Advertisement

Plant and Soil

, Volume 270, Issue 1, pp 257–267 | Cite as

A comparative study between two citrus rootstocks: Effect of nitrate on the root morpho-topology and net nitrate uptake

  • Agostino Sorgonà
  • Maria Rosa Abenavoli
  • Giovanni Cacco
Article

Abstract

Root morpho-topology and net nitrate uptake of two citrus seedlings, Volkamer Lemon and Carrizo Citrange, grown at two nitrogen supplies (NO3-N 5 μM and 1000 μM, respectively) were studied. Root morphological and topological parameters were gauged by an image-specific analysis system (WinRHIZO). Net nitrate uptake was estimated using the nitrate depletion method. The main findings showed that Carrizo seedlings had a dichotomous branching root system characterized by high root tip numbers and long 2 nd order lateral roots. Conversely, Volkamer root systems had a herringbone structure with a long tap root and 1 st order lateral root. Nitrate treatment did not seem to affect the pattern of the two genotypes, except for the 2 nd order lateral roots (Carrizo more than Volkamer) and root/shoot ratio and root mass ratio (Volkamer more than Carrizo) that were significantly different at low nitrate supply. Nitrate treatments induced a diverse net nitrate uptake regulation between citrus rootstocks. Indeed, at low nitrate supply, Carrizo showed a more efficient nitrate acquisition process in terms of: 1) higher net nitrate uptake maximum of the inducible high affinity transport system or ‘full induction’ (A), (2) higher cumulative nitrate uptake (A t ) and (3) lower t1 parameter defined as the half time of the net nitrate uptake rate of the inducible transport system during the induction phase, compared to Volkamer. Conversely, at the high nitrate level, only the genotypical difference of the t1 parameter was maintained. The results suggested that, at the low nitrate level, the morphological root traits such as higher 2 nd order lateral roots and greater root tip numbers of the Carrizo compared with Volkamer seedlings, enhance the capacity to absorb nitrate from nutrient solution.

Keywords

Carrizo Citrange nitrate uptake root morphology root topology Volkamer Lemon 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abenavoli, M R, De Santis, C, Sidari, M, Sorgonà, A, Cacco, G 2001Influence of coumarin on the net nitrate uptake in durum wheatNew Phytologist.150619627CrossRefGoogle Scholar
  2. Aslam, M, Travis, R L, Huffaker, R C 1992Comparative kinetics and reciprocal inhibition of nitrate and nitrite uptake in roots of uninduced and induced barley seedlingsPlant Physiol.9911241133PubMedGoogle Scholar
  3. Bell, D L, Sultan, S E 1999Dynamic phenotypic plasticity for root growth in Polygonum: a comparative studyAm. J. Bot.86807819PubMedGoogle Scholar
  4. Brady, D J, Wenzel, C L, Fillery, I R P, Gregory, P J 1995Root growth and nitrate uptake by wheat (Triticum aestivum L.) following wetting of dry surface soilJ. Exp. Bot.46557564Google Scholar
  5. Carpenter, S R, Caraco, N F, Correll, D L, Howarth, R W, Sharply, A N, Smith, V H 1998Nonpoint pollution of surface waters with phopshorus and nitrogenEcol. Applic.8559568Google Scholar
  6. Casper, B R, Jackson, R B 1997Plant competition undergroundAnn. Rev. of Ecol. and System.28545570CrossRefGoogle Scholar
  7. Castle, W S, Youtsey, C O 1977Root system characteristics of citrus nursery treesProc. Florida State Horticult. Soc.903944Google Scholar
  8. Cataldo, D A, Haroon, M, Schrader, L E, Youngs, V L 1975Rapid colorimetric determination in plant tissue bi nitration of salicylic acidCommun. Soil Sci. Plant Anal.67180Google Scholar
  9. Cerezo, M, García-Augustín, P, Serna, M D, Primo-Millo, E. 1997Kinetics of nitrate uptake by Citrusseedlings and inhibitory effects of salinityPlant Sci.126105112Google Scholar
  10. Cerezo, M, Flors, V, Legaz, F, García-Augustín, P. 2000Characterization of the low affinity transport system for NO 3 uptake by CitrusrootsPlant Sci.16095104PubMedGoogle Scholar
  11. Colmer, T D, Bloom, A J 1998A comparison of NH 4 + and NO 3 net fluxes along roots of rice and maizePlant Cell Environ.21240246Google Scholar
  12. Crawford, N M 1995Nitrate: nutrient and signal for plant growthPlant Cell7859868PubMedGoogle Scholar
  13. Davies, F S, Albrigo, L G 1994CitrusWallingfordOxon UKGoogle Scholar
  14. Fitter, A H, Stickland, T R, Harvey, M L, Wilson, G W 1991Architectural analysis of plant root systems 1. Architectural correlates of exploitation efficiencyNew Phytologist118375382Google Scholar
  15. Fitter, A H, Stickland, T R 1991Architectural analysis of plant root systems 2. Influence of nutrient supply on architecture in contrasting plant speciesNew Phytologist118383389Google Scholar
  16. Forde, B G, Clarkson, D T 1999Nitrate and ammonium nutrition of plants: physiological and molecular perspectivesAdv. Bot. Res.30190Google Scholar
  17. Forde, B G, Lorenzo, H 2001The nutritional control of root developmentPlant Soil2325168Google Scholar
  18. Glimskar, A 2000Estimates of root system topology of five plant species grown at steady-state nutritionPlant Soil227249256Google Scholar
  19. Goldsmith, J, Livoni, J P, Norberg, C L, Segel, I H 1973Regulation of nitrate reductase in Penicillium chrysogenumby ammonium ionPlant Physiology.52362367Google Scholar
  20. Hoagland, D R, Arnon, D I 1950The water-culture method for growing plants without soilCalif. Agricult. Exp. Station Circ.34732Google Scholar
  21. Hodge, A, Stewart, J, Robinson, D, Griffiths, B S, Fitter, A H 1998Root proliferation, soil fauna and plant nitrogen capture from nutrient-rich patches in soilNew Phytologist139479494Google Scholar
  22. Hodge, A, Robinson, D, Griffiths, B S, Fitter, A H 1999Why plants bother: root proliferation results in increased nitrogen capture from an organic patch when two grasses competePlant Cell Environ.22811820Google Scholar
  23. Hole, D J, Emran, A M, Fares, Y, Drew, M C 1990Induction of nitrate transport in maize roots, and kinetics of influx, measured with nitrogen-13Plant Physiol.93642647Google Scholar
  24. Imsande, J, Touraine, B 1994N demand and the regulation of nitrate uptakePlant Physiol.10537PubMedGoogle Scholar
  25. Kronzucker, H J, Glass, A D M, Siddiqui, M Y 1995Nitrate induction in spruce: an approach using compartmental analysisPlanta196683690Google Scholar
  26. Lainè, P, Ourry, A, Boucaud, J 1995Shoot control of nitrate uptake rates by roots of Brassica napus L.: Effects of localized nitrate supplyPlanta1967783Google Scholar
  27. Lazof, D B, Rufty, T W, Redinbaugh, M G 1992Localization of nitrate absorption and translocation within morphological regions of the corn rootPlant Physiol.10012511258Google Scholar
  28. Lynch, J P 1995Root architecture and plant productivityPlant Physiol.109713PubMedGoogle Scholar
  29. MacKown, C T, McClure, P R 1988Development of accelerated net nitrate uptake. Effects of nitrate concentration and exposure timePlant Physiol.87162166Google Scholar
  30. Mistrik, I 1997Nitrate uptake and assimilation by individual roots of Zea maysBiologia Bratislava.52567571Google Scholar
  31. Newman, E I, Andrews, R E 1973Uptake of phosphorus and potassium in relation to root growth and root densityPlant Soil384669Google Scholar
  32. Poling, E B 1977The effect of rootstocks on apple leaf mineral element compositionCornell UniversityIthaca, N.Y.MS thesisGoogle Scholar
  33. Press, W H, Teukosky, S A, Wetterling, W T, Flannery, B P 1992Numerical recipes in C. The art of scientific computing2Cambridge University PressCambridgeGoogle Scholar
  34. Rao-Theertham, P, Ito-Osamu, , Matsunaga-Ryoichi, Yoneyama-Tadakatsu 1997Kinetics of 15N-labelled nitrate uptake by maize (Zea mays L.) root segmentsSoil Sci. Plant Nutrit.43491498Google Scholar
  35. Sattelmacher, B, Gerendas, J, Thoms, K, Bruck, H, Bagdady, N H 1993Interaction between root growth and mineral nutritionEnviron. Exp. Bot.336373Google Scholar
  36. Siddiqi, M Y, Glass, A D M, Ruth, T J, Fernando, M 1989Studies of the regulation of nitrate influx by barley seedlings using 13NO 3 Plant Physiol.90806813Google Scholar
  37. Siddiqi, M, Glass, A D M, Ruth, T J, Rufty, T W 1990Studies of uptake of nitrate in barley. I. Kinetics of 13NO 3 influxPlant Physiol.9314261432Google Scholar
  38. Siebrecht, S, Mack, G, Tischner, R 1995Function and contribution of the root tip in the induction of NO 3 uptake along the barley root axisJ. of Exp. Bot.4616691676Google Scholar
  39. Sorgonà, A, Cacco, G. 2002Linking the physiological parameters of nitrate uptake with root morphology and topology in wheat (Triticum durum) and citrus (Citrus volkameriana) rootstockCan. J. Bot.80494503Google Scholar
  40. Sullivan, W M, Jiang, Z, Hull, R J 2000Root morphology and its relationship with nitrate uptake in kentucky bluegrassCrop Sci.40765772Google Scholar
  41. Tischner, R 2000Nitrate uptake and reduction in higher and lower plantsPlant Cell Environ.2310051024Google Scholar
  42. Vitousek, P M, Hedin, L O, Matson, P A, Fownes, J H, Neff, J 1997Within-system element cycles, input-output budgets, and nutrient limitationPace, M LGroffman, P M eds. Success, limitations and frontiers in ecosystem scienceSpringer-VerlagNew York432451Google Scholar
  43. Waisel, Y, Eshel, A 1992Differences in ion uptake among roots of various typesJ. Plant Nutrit.15945958Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Agostino Sorgonà
    • 1
  • Maria Rosa Abenavoli
    • 1
  • Giovanni Cacco
    • 1
  1. 1.Dipartimento di Bioteconologie per il Monitoraggio Agroalimentare ed AmbientaleUniversità degli Studi ‘Mediterranea’ di Reggio CalabriaFeo di Vito – Reggio CalabriaItalia

Personalised recommendations