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Plant and Soil

, Volume 301, Issue 1–2, pp 65–76 | Cite as

Shift in soil–plant nitrogen dynamics of an alpine–nival ecotone

  • Edith Huber
  • Wolfgang Wanek
  • Michael Gottfried
  • Harald Pauli
  • Peter Schweiger
  • Stefan K. Arndt
  • Karl Reiter
  • Andreas Richter
Regular Article

Abstract

We investigated the nitrogen (N) dynamics of an alpine–nival ecotone on Mt. Schrankogel, Tyrol, Austria, in relation to temperature. Natural abundance of 15N was used as a tool to elucidate differences in N cycling along an altitudinal transect ranging from 2,906 to 3,079 m, corresponding to a gradient in mean annual temperature of 2.4 °C. The amount of total soil N, of plant available N and soil C/N ratio decreased significantly with increasing altitude, whereas soil pH increased. Soil δ 15N decreased with increasing altitude from +2.2 to −2.1‰ and δ 15N of plant tissues (roots and leaves) decreased from −3.7 to −5.5‰. The large shift in soil δ 15N of 4.3‰ from the lowest to the highest site suggested substantial differences in N cycling in alpine and nival ecosystems in the alpine nival ecotone investigated. We concluded that N cycling at the alpine–nival ecotone is likely to be controlled by various factors: temperature, soil age and development, atmospheric N deposition and plant competition. Our results furthermore demonstrate that the alpine–nival ecotone may serve as a sensitive indicator of global change.

Keywords

Climate change High mountains Mineralization Nitrification Stable isotopes Temperature Radiocarbon 

Notes

Acknowledgements

We are highly grateful to Alexandra Kaniak and Angelika Kaufmann for help in the field and Tina Bell and Ansgar Kahmen for comments on earlier versions of the manuscript. We further acknowledge the University of Vienna for travel funds to A. Richter and W. Wanek.

References

  1. Adger N, Aggarwal P, Agrawala S, Alcamo J, Allali A, Anisimov O, Arnell N, Boko M, Canziani O, Carter T, Casassa G, Confalonieri U, Cruz RV and al. e (2007) IPCC 2007 climate change 2007: impacts, adaptation and vulnerability. Working Group II Contribution to the Intergovernmental Panel on Climate Change Fourth Assessment Report. Cambridge University Press. p 23Google Scholar
  2. Amato M, Ladd JN (1988) Assay for microbial biomass based on ninhydrin-reactive nitrogen in extracts of fumigated soil. Soil Biol Biochem 20:107–114CrossRefGoogle Scholar
  3. Amundson R, Austin AT, Schuur EAG, Yoo K, Matzek V, Kendall C, Uebersax A, Brenner D, Baisden WT (2003) Global patterns of the isotopic composition of soil and plant nitrogen. Global Biogeochemical Cycles 17, Art. no. 1031Google Scholar
  4. Atkin OK, Cummins WR (1994) The effect of root temperature on the induction of nitrate reductase activities and nitrogen uptake rates in Arctic plant species. Plant Soil 159:187–197CrossRefGoogle Scholar
  5. Barrett JE, Burke IC (2000) Potential nitrogen immobilization in grassland soils across a soil organic matter gradient. Soil Biol Biochem 32:1707–1716CrossRefGoogle Scholar
  6. Berendse F, Lammerts EJ, Olff H (1998) Soil organic matter accumulation and its implications for nitrogen mineralization and plant species composition during succession in coastal dune slacks. Plant Ecol 137:71–78CrossRefGoogle Scholar
  7. Bowman WD, Steltzer H, Rosenstiel TN, Cleveland CC, Meier CL (2004) Litter effects of two co-occurring alpine species on plant growth, microbial activity and immobilization of nitrogen. Oikos 104:336–344CrossRefGoogle Scholar
  8. Brenner DL, Amundson R, Baisden WT, Kendall C, Harden J (2001) Soil N and 15N variation with time in a California annual grassland ecosystem. Geochim Cosmochim Acta 65:4171–4186CrossRefGoogle Scholar
  9. Brooker R, Kikvidze Z, Pugnaire FI, Callaway RM, Choler P, Lortie CJ, Michalet R (2005) The importance of importance. Oikos 111:208–208CrossRefGoogle Scholar
  10. Callaway RM, Brooker RW, Choler P, Kikvidze Z, Lortie CJ, Michalet R, Paolini L, Pugnaire FI, Newingham B, Aschehoug ET, Armas C, Kikodze D, Cook BJ (2002) Positive interactions among alpine plants increase with stress. Nature 417:844–848PubMedCrossRefGoogle Scholar
  11. Chapin FS, Shaver GR, Giblin AE, Nadelhoffer KJ, Laundre JA (1995) Responses of Arctic tundra to experimental and observed changes in climate. Ecology 76:694–711CrossRefGoogle Scholar
  12. Choler P, Michalet R, Callaway RM (2001) Facilitation and competition on gradients in alpine plant communities. Ecology 82:3295–3308Google Scholar
  13. Cox JA, Whelan RJ (2000) Soil development of an artificial soil mix: nutrient dynamics, plant growth, and initial physical changes. Aust J Soil Res 38:465–477CrossRefGoogle Scholar
  14. de Kovel CGF, Van Mierlo A, Wilms YJO, Berendse F (2000) Carbon and nitrogen in soil and vegetation at sites differing in successional age. Plant Ecol 149:43–50CrossRefGoogle Scholar
  15. Dullinger S (1998) Vegetation des Schrankogel, Stubaier Alpen. University of Vienna, ViennaGoogle Scholar
  16. Evans RD, Bloom AJ, Sukrapanna SS, Ehleringer JR (1996) Nitrogen isotope composition of tomato (Lycopersicon esculentum Mill. cv. T-5) grown under ammonium or nitrate nutrition. Plant Cell Environ 19:1317–1323Google Scholar
  17. Garnett TP, Smethurst PJ (1999) Ammonium and nitrate uptake by Eucalyptus nitens: effects of pH and temperature. Plant Soil 214:133–140CrossRefGoogle Scholar
  18. Gottfried M, Pauli H, Grabherr G (1998) Prediction of vegetation patterns at the limits of plant life: a new view of the alpine–nival ecotone. Arct Alp Res 30:207–221CrossRefGoogle Scholar
  19. Gottfried M, Pauli H, Reiter K, Grabherr G (1999) A fine-scaled predictive model for changes in species distribution patterns of high mountain plants induced by climate warming. Divers Distrib 5:241–251CrossRefGoogle Scholar
  20. Gottfried M, Pauli H, Reiter K, Grabherr G (2002) Potential effects of climate change on apine and nival plants in the Alps. In: Körner C, Spehn EM (eds) Mountain biodiversity: a global assessment. Parthenon Publishing, London, New York, pp 213–223Google Scholar
  21. Grabherr G, Gottfried M, Pauli H (1994) Climate effects on mountain plants. Nature 369:448–448CrossRefGoogle Scholar
  22. Grogan P, Chapin FS (2000) Initial effects of experimental warming on above- and belowground components of net ecosystem CO2 exchange in arctic tundra. Oecologia 125:512–520CrossRefGoogle Scholar
  23. Handley LL, Raven JA (1992) The use of natural abundance of nitrogen isotopes in plant physiology and ecology. Plant Cell Environ 15:965–985CrossRefGoogle Scholar
  24. Hartley AE, Neill C, Melillo JM, Crabtree R, Bowles FP (1999) Plant performance and soil nitrogen mineralization in response to simulated climate change in subarctic dwarf shrub heath. Oikos 86:331–343CrossRefGoogle Scholar
  25. Haselwandter K, Read DJ (1980) Fungal associations of roots of dominant and sub-dominant plants in high-alpine vegetation systems with special reference to mycorrhiza. Oecologia 45:57–62CrossRefGoogle Scholar
  26. Hobbie SE (1996) Temperature and plant species control over litter decomposition in Alaskan tundra. Ecol Monogr 66:503–522CrossRefGoogle Scholar
  27. Högberg P (1997) Tansley review No 95 – 15N natural abundance in soil–plant systems. New Phytol 137:179–203CrossRefGoogle Scholar
  28. Jacot KA, Luscher A, Nosberger J, Hartwig UA (2000a) The relative contribution of symbiotic N2 fixation and other nitrogen sources to grassland ecosystems along an altitudinal gradient in the Alps. Plant Soil 225:201–211CrossRefGoogle Scholar
  29. Jacot KA, Luscher A, Nosberger J, Hartwig UA (2000b) Symbiotic N2 fixation of various legume species along an altitudinal gradient in the Swiss Alps. Soil Biol Biochem 32:1043–1052CrossRefGoogle Scholar
  30. Jonasson S, Havstrom M, Jensen M, Callaghan TV (1993) In-situ mineralization of nitrogen and phosphorus of Arctic soils after perturbations simulating climate change. Oecologia 95:179–186CrossRefGoogle Scholar
  31. Jonasson S, Michelsen A, Schmidt IK, Nielsen EV (1999) Responses in microbes and plants to changed temperature, nutrient, and light regimes in the arctic. Ecology 80:1828–1843CrossRefGoogle Scholar
  32. Jonasson S, Castro J, Michelsen A (2004) Litter, warming and plants affect respiration and allocation of soil microbial and plant C, N and P in arctic mesocosms. Soil Biol Biochem 36:1129–1139CrossRefGoogle Scholar
  33. Kandeler E, Gerber H (1988) Short-term assay of soil urease activity using colorimetric determination of ammonium. Biol Fertil Soils 6:68–72CrossRefGoogle Scholar
  34. Khan SA, Mulvaney RL, Brooks PD (1998) Diffusion methods for automated nitrogen-15 analysis using acidified disks. Soil Sci Soc Am J 62:406–412CrossRefGoogle Scholar
  35. Kikvidze Z, Pugnaire FI, Brooker RW, Choler P, Lortie CJ, Michalet R, Callaway RM (2005) Linking patterns and processes in alpine plant communities: a global study. Ecology 86:1395–1400CrossRefGoogle Scholar
  36. Klanderud K, Birks HJB (2003) Recent increases in species richness and shifts in altitudinal distributions of Norwegian mountain plants. Holocene 13:1–6CrossRefGoogle Scholar
  37. Kramer MG, Sollins P, Sletten RS, Swart PK (2003) N isotope fractionation and measures of organic matter alteration during decomposition. Ecology 84:2021–2025CrossRefGoogle Scholar
  38. Kullman L (2002) Rapid recent range-margin rise of tree and shrub species in the Swedish Scandes. J Ecol 90:68–77CrossRefGoogle Scholar
  39. McGonigle TP, Miller MH, Evans DG, Fairchild GL, Swan JA (1990) A new method which gives an objective-measure of colonization of roots by vesicular arbuscular mycorrhizal fungi. New Phytol 115:495–501CrossRefGoogle Scholar
  40. McKee KL, Feller IC, Popp M, Wanek W (2002) Mangrove isotopic (δ 15 N and δ 13 C) fractionation across a nitrogen vs. phosphorus limitation gradient. Ecology 83:1065–1075Google Scholar
  41. Metz B, Davidson OR, Bosch PR, Dave R, Meyer LA (2007) IPCC, 2007. Climate change 2007: mitigation. Contribution of Working group III to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge, UK, New York, p 35Google Scholar
  42. Michelsen A, Jonasson S, Sleep D, Havstrom M, Callaghan TV (1996) Shoot biomass, . δ 13. C, nitrogen and chlorophyll responses of two arctic dwarf shrubs to in situ shading, nutrient application and warming simulating climatic change. Oecologia 105:1–12CrossRefGoogle Scholar
  43. Michelsen A, Quarmby C, Sleep D, Jonasson S (1998) Vascular plant 15N natural abundance in heath and forest tundra ecosystems is closely correlated with presence and type of mycorrhizal fungi in roots. Oecologia 115:406–418CrossRefGoogle Scholar
  44. Moiseev PA, Shiyatov SG (2003) Vegetation dynamics at the tree line ecotone in the Ural Highlands, Russia. In: Nagy L, Grabherr G, Körner C, Thompson DBA (eds) Alpine biodiversity in Europe. Springer, Berlin Heidelberg New York, pp 423–435Google Scholar
  45. Morecroft MD, Marrs RH, Woodward FI (1992) Altitudinal and seasonal trends in soil nitrogen mineralization rate in the Scottish Highlands. J Ecol 80:49–56CrossRefGoogle Scholar
  46. Munroe JS, Bockheim JG (2001) Soil development in low-arctic tundra of the northern Brooks Range, Alaska, USA. Arct Antarct Alp Res 33:78–87CrossRefGoogle Scholar
  47. Nadelhoffer KJ, Fry B (1994) Nitrogen isotope studies in forest ecosystems. In: Lajtha K, Michener RH (eds) Stable isotopes in ecology and environmental science. Blackwell Scientific Publications, OxfordGoogle Scholar
  48. Nadelhoffer KJ, Giblin AE, Shaver GR, Laundre JA (1991) Effects of temperature and substrate quality on element mineralization in six Arctic soils. Ecology 72:242–253CrossRefGoogle Scholar
  49. Patzelt J (1976) Geologische Kartierung des Ötztalkristallins im Gebiet südlich der Amberger Hütte. Diplomkartierung Universität Achen. University of Aachen, AachenGoogle Scholar
  50. Pauli H, Gottfried M, Grabherr G (1999) Vascular plant distribution patterns at the low-temperature limits of plant life – the alpine–nival ecotone of Mount Schrankogel (Tyrol, Austria). Phytocoenologia 29:297–325Google Scholar
  51. Pauli H, Gottfried M, Grabherr G (2001) High summits of the Alps in a changing climate. The oldest observation series on high mountain plant diversity in Europe. In: Walther G-R, Burga CA, Edwards PJ (eds) “Fingerprints” of climate change: adapted behaviour and shifting species ranges. Kluwer Academic Publisher, New York, pp 139–149Google Scholar
  52. Pauli H, Gottfried M, Reier K, Klettner C, Grabherr G (2007) Signals of range expansions and contractions of vascular plants in the high Alps: observations (1994–2004) at the GLORIA*master site Schrankogel, Tyrol, Austria. Glob Chang Biol 13:147–156CrossRefGoogle Scholar
  53. Peterjohn WT, Melillo JM, Steudler PA, Newkirk KM, Bowles FP, Aber JD (1994) Responses of trace gas fluxes and N availability to experimentally elevated soil temperatures. Ecol Appl 4:617–625CrossRefGoogle Scholar
  54. Schinner F (1982) Soil microbial activities and litter decomposition related to altitude. Plant Soil 65:87–94CrossRefGoogle Scholar
  55. Schmidt IK, Jonasson S, Shaver GR, Michelsen A, Nordin A (2002) Mineralization and distribution of nutrients in plants and microbes in four arctic ecosystems: responses to warming. Plant Soil 242:93–106CrossRefGoogle Scholar
  56. Shaw MR, Harte J (2001) Response of nitrogen cycling to simulated climate change: differential responses along a subalpine ecotone. Glob Chang Biol 7:193–210CrossRefGoogle Scholar
  57. Steltzer H, Bowman WD (1998) Differential influence of plant species on soil nitrogen transformations within moist meadow Alpine tundra. Ecosystems 1:464–474CrossRefGoogle Scholar
  58. Stuiver M, Polach HA (1977) Reporting of 14C data – discussion. Radiocarbon 19:355–363Google Scholar
  59. Sveinbjornsson B, Davis J, Abadie W, Butler A (1995) Soil carbon and nitrogen mineralization at different elevations in the Chugach Mountains of South–Central Alaska, U.S.A. Arct Alp Res 27:29–37CrossRefGoogle Scholar
  60. Temperton VM, Mwangi PN, Scherer-Lorenzen M, Schmid B, Buchmann N (2007) Positive interactions between nitrogen-fixing legumes and four different neighbouring species in a biodiversity experiment. Oecologia 151:190–205PubMedCrossRefGoogle Scholar
  61. van Heerwaarden LM, Toet S, Aerts R (2003) Nitrogen and phosphorus resorption efficiency and proficiency in six sub-arctic bog species after 4 years of nitrogen fertilization. J Ecol 91:1060–1070CrossRefGoogle Scholar
  62. Vierheilig H, Schweiger P, Brundrett M (2005) An overview of methods for the detection and observation of arbuscular mycorrhizal fungi in roots. Physiol Plant 125:393–404Google Scholar
  63. Virtanen R (2003) The high mountain vegetation of the Scandes. In: Nagy L, Grabherr G, Körner C, Thompson DBA (eds) Alpine biodiversity in Europe. Springer, Berlin, pp 31–38Google Scholar
  64. Volder A, Bliss LC, Lambers H (2000) The influence of temperature and nitrogen source on growth and nitrogen uptake of two polar-desert species, Saxifraga caespitosa and Cerastium alpinum. Plant Soil 227:139–148CrossRefGoogle Scholar
  65. Walther GR, Beissner S, Burga CA (2005) Trends in the upward shift of alpine plants. J Veg Sci 16:541–548CrossRefGoogle Scholar
  66. Watson RT, Zinyowera MC, Moss RH, Dokken DJ (1997) IPCC special report. The regional impacts of climate change: an assessment of vulnerability. Cambridge University Press, Cambridge, U.K., p 527Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Edith Huber
    • 1
    • 2
  • Wolfgang Wanek
    • 1
  • Michael Gottfried
    • 3
  • Harald Pauli
    • 3
  • Peter Schweiger
    • 4
  • Stefan K. Arndt
    • 2
  • Karl Reiter
    • 3
  • Andreas Richter
    • 1
    • 2
  1. 1.Department of Chemical Ecology and Ecosystem ResearchUniversity of ViennaViennaAustria
  2. 2.School of Forest and Ecosystem ScienceThe University of MelbourneVictoriaAustralia
  3. 3.Department of Conservation Biology, Vegetation and Landscape EcologyUniversity of ViennaViennaAustria
  4. 4.Department of Forest and Soil ScienceUniversity of Natural Resources and Applied Life SciencesViennaAustria

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