Plant Molecular Biology

, Volume 98, Issue 6, pp 507–523 | Cite as

Transient induction of a subset of ethylene biosynthesis genes is potentially involved in regulation of grapevine bud dormancy release

  • Zhaowan Shi
  • Tamar Halaly-Basha
  • Chuanlin Zheng
  • Mira Weissberg
  • Ron Ophir
  • David W. Galbraith
  • Xuequn Pang
  • Etti OrEmail author


Key message

Transient increases in ethylene biosynthesis, achieved by tight regulation of transcription of specific ACC oxidase and ACC synthase genes, play a role in activation of grapevine bud dormancy release.


The molecular mechanisms regulating dormancy release in grapevine buds are as yet unclear. It has been hypothesized that its core involves perturbation of respiration which induces an interplay between ethylene and ABA metabolism that removes repression and allows regrowth. Roles for hypoxia and ABA metabolism in this process have been previously supported. The potential involvement of ethylene biosynthesis in regulation of dormancy release, which has received little attention so far, is now explored. Our results indicate that (1) ethylene biosynthesis is induced by hydrogen cyanamide (HC) and azide (AZ), known artificial stimuli of dormancy release, (2) inhibitors of ethylene biosynthesis and signalling antagonize dormancy release by HC/AZ treatments, (3) ethylene application induces dormancy release, (4) there are two sets of bud-expressed ethylene biosynthesis genes which are differentially regulated, (5) only one set is transiently upregulated by HC/AZ and during the natural dormancy cycle, concomitant with changes in ethylene levels, and (6) levels of ACC oxidase transcripts and ethylene sharply decrease during natural dormancy release, whereas ACC accumulates. Given these results, we propose that transient increases in ethylene biosynthesis prior to dormancy release, achieved primarily by regulation of transcription of specific ACC oxidase genes, play a role in activation of dormancy release.


Vitis vinifera ACC synthase (ACS) ACC oxidase (ACO) Bud Dormancy Ethylene Grapevine 



This research was supported by the United States-Israel Binational Agricultural Research and Development Fund (BARD Grant No. IS-4639-13 to EO, DG, and RO). We thank Felix Shaya from the metabolomics unit of ARO for ACC quantitation. We also thank the China Scholarship Council for joint PhD fellowships provided to Zhaowan Shi.

Author contributions

Conceptualization: ZS, TH-B, RO, DWG, EO; investigation: ZS, TH-B, CZ, MW; writing—original draft: ZS, TH-B, EO; writing—review & editing: RO, DWG, XP, EO.

Supplementary material

11103_2018_793_MOESM1_ESM.pptx (883 kb)
Supplementary material 1 (PPTX 883 KB)
11103_2018_793_MOESM2_ESM.xls (396 kb)
Supplementary material 2 (XLS 396 KB)


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Zhaowan Shi
    • 1
    • 2
  • Tamar Halaly-Basha
    • 1
  • Chuanlin Zheng
    • 1
  • Mira Weissberg
    • 1
  • Ron Ophir
    • 1
  • David W. Galbraith
    • 3
  • Xuequn Pang
    • 2
  • Etti Or
    • 1
    Email author
  1. 1.Institute of Plant Sciences, Department of Fruit Tree Sciences, Agricultural Research OrganizationVolcani CenterRishon LeZionIsrael
  2. 2.College of Life SciencesSouth China Agricultural UniversityGuangzhouChina
  3. 3.School of Plant Sciences and Bio5 InstituteUniversity of ArizonaTucsonUSA

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