Plant and Soil

, Volume 287, Issue 1–2, pp 51–58

Effect of Tilemsi phosphate rock-solubilizing microorganisms on phosphorus uptake and yield of field-grown wheat (Triticum aestivum L.) in Mali

Article

Abstract

With the broad aim of biologically improving P uptake by wheat fertilized with Tilemsi phosphate rock (TPR), we investigated the effect of inoculation with TPR-solubilizing microorganisms isolated from Malian soils and with a commercial isolate of the arbuscular mycorrhizal (AM) fungus Glomus intraradices (Gi). AM root length colonization, and growth yield and P concentration of the cultivar Tetra of wheat were measured under field conditions in Mali. Experimental plots were established in Koygour (Diré) during the 2001–2002 cropping season. Inoculation treatments included two fungal isolates, Aspergillus awamori (C1) and Penicillium chrysogenum (C13), and an isolate of Pseudomonas sp. (BR2), used alone or in fungus-bacterium combinations in the presence or absence of the AM fungus Gi. In fertilized treatments, 0 or 30 kg P ha−1 was applied as TPR or diammonium phosphate (DAP). In 45-day-old wheat plants, the highest root length AM colonization (62%) was observed with TPR fertilized wheat inoculated with Gi and BR2. Our results suggest that BR2 is a mycorrhizal-helper bacteria and a good plant growth-promoting rhizobacteria. In fact, inoculation of wheat Tetra fertilized with TPR with a combination of Gi, BR2 and C1 produced the best grain yield with the highest P concentration. This work shows that by inoculating seeds with TPR-solubilizing microorganisms and AM fungi under field conditions in Mali it is possible to obtain wheat grain yields comparable to those produced by using the expensive DAP fertilizer.

Keywords

Aspergillus awamori Glomus intraradices Plant growth promotion Penicillium chrysogenum Pseudomonas sp. Triticum aestivum 

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References

  1. Barea J M, Azcón R, Azcón-Aguilar C 2002 Mycorhizosphere interactions to improve plant fitness and soil quality Antonie van Leeuwenhoek 81: 343–351PubMedCrossRefGoogle Scholar
  2. Barea J M, Andrade G, Bianciotto V, Dowling D, Lohrke S, Bonfante P, O’Gara F, Azcón-Aguilar C. 1998 Impact on arbuscular mycorrhiza formation of Pseudomonas strains used as inoculants for biocontrol of soil-borne fungal plant pathogens Appl. Environ. Microbiol. 64: 2304–2307PubMedGoogle Scholar
  3. Barea J M, Escudero J L, Azcón-Aguilar C 1980 Effects of introduced and indigenous VA mycorrhizal fungi on nodulation, growth and nutrition of Medicago sativa in phosphate-fixing soils as affected by P-fertilizers Plant and Soil 54: 283–296CrossRefGoogle Scholar
  4. Bationo A, Ayuk E, Ballo D, Koné M 1997 Agronomic and economic evaluation of Tilemsi phosphate rock in different agroecological zones of Mali Nutrient Cycling Agrosyst. 48: 179–189CrossRefGoogle Scholar
  5. Bolland M D A, Gilkes R J 1997 The agronomic effectiveness of reactive phosphate rocks. 2. Effect of phosphate rock reactivity Aust. J. Exp. Agric. 37: 937–946CrossRefGoogle Scholar
  6. Chabot R, Antoun H, Cescas M P 1993 Stimulation de la croissance du maïs et de la laitue romaine par des microorganismes dissolvant le phosphore inorganique Can. J. Microbiol. 39: 941–947CrossRefGoogle Scholar
  7. Chabot R, Antoun H, Cescas M P 1996 Growth promotion of maize and lettuce by phosphate-solubilizing Rhizobium leguminosarum biovar phaseoli Plant and Soil 184: 311–321CrossRefGoogle Scholar
  8. Diop T, Gueye M, Dreyfus B, Plenchette C, Strullu D G 1994 Indigenous arbuscular mycorrhizal fungi associated with Acacia albida Del. in different areas of Senegal Appl. Environ. Microbiol. 60: 3433–3436PubMedGoogle Scholar
  9. Gahoonia T S, Care D, Nielsen N E 1997 Root hairs and phosphorus acquisition of wheat and barley cultivars Plant and Soil 191:181–188CrossRefGoogle Scholar
  10. Gaines P T, Mitchell A G 1979 Chemical methods for Soil and Plant Analysis University of Georgia, Coastal Plain Station, Tifton, USA, 105pGoogle Scholar
  11. Germida J J, Walley F L 1996 Plant growth-promoting rhizobacteria alter rooting patterns and arbuscular mycorrhizal fungi colonization of field-grown spring wheat Biol. Fertil. Soils 23: 113–120Google Scholar
  12. Goldstein A H 1986 Bacterial solubilization of mineral phosphates: Historical perspective and future prospects Am. J. Altern. Agric. 1: 51–57Google Scholar
  13. Graham J H, Abbott L K 2000 Wheat responses to aggressive and non-aggressive arbuscular mycorrhizal fungi Plant and Soil 220: 207–218CrossRefGoogle Scholar
  14. Gulden R H, Vessey J K 2000 Penicillium bilaii inoculation increases root-hair production in field pea Can. J. Plant Sci. 80: 801–804Google Scholar
  15. Large E C 1954 Growth stages in cereals: Illustration of the Feekes scale Plant Pathol. 3: 128–129Google Scholar
  16. McGee P 1989 Variations in propagules number of vesicular–arbuscular mycorrhizal fungi in a semi-arid soil Mycol. Res. 92: 28–33CrossRefGoogle Scholar
  17. McKeague J A Ed. 1978 Manual of Soil Sampling and Methods of Analysis. Canadian Soil Survey Committee, Canadian Society of Soil Science, Ottawa, 223 ppGoogle Scholar
  18. Mehlich A 1984 Mehlich 3 soil test extractant: A modification of Mehlich 2 extractant Commun. Soil Sci. Plant Anal. 15: 1409–1416CrossRefGoogle Scholar
  19. Nautiyal C S 1999 An efficient microbiological medium for screening phosphate solubilizing microorganisms FEMS Microbiol. Lett. 170: 265–270PubMedCrossRefGoogle Scholar
  20. Omar S A 1998 The role of rock-phosphate-solubilizing fungi and vesicular–arbuscular mycorrhiza (VAM) in growth of wheat plants fertilized with rock phosphate World J. Microbiol. Biotech. 14: 211–218CrossRefGoogle Scholar
  21. Reyes I, Bernier L, Simard R R, Antoun H 1999 Effect of nitrogen source on the solubilization of different inorganic phosphates by an isolate of Penicillium rugulosum and two UV-induced mutants FEMS Microbiol. Ecol. 28: 281–290CrossRefGoogle Scholar
  22. Reyes I, Baziramakenga R, Bernier L, Antoun H 2001 Solubilization of phosphate rocks and minerals by a wild-type strain and two UV-induced mutants of Penicillium rugulosum Soil Biol. Biochem. 33: 1741–1747CrossRefGoogle Scholar
  23. Reyes I, Bernier L, Antoun H 2002 Rock phosphate solubilization and colonization of maize rhizosphere by wild and genetically modified strains of Penicillium rugulosum Microb. Ecol. 44: 39–48PubMedCrossRefGoogle Scholar
  24. Richardson A E 2001 Prospects for using soil microorganisms to improve the acquisition of phosphorus by plants Aust. J. Plant Physiol. 28: 897–906Google Scholar
  25. Rodriguez H, Fraga R 1999 Phosphate solubilizing bacteria and their role in plant growth promotion Biotech. Adv. 17: 319–339CrossRefGoogle Scholar
  26. SAS, Institute Inc. 1990 SAS Procedure Guide Version 6 3 edition, SAS Institute Inc, Cary, NC 705 pp. Google Scholar
  27. Singh S, Kapoor K K 1999 Inoculation with phosphate-solubilizing microorganisms and a vesicular–arbuscular mycorrhizal fungus improves dry matter yield and nutrient uptake by wheat grown in a sandy soil Biol. Fertil. Soils 28: 139–144CrossRefGoogle Scholar
  28. Sylvia D M, Chelleni D O 2001 Interactions among root-inhabiting fungi and their implications for biological control of root pathogens Adv. Agron. 73: 1–33Google Scholar
  29. Tandon H L S, Cescas M P, Tyner E H 1968 An acid-free vanadate–molybdate reagent for the determination of total phosphorus in soils Soil Sci. Soc. Am. Proc. 32: 48–51CrossRefGoogle Scholar
  30. Vierheilig H, Goughlan A P, Wyss U, Piché Y 1998 Ink and vinegar, a simple technique for arbuscular-mycorrhizal fungi Appl. Environ. Microbiol. 64: 5004–5007PubMedGoogle Scholar
  31. Villegas J, Fortin J A 2001 Phosphorus solubilization and pH changes as a result of the interactions between soil bacteria and arbuscular mycorrhizal fungi on a medium containing NH4+ as nitrogen source Can. J. Bot. 79: 865–870CrossRefGoogle Scholar
  32. Whitelaw M A 2000 Growth promotion of plants inoculated with phosphate-solubilizing fungi Adv. Agron. 69: 99–151Google Scholar
  33. Zhu Y-G, Smith S E 2001 Seed phosphorus (P) content affects growth, and P uptake of wheat plants and their association with arbuscular mycorrhizal (AM) fungi Plant and Soil 231: 105–112CrossRefGoogle Scholar
  34. Zhu Y-G, Smith S E, Barritt A R, Smith F A 2001 Phosphorus (P) efficiencies and mycorrhizal responsiveness of old and modern wheat cultivars Plant and Soil 237: 249–255CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.Faculté des Sciences et TechniquesUniversité du MaliBamakoMali
  2. 2.Département des Sols et de Génie Agroalimentaire, Faculté des Sciences de l’Agriculture et de l’AlimentationUniversité LavalQuébecCanada

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