Advertisement

Plant and Soil

, Volume 298, Issue 1–2, pp 99–111 | Cite as

Copper uptake and phytotoxicity as assessed in situ for durum wheat (Triticum turgidum durum L.) cultivated in Cu-contaminated, former vineyard soils

  • A. M. Michaud
  • M. N. Bravin
  • M. Galleguillos
  • P. HinsingerEmail author
Original Article

Abstract

This work assessed in situ, copper (Cu) uptake and phytotoxicity for durum wheat (Triticum turgidum durum L.) cropped in a range of Cu-contaminated, former vineyard soils (pH 4.2–7.8 and total Cu concentration 32–1,030 mg Cu kg−1) and identified the underlying soil chemical properties and related root-induced chemical changes in the rhizosphere. Copper concentrations in plants were significantly and positively correlated to soil Cu concentration (total and EDTA). In addition, Cu concentration in roots which was positively correlated to soil pH tended to be larger in calcareous soils than in non-calcareous soils. Symptoms of Cu phytotoxicity (interveinal chlorosis) were observed in some calcareous soils. Iron (Fe)–Cu antagonism was found in calcareous soils. Rhizosphere alkalisation in the most acidic soils was related to decreased CaCl2-extractable Cu. Conversely, water-extractable Cu increased in the rhizosphere of both non-calcareous and calcareous soils. This work suggests that plant Cu uptake and risks of Cu phytotoxicity in situ might be greater in calcareous soils due to interaction with Fe nutrition. Larger water extractability of Cu in the rhizosphere might relate to greater Cu uptake in plants exhibiting Cu phytotoxic symptoms.

Keywords

Copper Iron pH Phytotoxicity Rhizosphere Triticum turgidum durum L. 

Notes

Acknowledgements

We thank Jean-Pierre Barthès, Philippe Braun and Alain Alies for their technical support in this research and for identifying the soils to be sampled. The availability of farmers is gratefully acknowledged. We also thank Marc Benedetti, Laurence Denaix and André Schneider for their constructive comments. Financial support for this work was provided by the French Ministry of Ecology and Sustainable Development through its PNETOX programme, as well as an ECCO-ECODYN project.

References

  1. Afnor (1999) Recueil de Normes Françaises. Qualité des sols. Afnor, ParisGoogle Scholar
  2. Baize D (2000) Guide des analyses en pédologie, 2ème édition revue et augmentée. INRA, ParisGoogle Scholar
  3. Braun P (2006) Diagnostic des accidents du blé dur. ARVALIS-Institut du végétal, ParisGoogle Scholar
  4. Brun LA, Maillet J, Hinsinger P, Pépin M (2001) Evaluation of copper availability to plants in copper-contaminated vineyard soils. Environ Pollut 111:293–302PubMedCrossRefGoogle Scholar
  5. Carrillo-Gonzalez R, Simünek J, Sauvé S, Adriano D (2006) Mechanisms and pathways of trace element mobility in soils. Adv Agron 91:113–180Google Scholar
  6. Cattani I, Fragoulis G, Boccelli R, Capri E (2006) Copper bioavailability in the rhizosphere of maize (Zea mays L.) grown in two Italian soils. Chemosphere 64:1972–1979PubMedCrossRefGoogle Scholar
  7. Chaignon V, Hinsinger P (2003) A biotest for evaluating copper bioavailability to plants in a contaminated soil. J Environ Qual 32:824–833PubMedCrossRefGoogle Scholar
  8. Chaignon V, Bedin F, Hinsinger P (2002a) Copper bioavailability and rhizosphere pH changes as affected by nitrogen supply for tomato and oilseed rape cropped on an acidic and a calcareous soil. Plant Soil 243:219–228CrossRefGoogle Scholar
  9. Chaignon V, Di Malta D, Hinsinger P (2002b) Fe-deficiency increases Cu acquisition by wheat cropped in a Cu-contaminated vineyard soil. New Phytol 154:121–130CrossRefGoogle Scholar
  10. Chaignon V, Sanchez-Neira I, Herrmann P, Jaillard B, Hinsinger P (2003) Copper bioavailability and extractability as related to chemical properties of contaminated soils from a vine-growing area. Environ Pollut 123:229–238PubMedCrossRefGoogle Scholar
  11. Cornu JY, Staunton S, Hinsinger P (2007) Copper concentration in plants and in the rhizosphere as influenced by the iron status of tomato (Lycopersicon esculentum L.). Plant Soil 292:63–77CrossRefGoogle Scholar
  12. Coullery P (1997) Gestion des sols faiblement pollués par des métaux lourds. Rev Suisse Agric 29:299–305Google Scholar
  13. Courchesne F, Kruyts N, Legrand P (2006) Labile zinc concentration and free copper ion activity in the rhizosphere of forest soils. Environ Toxicol Chem 25:635–642PubMedCrossRefGoogle Scholar
  14. Degryse F, Smolders E, Parker DR (2006) Metal complexes increase uptake of Zn and Cu by plants: implications for uptake and deficiency studies in chelator-buffered solutions. Plant Soil 289:171–185CrossRefGoogle Scholar
  15. Harmsen J, Rulkens W, Eijsackers H (2005) Bioavailability, concept for understanding or tool for predicting? Land Contam Reclam 13:161–171Google Scholar
  16. Hinsinger P, Courchesne F (2007) Mobility and bioavailability of heavy metals and metalloids at soil–root interface. In: Violante A, Huang PM, Gadd GM (eds) Biophysico-chemical processes of metals and metalloids in soil environments. John Wiley & sons (in press)Google Scholar
  17. Hinsinger P, Gobran GR, Gregory PJ, Wenzel WW (2005) Rhizosphere geometry and heterogeneity arising from root-mediated physical and chemical processes. New Phytol 168:293–303PubMedCrossRefGoogle Scholar
  18. ISO (1999) Soil quality. Guidance on the ecotoxicological characterisation of soils and soil materials. Guidelines no. ISO TC 190/SC 7 ISO/DIS 15799. ISO, Geneva, SwitzerlandGoogle Scholar
  19. Kochian LV, Hoekenga OA, Pineros MA (2004) How do crop plants tolerate acid soils? – Mechanisms of aluminium tolerance and phosphorous efficiency. Annu Rev Plant Biol 55:459–493PubMedCrossRefGoogle Scholar
  20. Kopittke PM, Menzies NW (2006) Effect of Cu toxicity on growth of cowpea (Vigna unguiculata). Plant Soil 279:287–296CrossRefGoogle Scholar
  21. Lang HJ, Reed DW (1987) Comparison of HCl extraction versus total iron analysis for iron tissue analysis. J Plant Nutr 10:795–804CrossRefGoogle Scholar
  22. Lebourg A, Sterckeman T, Ciesielshi H, Proix N (1998) Trace metal speciation in three unbuffered salt solutions used to assess their bioavailability in soil. J Environ Qual 27:584–590CrossRefGoogle Scholar
  23. Lexmond TM, Van der Vorm PDJ (1981) The effect of pH on copper toxicity to hydroponically grown maize. Neth J Agric Sci 29:217–238Google Scholar
  24. Ma JF, Ryan PR, Delhaize E (2001) Aluminium tolerance in plants and the complexing role of organic acids. Trends Plant Sci 6:273–278PubMedCrossRefGoogle Scholar
  25. Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic, London, UK, 889 ppGoogle Scholar
  26. McBride MB (1981) Forms and distribution of copper in solid and solution phase of soil. In: Lorenagan JF, Robson AD, Graham RD (eds) Copper in soils and plants. Academic, Australia, pp 25–45Google Scholar
  27. McBride MB (2001) Cupric ion activity in peat soil as a toxicity indicator for maize. J Environ Qual 30:78–84PubMedCrossRefGoogle Scholar
  28. Mengel K, Kirkby EA (2001) Principles of plant nutrition, 5th edn. Kluwer, DordrechtGoogle Scholar
  29. Nolan AL, Zhang H, McLaughlin MJ (2005) Prediction of zinc, cadmium, lead, and copper availability to wheat in contaminated soils using chemical speciation, diffuse gradients in thin films, extraction, and isotopic dilution techniques. J Environ Qual 34:496–507PubMedCrossRefGoogle Scholar
  30. Pietrzak U, McPhail DC (2004) Copper accumulation, distribution and fractionation in vineyard soils of Victoria, Australia. Geoderma 122:151–166CrossRefGoogle Scholar
  31. Reichman SM, Parker DR (2005) Metal complexation by phytosiderophores in the rhizosphere. In: Huang PM, Bobran GR (eds) Biogeochemistry of trace elements in the rhizosphere. Elsevier, Toronto, pp 129−156Google Scholar
  32. Reichman SM, Parker DR (2007) Probing the effects of light and temperature on diurnal rhythms of phytosiderophore release in wheat. New Phytol 174:101–108PubMedCrossRefGoogle Scholar
  33. Rengel Z (1996) Tansley review no 89 – uptake of aluminium by plant cells. New Phytol 134:389–406CrossRefGoogle Scholar
  34. Rieuwerts JS, Thornton I, Farago ME, Ashmore MR (1998) Factors influencing metal bioavailability in soils, preliminary investigations for the development of a critical loads approach for metals. Chem Speciat Bioavailab 10:61–75Google Scholar
  35. Römkens PFAM, Bouwman LA, Boon GT (1999) Effect of plant growth on copper solubility and speciation in soil solution samples. Environ Pollut 106:315–321PubMedCrossRefGoogle Scholar
  36. Sattelmacher B (2001) The apoplast and its significance for plant mineral nutrition. Tansley review, 22. New Phytol 149:167–192CrossRefGoogle Scholar
  37. Sauvé S, Cook N, Hendershot WH, McBride MB (1996) Linking plant tissue concentrations and soil copper pools in urban contaminated soils. Environ Pollut 94:153–157PubMedCrossRefGoogle Scholar
  38. Sauvé S, McBride MB, Norvell WA, Hendershot WH (1997) Copper solubility and speciation of in situ contaminated soils: effects of copper level, pH and organic matter. Water Air Soil Pollut 100:133–149CrossRefGoogle Scholar
  39. Thakali S, Allen HE, di Toro D, Ponizovsky AA, Rooney CP, Zhao FJ, McGrath SP (2006a) A terrestrial biotic ligand model. 1. Development and application to Cu and Ni toxicities to barley root elongation in soils. Environ Sci Technol 40:7085–7093CrossRefGoogle Scholar
  40. Thakali S, Allan HE, di Toro DM, Ponizovsky AA, Rooney CP, Zhao FJ, McGrath SP, Criel P, van Eeckhout H, Janssen CR, Oorts K, Smolders E (2006b) Terrestrial biotic ligand model. 2. Application to Ni and Cu toxicities to plants, invertebrates, and microbes in soil. Environ Sci Technol 40:7094–7100CrossRefGoogle Scholar
  41. Treeby M, Marschner H, Römheld V (1989) Mobilization of iron and other micronutrient cations from a calcareous soil by plant-borne, microbial, and synthetic metal chelators. Plant Soil 114:217–226CrossRefGoogle Scholar
  42. Vulkan R, Zhao FJ, Barbosa-Jefferson V, Preston S, Paton GI, McGrath SP (2000) Copper speciation and impacts on bacterial biosensors in the pore water of copper-contaminated soils. Environ Sci Technol 34:5115–5121CrossRefGoogle Scholar
  43. Wheeler DM, Power IL (1995) Comparison of plant uptake and plant toxicity of various ions in wheat. Plant Soil 172:167–173CrossRefGoogle Scholar
  44. Zhao FJ, Rooney CP, Zhang H, McGrath SP (2006) Comparison of soil solution speciation and diffusive gradients in thin-films measurement as an indicator of copper bioavailability to plants. Environ Toxicol Chem 25:733–742PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • A. M. Michaud
    • 1
  • M. N. Bravin
    • 1
  • M. Galleguillos
    • 1
  • P. Hinsinger
    • 1
    Email author
  1. 1.INRA–SupAgroUMR 1222 Biogéochimie du Sol et de la RhizosphèreMontpellier cedex 1France

Personalised recommendations