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Plant and Soil

, Volume 279, Issue 1–2, pp 287–296 | Cite as

Effect of Cu Toxicity on Growth of Cowpea (Vigna unguiculata)

  • Peter M. Kopittke
  • Neal W. Menzies
Article

Abstract

Accurate determination of the rhizotoxicity of Cu in dilute nutrient solutions is hindered by the difficulty of maintaining constant, pre-determined concentrations of Cu (micromolar) in solution. The critical Cu2+ activity associated with a reduction in the growth of solution-grown cowpea (Vigna unguiculata (L.) Walp. cv. Caloona) was determined in a system in which Cu was maintained constant through the use of a cation exchange resin. The growth of roots and shoots was found to be reduced at solution Cu2+ activities ≥1.7 μM (corresponding to 90% maximum growth). Although root growth was most likely reduced due to a direct Cu2+ toxicity, it is considered that the shoot growth reduction is attributable to a decrease in tissue concentrations of K, Ca, Mg, and Fe and the formation of interveinal chlorosis. At high Cu2+ activities, roots were brown in color, short and thick, had bent root tips with cracking of the epidermis and outer cortex, and had local swellings behind the roots tips due to a reduction in cell elongation. Root hair growth was reduced at concentrations lower than that which caused a significant reduction in overall root fresh weight.

Keywords

buffer copper toxicity heavy metal ion exchange resin root symptoms 

Abbreviations

CDTA

cyclohexane-1,2-diaminetetra-acetic acid

DI

deionized

EC

electrical conductivity

I

ionic strength

ICPAES

inductively coupled plasma atomic emission spectrometry

ICPMS

inductively coupled plasma mass spectrometry

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Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.School of Land and Food SciencesThe University of QueenslandSt. LuciaAustralia

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