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Proteomic analysis of the similarities and differences of soil drought and polyethylene glycol stress responses in wheat (Triticum aestivum L.)

  • Guibin Cui
  • Yanfeng Zhao
  • Jialing Zhang
  • Manning Chao
  • Kunliang Xie
  • Chao Zhang
  • Fengli Sun
  • Shudong Liu
  • Yajun XiEmail author
Article
  • 103 Downloads

Abstract

Key message

Our results reveal both soil drought and PEG can enhance malate, glutathione and ascorbate metabolism, and proline biosynthesis, whereas soil drought induced these metabolic pathways to a greater degree than PEG.

Abstract

Polyethylene glycol (PEG) is widely used to simulate osmotic stress, but little is known about the different responses of wheat to PEG stress and soil drought. In this study, isobaric tags for relative quantification (iTRAQ)-based proteomic techniques were used to determine both the proteomic and physiological responses of wheat seedlings to soil drought and PEG. The results showed that photosynthetic rate, stomatal conductance, intercellular CO2 concentration, transpiration rate, maximum potential efficiency of PS II, leaf water content, relative electrolyte leakage, MDA content, and free proline content exhibited similar responses to soil drought and PEG. Approximately 15.8% of differential proteins were induced both by soil drought and PEG. Moreover, both soil drought and PEG inhibited carbon metabolism and the biosynthesis of some amino acids by altering the accumulation of glyceraldehyde-3-phosphate dehydrogenase, ribulose-bisphosphate carboxylase, and phosphoglycerate kinase, but they both enhanced the metabolism of malate, proline, glutathione, and ascorbate by increasing the accumulation of key enzymes including malate dehydrogenase, monodehydroascorbate reductase, pyrroline-5-carboxylate dehydrogenase, pyrroline-5-carboxylate synthetase, ascorbate peroxidase, glutathione peroxidase, and glutathione S-transferase. Notably, the latter five of these enzymes were found to be more sensitive to soil drought. In addition, polyamine biosynthesis was specifically induced by increased gene expression and protein accumulation of polyamine oxidase and spermidine synthase under PEG stress, whereas fructose-bisphosphate aldolase and arginase were induced by soil drought. Therefore, present results suggest that PEG is an effective method to simulate drought stress, but the key proteins related to the metabolism of malate, glutathione, ascorbate, proline, and polyamine need to be confirmed under soil drought.

Keywords

Water deficit Osmotic stress Proline Glutathione Ascorbate Polyamine 

Abbreviations

ADH

Alcohol dehydrogenase

APX

Ascorbate peroxidase

DP

Differentially accumulated protein

G6PD

Glucose-6-phosphate dehydrogenase

GO

Gene ontology annotation

GPX

Glutathione peroxidase

GST

Glutathione S-transferase

MDH

Malate dehydrogenase

P5CD

Pyrroline-5-carboxylate dehydrogenase

PDHB

Pyruvate dehydrogenase

PGK

Phosphoglycerate kinase

ROS

Reactive oxygen species

ALDO

Fructose-bisphosphate aldolase

AsA

Ascorbate

FC

Field capacity

GAPA

Glyceraldehyde-3-phosphate dehydrogenase

GPI

Glucose-6-phosphate isomerase

GSH

Glutathione

IDH

Isocitrate dehydrogenase

MDHAR

Monodehydroascorbate reductase

P5CS

Pyrroline-5-carboxylate synthetase

PEG

Polyethylene glycol

POD

Peroxidase

Notes

Acknowledgements

YX thanks The National Key Basic Research Program, China (2017YFD0100706), Protection and Utilization of Germplasm Resources of Shaanxi Province, China (20171010000004), and Agriculture Technology Demonstration Project of Yangling, China (2017-TS-20) for financial support.

Author contributions

YX designed and directed this study as well as drafted and revised the manuscript. GC and YZ performed the experiments and analyzed the data as well as drafted and revised the manuscript. MC and JZ conducted the physiological and stress parameters determination. KX measured the gene expression of all selected proteins. FS, CZ and SL improved the data analysis and revised the manuscript.

Supplementary material

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© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Crop Stress Biology for Arid Areas, College of AgronomyNorthwest A&F UniversityYanglingChina
  2. 2.Seed Management Center of Shaanxi ProvinceXianChina

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