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Plant and Soil

, Volume 272, Issue 1–2, pp 29–40 | Cite as

Arbuscular mycorrhiza decreases cadmium phytoextraction by transgenic tobacco with inserted metallothionein

  • M. JanouškováEmail author
  • D. Pavlíková
  • T. Macek
  • M. Vosátka
Article

Abstract

The effect of arbuscular mycorrhiza (AM) on the phytoextraction efficiency of transgenic tobacco with increased ability to tolerate and accumulate cadmium (Cd) was tested in a pot experiment. The tobacco plants bearing the yeast metallothionein CUP1 combined with a polyhistidine cluster were compared to non-transgenic tobacco of the same variety at four Cd concentrations in soil, non-inoculated or inoculated with two isolates of the AM fungus Glomus intraradices. Mycorrhizal inoculation improved the growth of both the transgenic and non-transgenic tobacco and decreased Cd concentrations in shoots and root to shoot translocation. Differences were found between the two AM fungal isolates: one isolate supported more efficient phosphorus uptake and plant growth in the soil without Cd addition, while the other isolate alleviated the inhibitory effect of cadmium on plant growth. The resulting effect of inoculation on Cd accumulation was dependent on Cd level in soil and differed between the more Cd tolerant transgenic plants and the less tolerant non-transgenic plants. Mycorrhiza mostly decreased the phytoextraction efficiency of transgenic plants while increased that of non-transgenic plants at Cd levels in soil inhibitory to tobacco growth. Mechanisms of the observed effects of inoculation on growth and Cd uptake are discussed as well as the possible implications of the results for the exploitation of AM in phytoextraction of heavy metals from contaminated soils.

Keywords

CUP1 gene Glomus heavy metals soil microflora 

Abbreviations

AM

arbuscular mycorrhiza

ERM

extraradical mycelium

HisCUP

transgenic tobacco

HM

heavy metal

WSC

non-transgenic tobacco

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Copyright information

© Springer 2005

Authors and Affiliations

  • M. Janoušková
    • 1
    Email author
  • D. Pavlíková
    • 2
  • T. Macek
    • 3
  • M. Vosátka
    • 1
  1. 1.Department of Mycorrhizal Symbioses, Institute of BotanyAcademy of Sciences of the Czech RepublicCzech Republic
  2. 2.Department of Agrochemistry and Plant NutritionCzech University of AgriculturePragueCzech Republic
  3. 3.Department of Natural Products, Institute of Organic Chemistry and BiochemistryAcademy of Sciences of the Czech RepublicPragueCzech Republic

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