Plant and Soil

, Volume 290, Issue 1–2, pp 259–268 | Cite as

A method for assessing arbuscular mycorrhizal fungi group distribution in tree roots by intergenic transcribed sequence variation

  • Mervyn Shepherd
  • Linh Nguyen
  • Megan E. Jones
  • J. Doland Nichols
  • F. Lynn Carpenter
Original Paper

Abstract

We identified five taxonomic groups of arbuscular mycorrhizal fungi (AMF) inside roots of young trees of six species of legumes and six species of non-legumes from a field site in southern Costa Rica using an AMF group-specific PCR assay of the intergenic transcribed sequence and 18S rRNA gene fragment. Assay specificity was verified by cloning and sequencing representatives from four of the five AMF groups. We found no difference in overall AMF diversity levels between legumes and non-legumes or between plant species. Some groups of AMF may associate more frequently with legumes than others, as Glomus Group A (Glomus mosseae/intradices group) representatives were detected more frequently in legumes than non-legumes relative to Glomus Group B (Glomus etunicatum/claroideum) representatives.

Keywords

Acaulosporaceae Gigasporaceae Glomales AMF diversity 

Notes

Acknowledgments

The authors thank Eduer Sandi Tapia, Rolando Mendez Rodriguez, and Leonel Figueroa Vargas for their conscientious assistance in the field work. We thank F. Eliott for technical assistance in the laboratory. Financial support was provided by University of California Research Expeditions Program and Rainforest Agrarian International Network.

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Mervyn Shepherd
    • 1
  • Linh Nguyen
    • 1
  • Megan E. Jones
    • 1
  • J. Doland Nichols
    • 2
  • F. Lynn Carpenter
    • 3
  1. 1.Centre for Plant Conservation GeneticsSouthern Cross UniversityLismoreAustralia
  2. 2.Sustainable Forestry Program, School of Environmental Science and ManagementSouthern Cross UniversityLismoreAustralia
  3. 3.Department of Ecology and Evolutionary BiologyUniversity of CaliforniaIrvineUSA

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