Plant and Soil

, Volume 267, Issue 1–2, pp 13–22 | Cite as

Impact of tillage on the incidence ofFusarium spp. in soil

Article

Abstract

The influence of several long-term conventional and conservation tillage treatments on the incidence and the diversity ofFusarium spp. in soil was studied. Soil samples were randomly collected from naturally contaminated field trials and Fusarium species were isolated by using the dilution plate method. The identification of the species was done by direct microscopic observation onFusarium-specific media. The isolation frequency ofFusarium species and the total number of colony forming units was affected by the sampling year and the cultivated crop and showed significant differences between the tillage treatments. Moldboard plough-treatments resulted in a lower diversity of Fusarium species than the chisel plough and rotary tiller treatments. Besides the tillage system the tillage depth also appeared to affect theFusarium populations. The deeper the tillage the lower was the number of isolatedFusarium spp. TwentyFusarium species were identified over both years of investigation. In conservation tillage plots a higher diversity ofFusarium species was found than in the moldboard plough-based tillage plots. A correlation betweenFusarium species producing the mycotoxin deoxynivalenol (DON) isolated from soil and the DON-content of grain could not be observed. However, these investigations indicate that conservation soil tillage results in conditions which increase the incidence ofFusarium species in soil.

Key words

Fusarium spp. soilborne fungi tillage depth tillage treatment 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adler A, Lew H und Edinger W 1990 Vorkommen und Toxigenität vonFusarien auf Getreide und Mais aus Österreich. Bodenkultur 41, 145–152.Google Scholar
  2. Arnold-Reimer K 1994 Einfluss konservierender Bodenbearbeitung auf Pflanzenkrankheiten und Unkräuter im Getreide und Konsequenzen für einen gezielten Pflanzenschutz. Dissertation der Georg-August-Universität Göttingen.Google Scholar
  3. Bahle F und Leist N 1997 Einfluss konventioneller, integrierter und ökologischer Wirtschaftsweise sowie einzelner anbautechnischer Maßnahmen auf den Befall von Winterweizen mit samenbürtigen Pilzen. Ges. Pfl. 49, 220–225.Google Scholar
  4. Bailey K L and Duczek L J 1996 Managing cereal diseases under conservation tillage systems. Can. J. Plant Pathol. 18, 159–167.CrossRefGoogle Scholar
  5. Beck R und Lepschy J 2000 Ergebnisse aus demFusarium-Monitoring 1989–1999 - Einfluss der produktionstechnischen Faktoren Fruchtfolge und Bodenbearbeitung. In Risiken durch den ÄhrenparasitenFusarium graminearum - Ergebnisse eines LBP-Forschungsverbundes. Ed. Bayrische Landesanstalt für Bodenkultur und Pflanzenbau. pp. 39–47. Druckhaus Kastner, Wolznach.Google Scholar
  6. Bockus W W and Shroyer J P 1998 The impact of reduced tillage on soilborne plant pathogens. Ann. Rev. Phytopathol. 36, 485–500.Google Scholar
  7. Burgess L W 1981 General ecology of the Fusaria. InFusarium. Eds. P E Nelson, T A Toussoun and R J Cook. pp. 227–235. The Pennsylvania State University Press, University State and London.Google Scholar
  8. Burgess L W, Backhouse D, Summerell B A and Swan L J 2001 Crown rot of wheat. InFusarium. Eds. B A Summerell, J F Leslie, D Backhouse, W L Bryden and L W Burgess. pp. 271–294. APS Press, St. Paul, Minnesota.Google Scholar
  9. Codex Alimentarium Commission 2002 Discussion paper on deoxynivalenol. Joint FAO/WHO Food Standards Program. Codex Committee on Food Additives and Contaminants. ftp://ftp.fao.org/codex/ccfac35/fa03_35e.pdfGoogle Scholar
  10. Conway K E 1996 An overview of the influence of sustainable agricultural systems on plant diseases. Crop Prot. 15, 223–228.Google Scholar
  11. Cook R J 1973 Influence of low plant and soil water potentials on diseases caused by soilborne fungi. Phytopathology 73, 451–458.Google Scholar
  12. Cook R J 1981a Water relations in the biology ofFusarium. In Fusarium: Diseases, biology, and taxonomy. Eds. P E Nelson, T A Toussoun and R J Cook. pp. 236–244. The Pennsylvania State University Press, University State and London.Google Scholar
  13. Cook R J 1981bFusarium diseases of wheat and other small grains in North America. InFusarium. Eds. P E Nelson, T A Toussoun and R J Cook. pp. 39–52. The Pennsylvania State University Press, University State and London.Google Scholar
  14. Damm U 1998a Besiedlung von Weizenpflanzen mitFusarium- undRhizoctonia-Arten und Auftreten von Halmbasiserkrankungen bei unterschiedlicher Bewirtschaftungsintensität. Mitt. a. d. Biol. Bundesanst. 357, 86–88.Google Scholar
  15. Damm U 1998b Bodenmykoflora in zwei unterschiedlich bewirtschafteten Weizenbeständen mit besonderer Berücksichtigung derFusarium-Arten. Mitt. a. d. Biol. Bundesanst. 357, 90–91.Google Scholar
  16. Dill-Macky R and Jones R K 2000 The effect of previous crop residues and tillage onFusarium head blight of wheat. Plant Dis. 84, 71–76.Google Scholar
  17. Domsch H K und Gams W 1970 Pilze aus Agrarböden. Gustav Fischer Verlag Stuttgart. 222 pp.Google Scholar
  18. Flett B C, McLaren N W and Wehner F C 1998 Incidence of ear rot pathogens under alternating corn tillage practices. Plant Dis. 82, 781–784.Google Scholar
  19. Gang G, Miedaner T, Schuhmacher U, Schollenberger M and Geiger H H 1998 Deoxynivalenol and nivalenol production byFusarium culmorum isolates differing in aggressiveness toward winter rye. Phytopathology 88, 879–884.PubMedGoogle Scholar
  20. Garrett S D 1970 Pathogenic root-infecting fungi. Cambridge at the University Press, London 294 pp.Google Scholar
  21. Gerlach W and Nirenberg H 1982 The genusFusarium - A pictorial atlas. Mitt. BBA 209, 406 pp.Google Scholar
  22. Glauninger J, Langer I, Shala-Mayrhofer V, Lanzer B, Liebhard P 2002 Untersuchungen zur Beikrautflora und zu ausgewählten Pflanzenschutzproblemen bei unterschiedlicher Bodenbearbeitung.In Auswirkungen konservierender Bodenbearbeitungsmaßnahmen auf das Auftreten von Pflanzenkrankheiten, Schädlingen und Beikräutern in österreichischen Fruchtfolgesystemen. Eds. S Steinkellner, J Glauninger, I Langer, V Shala-Mayrhofer, P Liebhard, P Schausberger, G Grabenweger, B Lanzer and E Lawson-Balagbo. pp. 86–103. Abschlußbericht Forschungsprojekt, Nr. 1181, im Auftrag des BM:LFUW.Google Scholar
  23. Kandeler E and Böhm K E 1996 Temporal dynamics of microbial biomass, xylanase activity, N-mineralisation and potential nitrification in different tillage systems. Appl. Soil Ecol. 4, 181–191.CrossRefGoogle Scholar
  24. Kandeler E, Tscherko D and Spiegel H 1999 Long-term monitoring of microbial biomass, N mineralisation and enzyme activities of a Chernozem under different tillage treatments. Biol. Fertil Soils 28, 343–351.CrossRefGoogle Scholar
  25. Lew H, Adler A, Edinger W, Brodacz W, Kiendler E and Hinterholzer J 2001 Fusarien und ihre Toxine bei Mais in Österreich. Bodenkultur 52, 199–207.Google Scholar
  26. Liebhard P 1993a Einfluß der Primärbodenbearbeitung auf Textur und organische Substanz von Ackerböden im oberösterreichischen Zentralraum (Teil 1). Bodenkultur 44, 199–210.Google Scholar
  27. Liebhard P 1993b Einfluß der Primärbodenbearbeitung auf pH-Wert, Calcium-, Phosphat- und Kaliumgehalt von Ackerböden im oberösterreichischen Zentralraum (Teil 2). Bodenkultur 44, 303–315.Google Scholar
  28. Liebhard P 1994 Einfluß der Primärbodenbearbeitung auf Lagerungsdichte, Porenvolumen und Porengrößenverteilung von Ackerböden im oberösterreichischen Zentralraum (Teil 3). Bodenkultur 45, 125–138.Google Scholar
  29. Liebhard P, Eitzinger J and Klaghofer E 1994 Einfluß der Primärbodenbearbeitung auf Infiltration und Bodenwasservorrat im oberösterreichischen Zentralraum (Teil 4). Bodenkultur 45, 297–311.Google Scholar
  30. Lipps P E and Deep I W 1991 Influence of tillage and crop rotation on yield, stalk rot, and recovery ofFusarium andTrichoderma spp. from corn. Plant Dis. 75, 828–833.CrossRefGoogle Scholar
  31. McMullen M P and Stack R W 1983 Effects of isolation techniques and media on the differential isolation ofFusarium species. Phytopathology 73, 458–462.CrossRefGoogle Scholar
  32. Mesterházy Á, Bartók T, Mirocha C G and Komoróczy 1999 Nature of wheat resistance toFusarium head blight and the role of deoxynivalenol for breeding. Plant Breed. 118, 97–110.Google Scholar
  33. Miller J D, Culley J, Fraser K, Hubbard S and Meloche F 1998 Effect of tillage practice onFusarium head blight of wheat. Can. J. Plant Pathol. 20, 95–103.Google Scholar
  34. Mitchell J E 1979 The dynamics of the inoculum potential of populations of soil-borne plant pathogens in the soil ecosystem.In Soil-borne Plant Pathogens. Eds. B Schippers and W Gams. pp. 3–20. Academic Press, London, New York, San Francisco.Google Scholar
  35. Nash S M, Christou T and Snyder W C 1961 Existence ofFusarium solani f.phaseoli as chlamydospores in soil. Phytopathology 51, 308–312.Google Scholar
  36. Nelson P E, Toussoun T A and Marasas W F O 1983Fusarium species. An illustrated manual for identification. The Pennsylvania State University Press, University Park and London. 193 pp.Google Scholar
  37. Nirenberg H I 1976 Untersuchungen über die morphologische und biologische Differenzierung in derFusarium-Sektion Liseola. Mitt. a. d. Biol. Bundesanst. 169, 1–117.Google Scholar
  38. Obst A and Paul V H 1993 Krankheiten und Schädlinge des Getreides. Verlag Th. Mann, Gelsenkirchen-Buer. 184 p.Google Scholar
  39. Parkinson D 1973 Techniques for the study of soil fungi. Bull. Ecol. Res. Comm. (Stockholm) 17, 29–36.Google Scholar
  40. Reid L M, Zhu X and Ma B L 2001 Crop rotation and nitrogen effects on maize susceptibility to gibberella (Fusarium graminearum) ear rot. Plant Soil 237, 1–14.CrossRefGoogle Scholar
  41. Rodriguez-Molina M C, Tello-Marquina J C, Torres-Vila L M and Bielza-Lino P 2000 Micro-scale systematic sampling of soil: heterogeneity in populations ofFusarium oxysporum, F. solani, F. roseum andF. moniliforme. J. Phytopathol. 148, 609–614.CrossRefGoogle Scholar
  42. Rush C M, Mihail J D and Singleton L L 1992 Introduction.In Methods for research on soilborne phytopathogenic fungi. Eds. L L Singleton, J D Mihail and C M Rush. pp. 3–16. APS Press, St. Paul, Minnesota.Google Scholar
  43. Shala-Mayrhofer V and Glauninger J 2002 Einfluss von Bodenbearbeitungsverfahren auf den Fusariumbefall von Maiskolben. Bericht ALVA-Jahrestagung 2002, 27. – 29. Mai 2002 Klosterneuburg, 273–274.Google Scholar
  44. Steinkellner S, Shala-Mayrhofer V, Langer I 2002 Influence of tillage onFusarium spp. in different crop rotation systems. Mycotoxin Research. Proc. 24th Mykotoxin-Workshop Berlin, 3–5 June 2002, Vol. 1, 11–14.Google Scholar
  45. Stotzky G 1997 Soils as an environment for microbial life.In Modern soil microbiology. Eds. J D van Elsas, J T Trevors, and E M H Wellington. pp. 1–20. Marcel Dekker Inc. New York.Google Scholar
  46. Swan L J, Backhouse D and Burgess L W 2000 Surface soil moisture and stubble management practice effects on the progress of infection of wheat byFusarium pseudograminearum. Aust. J. Exp. Agric. 40, 693–698.CrossRefGoogle Scholar
  47. Thrane U 2001 Developments in the taxonomy ofFusarium species based on secondary metabolites. InFusarium. Eds. B A Summerell, J F Leslie, D Backhouse, W L Bryden and L W Burgess. pp. 29–49. APS Press, St. Paul, Minnesota.Google Scholar
  48. Walker S L, Leath S, Hagler W M and Murphy J P 2001 Variation among isolates ofFusarium graminearum associated with Fusarium head blight in North Carolina. Plant Dis. 85, 404–410.Google Scholar
  49. Weber R, Hrynczuk B, Runowska-Hrynzuk B and Kita W 2001 Influence of the mode of tillage on diseases of culm base of some winter wheat varieties, oats and spring wheat. J. Phytopathol. 149, 185–188.CrossRefGoogle Scholar
  50. Weingärtner J, Krska R, Praznik W, Grasserbauer M and Lew H. 1997 Use of Mycosep multifunctional clean-up columns for the determination of trichothecenes in wheat by electrone capture gas-chromatography. Fresenius J. Anal. Chem. 357, 1206–1210.Google Scholar
  51. Windels C E 1992Fusarium.In Methods for research on soilborne phytopathogenic fungi. Eds. L L Singleton, L D Mihail and C M Rush. pp. 115–128. APS Press, St. Paul, Minnesota.Google Scholar
  52. Wildermuth G B, Thomas G A, Radford B J, McNamara R B and Kelly A 1997 Crown rot and common root rot in wheat grown under different tillage and stubble treatments in southern Queensland, Australia. Soil Till. Res. 44, 211–224.CrossRefGoogle Scholar
  53. Yi C, Kaul H P, Kübler E, Schwadorf K and Aufhammer W 2001 Head blight (Fusarium graminearum) and deoxynivalenol concentration in winter wheat as affected by pre-crop, soil tillage and nitrogen fertilization. J. Plant Dis. Prot. 108, 217–230.Google Scholar
  54. Yi C, Kaul H P, Kübler E and Aufhammer W 2002 Populations ofFusarium graminearum on crop residues as affected by incorporation depth, nitrogen and fungicide application. J. Plant Dis. Prot. 109, 252–263.Google Scholar
  55. ZAMG (Central Institute of Meteorology and Geodynamics, Vienna) 2003 http://www.zamg.ac.atGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.Department of Applied Plant Sciences and Plant Biotechnology, Institute of Plant ProtectionUniversity of Natural Resources and Applied Life SciencesViennaAustria

Personalised recommendations