Plant and Soil

, Volume 272, Issue 1–2, pp 77–86 | Cite as

Selenium – an antioxidative protectant in soybean during senescence

  • M. Djanaguiraman
  • D. Durga Devi
  • Arun K. ShankerEmail author
  • J. Annie Sheeba
  • U. Bangarusamy


Selenium (Se) is regarded as an antioxidant in animals and plants, even though considered as non-essential element in plants. To test its ability to counteract senescence related oxidative stress in soybean a pot culture experiment was conducted. The soybean plant was sprayed with sodium selenate (50 ppm) at 78 days after sowing (DAS). Soybean leaves were harvested at 80 and 90 DAS for analysis of oxidant production and antioxidative enzymes activity. Se positively promoted growth and acted as antioxidant by inhibiting lipid peroxidation and per cent injury of cell membrane. The antioxidative effect was associated with an increase in superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) enzymes activity. Significant increase in antioxidant enzyme activity was positively related to Se content. The decrease in antioxidative enzymes at 90 DAS was much faster in control plants than Se-sprayed plants. The reduction in SOD and GSH-Px may be associated with senescence-induced oxidative burst.


antioxidant enzymes selenium senescence oxidants 


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  1. Arnon, DI. 1949Copper enzyme in isolated chloroplasts: Polyphenol oxidase in Beta vulgarisPlant Physiol.24115Google Scholar
  2. Bates, LS, Waldreu, RP., Teak, TD. 1973Rapid determination of free proline for water stress studiesPlant Soil.39205207Google Scholar
  3. Behne, D, Kyriakopoulos, A, Kalcklosch, M, Weiss-Nowak, C, Pfeifer, H, Gessner, H., Hammel, C. 1997Two new selenoproteins found in the prostatic glandular epithelium and in the spermatid nucleiBiomed. Environ. Sci.10340345PubMedGoogle Scholar
  4. Behra, TH, Panda, SK., Patra, HK. 1999Chromium ion induced lipid peroxidation in developing wheat seedlings: role of growth hormonesIndian J. Plant Physiol.4236238Google Scholar
  5. Berlett, BS., Stadtman, ER. 1997Protein oxidation in Aging, Disease and Oxidative stressJ. Biol. Chem.2722031320316PubMedGoogle Scholar
  6. Berry, MJ, Banu, L, Chen, Y, Mandal, SJ, Kieffer, JD, Harney, JW., Larsen, PR. 1991Recognition of UGA as a selenocysteine codon in Type I deiodinase requires sequence in the 3′ untranslated regionNature353273276PubMedGoogle Scholar
  7. Beyer, WF., Fridovich, I. 1987Assaying for superoxide dismutase activity: some large consequences of minor changes in conditionsAnal. Biochem.161559566PubMedGoogle Scholar
  8. Block, G, Patterson, B., Subar, A. 1992Fruit, Vegetables and cancer prevention: a review of the epidemioloigcal evidenceNutr. Cancer.18129PubMedGoogle Scholar
  9. Chaitanya, KSK., Naithani, SC. 1994Role of superoxide, lipid peroxidation and superoxide dismutase in membrane perturbation during loss of viability in seeds of Shorea robustaGaertn. f. New Phytol126623627Google Scholar
  10. Chowdhury, SR., Choudhuri, MA. 1985Hydrogen peroxide metabolism as an index of water stress tolerance in jutePhysiol. Plant.65476480Google Scholar
  11. Dhindsa, RS, Dhindsa, PP., Thorpe, TA. 1981Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decrease levels of SOD and catalaseJ. Exp. Bot.3293101Google Scholar
  12. Djanaguiraman, M, Durga Devi, D, Shanker, AK., Bangarusamy, U. 2004Influence of selenium on antioxidant enzymes and yield of soybeanJ. Agric. Res Manage.314(Article in press)Google Scholar
  13. Epp, O, Ladenstein, R., Wendel, A. 1983The refined structure of the selenoenzyme glutathione peroxidase at 0.2 nm resolutionEur. J. Biochem.1335169PubMedGoogle Scholar
  14. Flohe L., Gunzler W A 1984 Assays of glutathione peroxidase. In Methods in Enzymology. Ed. L. Packer Vol. 105, pp. 114–121. Academic Press, New YorkGoogle Scholar
  15. Foyer, CH., Noctor, G. 2000Oxygen processing in photosynthesis: regulation and signaling Tanksley Review No. 112New Phytol.146359388Google Scholar
  16. Gladyshev, VN, Jeang, KT, Wootton, JC., Hatfield, DL. 1998A new human selenium-containing protein: purification, characterization and cDNA sequenceJ. Biol Chem.27389108915PubMedGoogle Scholar
  17. Golubkina, NA, Skalnyi, AV, Sokolov, YA., Shchelkunov, LF. 2002Selenium in Medicine and EcologyKMKMoscowGoogle Scholar
  18. Grove, A, Sabat, SC., Mohanty, P. 1986Effect of temperature on photosynthetic activities of senescent detached wheat leavesPlant Cell Physiol.27117126Google Scholar
  19. Hartikainen, H., Xue, T. 1999The promotive effect of selenium on plant growth as trigged by ultraviolet irradiationJ. Environ. Qual.2812721275Google Scholar
  20. Hartikainen, H, Xue, T., Piironen, V. 2000Selenium as an anti-oxidant and pro-oxidant in ryegrassPlant Soil.225193200Google Scholar
  21. Heath, RL., Packer, L. 1968Photoperoxidation in isolated chloroplasts: kinetics and stoichiometry of fatty acid peroxidationArch. Biochem. Biophys.125189198CrossRefPubMedGoogle Scholar
  22. Hu, Q, Pan, G., Zhu, J. 2002Effect of fertilization on selenium content of tea and the nutritional function of Se-enriched tea in ratsPlant Soil.2389195Google Scholar
  23. Jeong, DW, Kim, TS, Chung, YW, Lee, BJ., Kim, IY. 2002Selenoprotein W is a glutathione-dependent antioxidant in vivoFEBS Lett.517225228PubMedGoogle Scholar
  24. Kar, M., Feirabend, J. 1984Metabolism of activated oxygen in detached wheat and rye leaves and its relevance to the initiation of senescencePlanta160385391Google Scholar
  25. Kumpulainen, J, Raittila, AM, Lehto, J., Koivistoinen, P. 1983Electrothermal atomic absorption spectrometric determination of selenium in foods and dietsJ. Assoc. Official Anal. Chem.6611291135Google Scholar
  26. Lu, C., Zhang, J. 1998Modifications in photosystem II photochemistry in senescent leaves of maize plantsJ. Exp. Bot.4916711679Google Scholar
  27. Lu, C, Liu, Q, Zhang, J., Kuang, T. 2001Characterization of photosynthetic pigment composition, photosystem II photochemistry and thermal energy dissipation during leaf senescence of wheat plants grown in the fieldJ. Exp. Bot.5218051810PubMedGoogle Scholar
  28. Lu, SC. 1999Regulation of hepatic glutathione synthesis: current concepts and controversiesFASEB J.1311691183PubMedGoogle Scholar
  29. Mates, JM. 2000Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicologyToxicology15383104PubMedGoogle Scholar
  30. Matysik, J, Alia, A, Bhalu, B., Mohanty, P. 2002Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plantsCurr. Sci.821525532Google Scholar
  31. Maxwell, K., Johnson, GN. 2000Chlorophyll fluorescence – A practical guideJ. Exp. Bot.51659668CrossRefPubMedGoogle Scholar
  32. Noctor, G., Foyer, CH. 1998Ascorbate and glutathione: keeping active oxygen under controlAnn. Rev. Plant Physiol. Plant Mol. Biol.49249279Google Scholar
  33. Nooden L D., Thompson J E 1985 Aging and senescence in plants. In: Handbook of the biology of Aging, Ed. C E Finch, R C Adelman, G M Martin and E J. Masoro pp. 105. Van Nostrand Reinhold, New YorkGoogle Scholar
  34. Nooden, LD. 1984Integration of soybean pod development and monocarpic senescencePhysiol. Plant.62273284Google Scholar
  35. Nooden, LD. 1988Whole plant senescenceNooden, LDLeopold, AC. eds. Senescence and aging in plants.Academic PressSan Diego391439Google Scholar
  36. Nooden, LD, Kahanak, GM., Okatan, Y. 1979Prevention of monocarpic senescence in soybeans with auxin and cytokinin: An antidote for self destructionScience206841843Google Scholar
  37. Okuda, T, Matsuda, Y, Yamanaka, A., Sagisaka, S. 1991Abrupt increase in the level of hydrogen peroxide in leaves of winter wheat is caused by cold treatmentPlant Physiol.9712651267Google Scholar
  38. Pallud, S, Ramauge, MA, Gavaet, JM, Croteau, W, Pierre, M, Courtin, F., Germain, DLS. 1997Expression of type II iodythyronine deiodinase in cultured rat astrocytes is selenium-dependentJ. Biochem. Chem.2721810418110Google Scholar
  39. Panigrahi, PK., Biswal, UC. 1979Ageing of chloroplasts. In vitro – Quantitative analysis of the degradation of pigments, proteins and nucleic acidsPlant Cell Physiol.20775779Google Scholar
  40. Peiser, GD., Yang, SF. 1978Chlorophyll destruction in the presence of bisulfite and linoleic acid hydroperoxidesPhytochemistry177984Google Scholar
  41. Prinz, WA, Aslund, F, Holmgren, A., Beckwith, J. 1997The role of the thioredoxin and glutaredoxin pathways in reducing protein disulfide bonds in the E. coli cytoplasmJ. Biol. Chem.2721566115667PubMedGoogle Scholar
  42. Ramauge, M, Pallud, S, Esfandiari, A, Gavaret, J, Lennon, A, Pierre, M., Courtin, F. 1996Evidence the type III iodothhyronine deiodinase in rat astrocyte is a selenoproteinEndocrinology13730213025Google Scholar
  43. Rotruck, JT, Pope, AH, Ganther, HE, Swanson, AB, Hafeman, DG., Hoekstra, WG. 1973Selenium: biochemical role as a component of glutathione peroxidaseScience179588590PubMedGoogle Scholar
  44. Samantary, S. 2002Biochemical responses of Cr-tolerant and Cr-sensitive mung bean cultivars grown on varying levels of chromiumChemosphere4710651072PubMedGoogle Scholar
  45. Seppanen, M, Turakainen, M., Hartikainen, H. 2003Selenium effects on oxidative stress in potatoPlant Sci.165311319CrossRefGoogle Scholar
  46. Shanker, AK, Djanaguiraman, M, Sudhagar, R, Chandrashekar, CN., Pathmanabhan, G. 2004Differential antioxidative response of ascorbate glutathione pathway enzymes and metabolites to chromium speciation stress in green gram (Vigna radiata (L.) R. Wilczek. cv CO 4) rootsPlant Sci.16610351043Google Scholar
  47. Stadtman, ER. 1992Protein oxidation and agingScience25712201224PubMedGoogle Scholar
  48. Stadtman, TC. 1990Selenium biochemistryAnnu. Rev. Biochem.59111127PubMedGoogle Scholar
  49. Tamura, T., Stadtman, TC. 1996A new selenoprotein from human lung adenocarcinoma cells : purification, properties, and thioredoxin reductase activityProc. Natl. Acad. Sci. USA9310061011PubMedGoogle Scholar
  50. Terry, N., Zayed, AM. 1994Selenium volatilization by plantsFrankenberger, WT,JrBenson, S. eds. Selenium in Environment.Marcel Dekker Inc.New York343367Google Scholar
  51. Terry, N, Carlson, C, Raab, TK., Zayed, AM. 1992Rates of selenium volatilization among crop speciesJ. Environ. Qual.21341344Google Scholar
  52. Thomas, H, Ougham, H., Hortensteiner, S. 2001Recent advances in the cell biology of chlorophyll catabolismAdv. Bot. Res.35152Google Scholar
  53. Thompson, JE, Legge, RI., Barber, R 1987The role of free radicals in senescence and woundingNew Physiol.105317344Google Scholar
  54. Van Kooten, O., Snell, JFH. 1990The use of chlorophyll fluorescence nomenclature in plant stress physiologyPhotosynth. Res.25147150CrossRefGoogle Scholar
  55. Xue, T., Hartikainen, H. 2000Association of antioxidative enzymes with the synergistic effect of selenium and UV irradiation in enhancing plant growthAgric. Food Sci. Finland9177186Google Scholar
  56. Xue, T, Hartikainen, H., Piironen, V. 2001Antioxidative and growth–promoting effect of selenium on senescing lettucePlant Soil.2375561Google Scholar
  57. Xue, T, Hou, S., Tan, J. 1993The antioxidative function of selenium in higher plants: I The inhibitive effect of selenium on lipid peroxidation and its enzymatic mechanismChinese Sci. Bull.38274277Google Scholar
  58. Xue, T, Hou, S., Tan, J. 1993The antioxidative function of selenium in higher plants: II Non-enzymatic mechanismsChinese Sci. Bull.38356358Google Scholar
  59. Yokota, A, Shigeoka, S, Onishi, T., Kitaoka, S. 1988Selenium as inducer of glutathione peroxidase in low-CO2 grown Chlamydomonas reinhardtiiPlant Physiol.86649651Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • M. Djanaguiraman
    • 1
  • D. Durga Devi
    • 1
  • Arun K. Shanker
    • 2
    Email author
  • J. Annie Sheeba
    • 1
  • U. Bangarusamy
    • 1
  1. 1.Department of Crop PhysiologyTamil Nadu Agricultural UniversityCoimbatoreIndia
  2. 2.National Research Centre for Agroforestry Pahuj DamJhansiIndia

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