Plant and Soil

, Volume 289, Issue 1–2, pp 301–308 | Cite as

Availability of iodide and iodate to spinach (Spinacia oleracea L.) in relation to total iodine in soil solution

  • J. L. Dai
  • Y. G. ZhuEmail author
  • Y. Z. Huang
  • M. Zhang
  • J. L. Song
Original Paper


A greenhouse pot experiment was carried out to investigate the availability of iodide and iodate to soil-grown spinach (Spinacia oleracea L.) in relation to total iodine concentration in soil solution. Four iodine concentrations (0, 0.5, 1, 2 mg kg−1) for iodide (I) and iodate (IO 3 ) were used. Results showed that the biomass productions of spinach were not significantly affected by the addition of iodate and iodide to the soil, and that iodine concentrations in spinach plants on the basis of fresh weights increased with increasing addition of iodine. Iodine concentrations in tissues were much greater for plants grown with iodate than with iodide. In contrast to the iodide treatments, in iodate treatment leaves accounted for a larger fraction of the total plant iodine. The soil-to-leaf transfer factors (TFleaf) for plants grown with iodate were about tenfold higher than those grown with iodide. Iodine concentrations in soil solution increased with increasing iodine additions to the soil irrespective of iodine species. However, total iodine in soil solution was generally higher for iodate treatments than iodide both in pots with and without spinach. According to these results, iodate can be considered as potential iodine fertilizer to increase iodine content in vegetables.


Biofortification Iodide Iodate Spinach Soil solution 



This project was financially supported by the Chinese Academy of Sciences (KZCX1-SW−19 and Hundred Talent Program). We thank Professor Andrew Smith for critical reading of the manuscript and polishing the English.


  1. Borst GW, Pauwels FH (1961) Iodine as a micronutrient for plants. Plant Soil 14:377–392CrossRefGoogle Scholar
  2. Dai JL, Zhu YG, Zhang M, Huang YZ (2004a) Selecting iodine-enriched vegetables and residual effectiveness of iodate application to soil. Biol Trace Elem Res 101:265–276CrossRefGoogle Scholar
  3. Dai JL, Zhu YG, Zhang M (2004b) Determination of iodide in xylem sap of spinach by ion chromatography with pulsed amperometry detector. Environ Chem 23:351–352 (in Chinese)Google Scholar
  4. Dai JL, Zhang M, Zhu YG (2004c) Adsorption and desorption of iodine by various Chinese soils: I. Iodate. Environ Int 30:525–530CrossRefGoogle Scholar
  5. Dimmer CH, Simmonds PG, Nickless G, Bassford MR (2001) Biogenic fluxes of halomethanes from Irish peatland ecosystems. Atmos Environ 35:321–330CrossRefGoogle Scholar
  6. Fang R, She XL, Zhong ZH (1994) Ion chromatography (IC) with amperometric detection for the measurement of trace amount of iodine in serum, urine and hair. Chin J Chromatogr 12:150–151 (in Chinese)Google Scholar
  7. Fuge R (1996) Geochemistry of iodine in relation to iodine deficiency disease. In: Appleton JD, Fuge R, Mccall GJH (eds) Environmental geochemistry and health. Geological Society Special Publication, London, 113, pp 201–211Google Scholar
  8. Gong ZT (1999) The system classify of Chinese soil. Science publishing company published, Beijing (in Chinese)Google Scholar
  9. Hou XL, Chai CF, Qian QF et al (1997) The study of iodine in Chinese total diets. Sci Total Environ 193:161–167PubMedCrossRefGoogle Scholar
  10. Johnson CC, Strutt MH, Hmeurras M, Mounir M (2002) Iodine in the environment of the High Atlas Mountain area of Morocco. British Geological Survey, Keyworth, Nottingham, UK, Commisioned Report, CR/02/196Google Scholar
  11. Jopke P, Bahadir M, Fleckenstein J, Schnug E (1996) Iodine determination in plant materials. Commun Soil Sci Plant Anal 27:741–751CrossRefGoogle Scholar
  12. Keppler F, Borchers R, Elsner P, Fahimi I, Pracht J, SchÖler HF (2003) Formation of volatile iodinated alkanes in soil: results from laboratory studies. Chemosphere 52:477–483PubMedCrossRefGoogle Scholar
  13. Keppler F, Eiden R, Niedan V, Pracht J, SchÖler HF (2000) Halocarbons produced by natural oxidation processes during degradation of organic matter. Nature 403:298–301PubMedCrossRefGoogle Scholar
  14. Lu RK (2000) The analysis method of soil agricultural chemistry, the Chinese Society of Soil Science edited. China agricultural science and technology publishing company published. (in Chinese)Google Scholar
  15. Mackowiak CL, Grossl PR (1999) Iodate and iodide effects on iodine uptake and partitioning in rice (Oryza sativa L.) grown in solution culture. Plant Soil 212:135–143PubMedCrossRefGoogle Scholar
  16. Muramatsu Y, Yoshida S, Bannai T (1995) Trace experiments on the behavior of radioiodine in the soil–plant–atmosphere system. J Radioanal Nucl Chem-Articles 194:303–310CrossRefGoogle Scholar
  17. Mynet A, Wain RL (1973) Herbicidal action of iodide: effect on chlorophyll content and photosynthesis in dwarf bean Phaseolus vulgaris. Weed Res 13:101–109CrossRefGoogle Scholar
  18. Redeker KR, Wang NY, Low JC, McMillan A, Tyler SC Cicerone (2000) Emissions of methyl halides and methane from rice paddies. Science 290:966–969PubMedCrossRefGoogle Scholar
  19. Shinonaga T, Casta J, Muck K, Gerzabek MH (2000) Determination of iodine in cereal grains and standard reference materials by neutron activation analysis. J Environ Anal Chem 78:175–84Google Scholar
  20. Whitehead DC (1973) Uptake and distribution of iodine in grass and clover plants growth in solution culture. J Sci Food Agric 24:43–50PubMedCrossRefGoogle Scholar
  21. Xinjiang Disease Control Centre (XJDCC) (2000) Survey report of iodine deficiency disorders in Xinjiang Province, China in 1999. Endem Dis Bull 15:59–63Google Scholar
  22. Yuita K (1982) Iodine, bromine, and chlorine contents in soils and plants of Japan. I. Iodine, bromine and chlorine contents in soils and plants of the basin of the Miomote rive. Soil Sci Plant Nutr 28:315–336Google Scholar
  23. Yuita K (1992) Dynamics of iodine, bromine, and chlorine soil: II. Chemical forms of iodine in soil solutions. Soil Sci Plant Nutr 38:281–287Google Scholar
  24. Zhang L, Chen Z, Wang J, Bao J (2000) Iodine loss from iodinised salt during processing, sale and consumption. Zhejiang Prev Med 12:32–34Google Scholar
  25. Zhu YG, Huang YZ, Hu Y, Liu YX (2003) Iodine uptake by spinach (Spinacia oleracea L.) plants grown in solution culture: effects of iodine species and solution concentrations. Environ Int 29:33–37PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • J. L. Dai
    • 1
    • 2
    • 3
  • Y. G. Zhu
    • 1
    Email author
  • Y. Z. Huang
    • 1
  • M. Zhang
    • 2
  • J. L. Song
    • 2
  1. 1.Research Center for Eco-environmental Sciences, Chinese Academy of SciencesBeijingChina
  2. 2.College of Environment and ResourceShandong Agricultural UniversityTai’anChina
  3. 3.Environment Research InstituteShandong UniversityJi’nanChina

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