Plant and Soil

, Volume 270, Issue 1, pp 223–232 | Cite as

Isolation and characterization of Azotobacter and Azospirillum strains from the sugarcane rhizosphere

  • N. Tejera
  • C. Lluch
  • M. V. Martìnez-Toledo
  • J. Gonzàlez-López


Bacteria with the ability to grow on nitrogen-free media and with nitrogenase activity under aerobic or microaerobic conditions were isolated from sugarcane roots collected from four different agricultural locations in Granada (Spain). Isolates were Gram negative rods and were identified as Azotobacter chroococcum and Azospirillum brasilense. Our results suggest that Azotobacter isolates do not have a particular affinity for sugarcane rhizospheres and that, on the contrary, Azospirillum isolates show specific association and perhaps endophytic colonization of sugarcane. However, obligate endophytes (Gluconacetobacter diazotrophicus) were not found in the apoplastic fluid of the stems and macerates extracts of sugarcane tissues with the procedure applied. Population of this microorganism might be in low number in the Spanish sugarcane varieties studied which is also discussed.


Azospirillum Azotobacter nitrogen fixation sugarcane 


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Copyright information

© Springer 2005

Authors and Affiliations

  • N. Tejera
    • 1
  • C. Lluch
    • 1
  • M. V. Martìnez-Toledo
    • 2
  • J. Gonzàlez-López
    • 2
  1. 1.Departamento de Fisiologìa Vegetal, Facultad de CienciasUniversidad de GranadaGranadaSpain
  2. 2.Instituto del AguaUniversidad de GranadaGranadaSpain

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