Copper uptake and phytotoxicity as assessed in situ for durum wheat (Triticum turgidum durum L.) cultivated in Cu-contaminated, former vineyard soils
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This work assessed in situ, copper (Cu) uptake and phytotoxicity for durum wheat (Triticum turgidum durum L.) cropped in a range of Cu-contaminated, former vineyard soils (pH 4.2–7.8 and total Cu concentration 32–1,030 mg Cu kg−1) and identified the underlying soil chemical properties and related root-induced chemical changes in the rhizosphere. Copper concentrations in plants were significantly and positively correlated to soil Cu concentration (total and EDTA). In addition, Cu concentration in roots which was positively correlated to soil pH tended to be larger in calcareous soils than in non-calcareous soils. Symptoms of Cu phytotoxicity (interveinal chlorosis) were observed in some calcareous soils. Iron (Fe)–Cu antagonism was found in calcareous soils. Rhizosphere alkalisation in the most acidic soils was related to decreased CaCl2-extractable Cu. Conversely, water-extractable Cu increased in the rhizosphere of both non-calcareous and calcareous soils. This work suggests that plant Cu uptake and risks of Cu phytotoxicity in situ might be greater in calcareous soils due to interaction with Fe nutrition. Larger water extractability of Cu in the rhizosphere might relate to greater Cu uptake in plants exhibiting Cu phytotoxic symptoms.
KeywordsCopper Iron pH Phytotoxicity Rhizosphere Triticum turgidum durum L.
We thank Jean-Pierre Barthès, Philippe Braun and Alain Alies for their technical support in this research and for identifying the soils to be sampled. The availability of farmers is gratefully acknowledged. We also thank Marc Benedetti, Laurence Denaix and André Schneider for their constructive comments. Financial support for this work was provided by the French Ministry of Ecology and Sustainable Development through its PNETOX programme, as well as an ECCO-ECODYN project.
- Afnor (1999) Recueil de Normes Françaises. Qualité des sols. Afnor, ParisGoogle Scholar
- Baize D (2000) Guide des analyses en pédologie, 2ème édition revue et augmentée. INRA, ParisGoogle Scholar
- Braun P (2006) Diagnostic des accidents du blé dur. ARVALIS-Institut du végétal, ParisGoogle Scholar
- Carrillo-Gonzalez R, Simünek J, Sauvé S, Adriano D (2006) Mechanisms and pathways of trace element mobility in soils. Adv Agron 91:113–180Google Scholar
- Coullery P (1997) Gestion des sols faiblement pollués par des métaux lourds. Rev Suisse Agric 29:299–305Google Scholar
- Harmsen J, Rulkens W, Eijsackers H (2005) Bioavailability, concept for understanding or tool for predicting? Land Contam Reclam 13:161–171Google Scholar
- Hinsinger P, Courchesne F (2007) Mobility and bioavailability of heavy metals and metalloids at soil–root interface. In: Violante A, Huang PM, Gadd GM (eds) Biophysico-chemical processes of metals and metalloids in soil environments. John Wiley & sons (in press)Google Scholar
- ISO (1999) Soil quality. Guidance on the ecotoxicological characterisation of soils and soil materials. Guidelines no. ISO TC 190/SC 7 ISO/DIS 15799. ISO, Geneva, SwitzerlandGoogle Scholar
- Lexmond TM, Van der Vorm PDJ (1981) The effect of pH on copper toxicity to hydroponically grown maize. Neth J Agric Sci 29:217–238Google Scholar
- Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic, London, UK, 889 ppGoogle Scholar
- McBride MB (1981) Forms and distribution of copper in solid and solution phase of soil. In: Lorenagan JF, Robson AD, Graham RD (eds) Copper in soils and plants. Academic, Australia, pp 25–45Google Scholar
- Mengel K, Kirkby EA (2001) Principles of plant nutrition, 5th edn. Kluwer, DordrechtGoogle Scholar
- Reichman SM, Parker DR (2005) Metal complexation by phytosiderophores in the rhizosphere. In: Huang PM, Bobran GR (eds) Biogeochemistry of trace elements in the rhizosphere. Elsevier, Toronto, pp 129−156Google Scholar
- Rieuwerts JS, Thornton I, Farago ME, Ashmore MR (1998) Factors influencing metal bioavailability in soils, preliminary investigations for the development of a critical loads approach for metals. Chem Speciat Bioavailab 10:61–75Google Scholar
- Thakali S, Allan HE, di Toro DM, Ponizovsky AA, Rooney CP, Zhao FJ, McGrath SP, Criel P, van Eeckhout H, Janssen CR, Oorts K, Smolders E (2006b) Terrestrial biotic ligand model. 2. Application to Ni and Cu toxicities to plants, invertebrates, and microbes in soil. Environ Sci Technol 40:7094–7100CrossRefGoogle Scholar