1997) Patterns of rhizosphere microbial community structure associated with co-occurring plant species. J Ecol 85:863–873CrossRefGoogle Scholar
  53. Wiese M (1987) Compendium of wheat diseases, 2nd edn. APS, Saint Paul, pp 106Google Scholar
  54. Wiggins E (2003) Green manures and cropping sequences influence indigenous soil-borne antagonists and plant disease. M.S. Thesis, University of Minnesota, Saint Paul, pp 92Google Scholar
  55. Wiggins B, Kinkel L (2005a) Green manures and crop sequences influence potato diseases and pathogen inhibitory activity of indigenous streptomycetes. Phytopathology 95:178–185CrossRefGoogle Scholar
  56. Wiggins B, Kinkel L (2005b) Green manures and crop sequences influence alfalfa root rot and pathogen inhibitory activity among soil-borne streptomycetes. Plant Soil 268:271–283CrossRefGoogle Scholar
  57. Wilcoxson R, Kommedahl T, Ozmon E, Windels C (1988) Occurrence of Fusarium species in scabby wheat from Minnesota and their pathogenicity to wheat. Phytopathology 78:586–589CrossRefGoogle Scholar
  58. Windels C (2000) Economic and social impacts of Fusarium head blight: changing farms and rural communities in the northern great plains. Phytopathology 90:17–21CrossRefPubMedGoogle Scholar
  59. Wong L, Tekauz A, Leslie D, Abramson D, MacKenzie R (1992) Prevalence, distribution, and importance of Fusarium head blight in wheat in Manitoba. Can J Plant Pathol 14:233–238CrossRefGoogle Scholar
  60. Workneh F, van Bruggen A (1994) Suppression of corky root of tomatoes in organically managed soil associated with soil microbial activity and nitrogen status of soil and tomato tissue. Phytopathology 84:688–694CrossRefGoogle Scholar
  61. Xiao K, Kinkel L, Samac D (2002) Biological control of Phytophthora root rots on alfalfa and soybean with Streptomyces. Biol Control 23:285–295CrossRefGoogle Scholar
  62. Zadoks J, Chang T, Konzak C (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421CrossRefGoogle Scholar
  63. Zaitlin B, Turkington K, Parkinson D, Clayton G (2004) Effects of tillage and organic fertilizers on culturable soil actinomycete communities and inhibition of fungi by specific actinomycetes. Appl Soil Ecol 26:53–62CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Carlos Perez
    • 1
    • 2
  • Ruth Dill-Macky
    • 1
    Email author
  • Linda L. Kinkel
    • 1
  1. 1.Department of Plant PathologyUniversity of MinnesotaSt. PaulUSA
  2. 2.Departamento de Protección VegetalUniversidad de la RepúblicaPaysanduUruguay

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