Picea wilsonii transcription factor NAC2 enhanced plant tolerance to abiotic stress and participated in RFCP1-regulated flowering time
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Picea wilsonii transcription factor PwNAC2 enhanced plant tolerance to salt and drought stress through multiple signaling pathway and interacted with PwRFCP1 to participate in flowering regulation.
NAC is one of the largest transcription factor families in plants, however, its role is not yet fully understood. Here, we identified a transcription factor PwNAC2 in Picea wilsonii, which localized in nucleus with transcriptional activity in C-terminal region and can form homodimer by itself. Expression analysis by real-time PCR showed that PwNAC2 was induced by multiple abiotic stresses and phytohormones stimuli. PwRFCP1 (Resemble-FCA-contain-PAT1 domain), an interaction protein of PwNAC2 was screened via yeast two hybrid. Luciferase complementation assay confirmed the interaction in vivo and bimolecular fluorescence complementation assay showed the interaction in nucleus. PwNAC2 overexpression retarded Arabidopsis hypocotyls growth which is closely related to light, whereas promotion of hypocotyls growth by PwRFCP1 is independent on light. Under drought or salt treatment, overexpression of PwNAC2 in Arabidopsis showed more vigorous seed germination and significant tolerance for seedlings by ROS scavenging, reducing of membrane damage, slower water loss and increased stomatal closure. ABA or CBF-pathway marker genes were substantially higher in PwNAC2 transgenic Arabidopsis. Overexpression of PwRFCP1 promotes flowering in transgenic Arabidopsis, whereas PwNAC2 delayed flowering by altering the expression of FT, SOC1 and FLC. In addtioin, PwRFCP1 overexpression plants showed no higher tolerance to stress treatment than Col-0. Collectively, our results indicate that PwNAC2 enhanced plant tolerance to abiotic stress through multiple signaling pathways and participated in PwRFCP1-regulated flowering time.
KeywordsPicea wilsonii Transcription factor PwNAC2 PwRFCP1 Abiotic stress Flowering time
We thank Professor Dapeng Zhang (College of life sciences, Tsinghua University) for providing the PCM1205 and PBI121 vectors.
Hehua Zhang performed the experiments and wrote the manuscript; Xiaoyue Cui and Yuxiao Guo revised the manuscript; Chaobing Luo cloned PwNAC2 gene; Lingyun Zhang designed the experiment and revised the manuscript.
This work was supported by the grant from Agricultural Ministry of China (Grant No. 2016ZX08009-003-002).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
- Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399. https://doi.org/10.1146/annurev.arplant.55.031903.141701 CrossRefGoogle Scholar
- Faria JA, Reis PA, Reis MT, Rosado GL, Pinheiro GL, Mendes GC, Fontes EP (2011) The NAC domain-containing protein, GmNAC6, is a downstream component of the ER stress- and osmotic stress-induced NRP-mediated cell-death signaling pathway. BMC Plant Biol 11:129. https://doi.org/10.1186/1471-2229-11-129 CrossRefPubMedPubMedCentralGoogle Scholar
- He XJ, Mu RL, Cao WH, Zhang ZG, Zhang JS, Chen SY (2005) AtNAC2, a transcription factor downstream of ethylene and auxin signaling pathways, is involved in salt stress response and lateral root development. Plant J 44:903–916. https://doi.org/10.1111/j.1365-313X.2005.02575.x CrossRefPubMedGoogle Scholar
- Mendes GC, Reis PAB, Calil IP, Carvalho HH, Aragão FJL, Fontes EPB (2013) GmNAC30 and GmNAC81 integrate the endoplasmic reticulum stress- and osmotic stress-induced cell death responses through a vacuolar processing enzyme. Proc Natl Acad Sci USA 110:19627–19632. https://doi.org/10.1073/pnas.1311729110 CrossRefPubMedGoogle Scholar
- Porra R, Thompson W, Kriedemann P (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. BBA Biomembr 975:384–394. https://doi.org/10.1016/S0005-2728(89)80347-0 CrossRefGoogle Scholar
- Sakuraba Y, Kim YS, Han SH, Lee BD, Paek NC (2015) The Arabidopsis transcription factor NAC016 promotes drought stress responses by repressing AREB1 transcription through a trifurcate feed-forward regulatory loop involving NAP. Plant Cell 27:1771–1787. https://doi.org/10.1105/tpc.15.00222 CrossRefPubMedPubMedCentralGoogle Scholar
- Simpson GG, Laurie RE, Dijkwel PP, Quesada V, Stockwell PA, Dean C, Macknight RC (2010) Noncanonical translation initiation of the Arabidopsis flowering time and alternative polyadenylation regulator FCA. Plant Cell 22:3764–3777. https://doi.org/10.1105/tpc.110.077990 CrossRefPubMedPubMedCentralGoogle Scholar
- Tran LS et al (2004) Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter. Plant Cell 16:2481–2498. https://doi.org/10.1105/tpc.104.022699 CrossRefPubMedPubMedCentralGoogle Scholar
- Zhang T, Zhang D, Liu Y, Luo C, Zhou Y, Zhang L (2015) Overexpression of a NF-YB3 transcription factor from Picea wilsonii confers tolerance to salinity and drought stress in transformed Arabidopsis thaliana. Plant Physiol Biochem 94:153–164. https://doi.org/10.1016/j.plaphy.2015.05.001 CrossRefPubMedGoogle Scholar
- Zhao Y, Sun J, Xu P, Zhang R, Li L (2014b) Intron-mediated alternative splicing of wood-associated NAC transcription factor1b regulates cell wall thickening during fiber development in Populus species. Plant Physiol 164:765–776. https://doi.org/10.1104/pp.113.231134 CrossRefPubMedPubMedCentralGoogle Scholar
- Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273. https://doi.org/10.1146/annurev.arplant.53.091401.143329 CrossRefPubMedPubMedCentralGoogle Scholar