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(Z)-3-Hexen-1-ol accumulation enhances hyperosmotic stress tolerance in Camellia sinensis

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Abstract

Volatile components in fresh leaves are involved in the regulation of many stress responses, such as insect damage, fungal infection and high temperature. However, the potential function of volatile components in hyperosmotic response is largely unknown. Here, we found that 7-day hyperosmotic treatment specifically led to the accumulation of (Z)-3-hexen-1-ol, (E)-2-hexenal and methyl salicylate. Transcriptome and qRT-PCR analyses suggested the activation of linolenic acid degradation and methyl salicylate processes. Importantly, exogenous (Z)-3-hexen-1-ol pretreatment dramatically enhanced the hyperosmotic stress tolerance of tea plants and decreased stomatal conductance, whereas (E)-2-hexenal and methyl salicylate pretreatments did not exhibit such a function. qRT-PCR analysis revealed that exogenous ABA induced the expressions of related enzyme genes, and (Z)-3-hexen-1-ol could up-regulate the expressions of many DREB and RD genes. Moreover, exogenous (Z)-3-hexen-1-ol tremendously induced the expressions of specific LOX and ADH genes within 24 h. Taken together, hyperosmotic stress induced (Z)-3-hexen-1-ol accumulation in tea plant via the activation of most LOX, HPL and ADH genes, while (Z)-3-hexen-1-ol could dramatically enhance the hyperosmotic stress tolerance via the decrease of stomatal conductance and MDA, accumulation of ABA and proline, activation of DREB and RD gene expressions, and probably positive feedback regulation of LOXs and ADHs.

Key message

Hyperosmotic stress induced (Z)-3-hexen-1-ol accumulation in Camellia sinensis via the up-regulation of most LOX, HPL and ADH genes, while (Z)-3-hexen-1-ol could dramatically enhance the hyperosmotic stress tolerance via the decrease of stomatal conductance, accumulation of proline, activation of DREB and RD gene expressions, and probably positive feedback regulation of LOXs and ADHs.

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Abbreviations

ABA:

Abscisic acid

ADH:

Alcohol dehydrogenase

bp:

Base pair

DEG:

Differentially expressed gene

DREB:

Dehydration responsive element binding protein

DW:

Dry weight

GC–MS:

Gas chromatography-mass spectrometer

GLV:

Green leaf volatile

GO:

Gene ontology

HI:

Hexenal isomerase

HPL:

Hydroperoxide lyase

KEGG:

Kyoto encyclopedia of genes and genomes

LOX:

Lipoxygenase

qRT-PCR:

Quantitative real-time polymerase chain reaction

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Funding

This work was supported by the National Key R&D Program of China (2018YFD1000601) and Fundamental Research Funds for the Central Universities (2662016QD024).

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Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China

    Shuangling Hu, Qinghua Chen, Fei Guo, Mingle Wang, Hua Zhao, Yu Wang, Dejiang Ni & Pu Wang

  2. Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China

    Shuangling Hu, Qinghua Chen, Fei Guo, Mingle Wang, Hua Zhao, Yu Wang, Dejiang Ni & Pu Wang

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  1. Shuangling Hu
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  2. Qinghua Chen
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  5. Hua Zhao
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  8. Pu Wang
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Contributions

SH and PW designed the experiments. SH and QC performed the experiments. SH and PW wrote the manuscript. FG, MW, HZ, YW and DN revised the manuscript.

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Correspondence to Pu Wang.

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The data sets of raw sequencing reads have been deposited in the National Center for Biotechnology Information (NCBI) database and can be retrieved under the BioProject Accession Number PRJNA513275.

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Hu, S., Chen, Q., Guo, F. et al. (Z)-3-Hexen-1-ol accumulation enhances hyperosmotic stress tolerance in Camellia sinensis. Plant Mol Biol 103, 287–302 (2020). https://doi.org/10.1007/s11103-020-00992-2

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