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Plant Molecular Biology

, Volume 100, Issue 1–2, pp 3–18 | Cite as

Gene silencing approaches through virus-based vectors: speeding up functional genomics in monocots

  • Ravi Kant
  • Indranil DasguptaEmail author
Review
  • 512 Downloads

Abstract

Key message

The design and use of existing VIGS vectors for revealing monocot gene functions are described and potential new vectors are discussed, which may expand their repertoire.

Abstract

Virus induced gene silencing (VIGS) is a method of transient gene silencing in plants, triggered by the use of modified viral vectors. VIGS has found widespread use in deciphering the functions of plant genes, mainly for dicots. In the last decade, however, its use in monocots has increased noticeably, involving not only previously described viruses for monocots, but also those described for dicots. Additional viruses have been modified for VIGS to bring a larger collection of monocots under the ambit of this method. For monocots, new methods of inoculation have been tried to obtain increased silencing efficiency. The issue of insert stability and duration of silencing have also been addressed by various research groups. VIGS has been used to unravel the functions of a fairly large collection of monocot genes. This review summarizes the above developments, bringing out some of the gaps in our understanding and identifies directions to develop this technology further in the coming years.

Keywords

Virus induced gene silencing RNAi Functional genomics Monocots Virus 

Notes

Acknowledgements

RK acknowledges the research fellowship from Indian Council of Medical Research, New Delhi. ID acknowledges the financial support from Department of Biotechnology, Government of India (Grant No. BT/AB/FG-1(PH-II)/2009), J. C. Bose Fellowship, University of Delhi R&D Grant, PURSE Grant and FIST infrastructure grant.

Author contributions

RK and ID conceived the review, both RK and ID wrote the revised review. Both RK and ID approved the final version.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest associated with this review.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Plant Molecular BiologyUniversity of Delhi South CampusNew DelhiIndia

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