Plant Molecular Biology

, Volume 93, Issue 1–2, pp 209–225 | Cite as

Kelch-motif containing acyl-CoA binding proteins AtACBP4 and AtACBP5 are differentially expressed and function in floral lipid metabolism

  • Zi-Wei Ye
  • Jie Xu
  • Jianxin Shi
  • Dabing Zhang
  • Mee-Len Chye
Article

Abstract

Key message

We herein demonstrated two of the Arabidopsis acyl-CoA-binding proteins (ACBPs), AtACBP4 and AtACBP5, both function in floral lipid metabolism and they may possibly play complementary roles in Arabidopsis microspore-to-pollen development. Histological analysis on transgenic Arabidopsis expressing β-glucuronidase driven from the AtACBP4 and AtACBP5 promoters, as well as, qRTPCR analysis revealed that AtACBP4 was expressed at stages 11–14 in the mature pollen, while AtACBP5 was expressed at stages 7–10 in the microspores and tapetal cells. Immunoelectron microscopy using AtACBP4- or AtACBP5-specific antibodies further showed that AtACBP4 and AtACBP5 were localized in the cytoplasm. Chemical analysis of bud wax and cutin using gas chromatographyflame ionization detector and GC-mass spectrometry analyses revealed the accumulation of cuticular waxes and cutin monomers in acbp4, acbp5 and acbp4acbp5 buds in comparison to the wild type (Col-0). Fatty acid profiling demonstrated a decline in stearic acid and an increase in linolenic acid in acbp4 and acbp4acbp5 buds, respectively, over Col-0. Analysis of inflorescences from acbp4 and acbp5 revealed that there was an increase of AtACBP5 expression in acbp4, and an increase of AtACBP4 expression in acbp5. Deletion analysis of the AtACBP4 and AtACBP5 5′-flanking regions indicated the minimal promoter activity for AtACBP4 (−145/+103) and AtACBP5 (−181/+81). Electrophoretic mobility shift assays identified a pollen-specific cis-acting element POLLEN1 (AGAAA) mapped at AtACBP4 (−157/−153) which interacted with nuclear proteins from flower and this was substantiated by DNase I footprinting.

Abstract

In Arabidopsis thaliana, six acyl-CoA-binding proteins (ACBPs), designated as AtACBP1 to AtACBP6, have been identified to function in plant stress and development. AtACBP4 and AtACBP5 represent the two largest proteins in the AtACBP family. Despite having kelch-motifs and sharing a common cytosolic subcellular localization, AtACBP4 and AtACBP5 differ in spatial and temporal expression. Histological analysis on transgenic Arabidopsis expressing β-glucuronidase driven from the respective AtACBP4 and AtACBP5 promoters, as well as, qRT-PCR analysis revealed that AtACBP4 was expressed at stages 11–14 in mature pollen, while AtACBP5 was expressed at stages 7–10 in the microspores and tapetal cells. Immunoelectron microscopy using AtACBP4- or AtACBP5-specific antibodies further showed that AtACBP4 and AtACBP5 were localized in the cytoplasm. Chemical analysis of bud wax and cutin using gas chromatography-flame ionization detector and GC-mass spectrometry analyses revealed the accumulation of cuticular waxes and cutin monomers in acbp4, acbp5 and acbp4acbp5 buds, in comparison to the wild type. Analysis of inflorescences from acbp4 and acbp5 revealed that there was an increase of AtACBP5 expression in acbp4, and an increase of AtACBP4 expression in acbp5. Deletion analysis of the AtACBP4 and AtACBP5 5′-flanking regions indicated the minimal promoter region for AtACBP4 (−145/+103) and AtACBP5 (−181/+81). Electrophoretic mobility shift assays identified a pollen-specific cis-acting element POLLEN1 (AGAAA) within AtACBP4 (−157/−153) which interacted with nuclear proteins from flower and this was substantiated by DNase I footprinting. These results suggest that AtACBP4 and AtACBP5 both function in floral lipidic metabolism and they may play complementary roles in Arabidopsis microspore-to-pollen development.

Keywords

Acyl-CoA-binding protein Arabidopsis thaliana Lipid metabolism Pollen POLLEN1 Pollen tube 

Abbreviations

ACBP

Acyl-CoA-binding protein

DAG

Diacylglycerol

DFA

Dicarboxylic fatty acid

DGDG

Digalactosyldiacylglycerol

EMSAs

Electrophoretic mobility shift assays

FA

Fatty acid

FID

Flame ionization detector

GC-MS

Gas chromatography-mass spectrometry

GUS

β-Glucuronidase

HFA

Hydroxy fatty acid

MGDG

Monogalactosyldiacylglycerol

PC

Phosphatidylcholine

PE

Phosphatidylethanolamine

PI

Phosphatidylinositol

SEM

Scanning electron microscopy

TEM

Transmission electron microscopy

Supplementary material

11103_2016_557_MOESM1_ESM.docx (1.3 mb)
Supplementary material 1 (DOCX 1332 KB)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Zi-Wei Ye
    • 1
  • Jie Xu
    • 2
  • Jianxin Shi
    • 2
  • Dabing Zhang
    • 2
  • Mee-Len Chye
    • 1
  1. 1.School of Biological SciencesThe University of Hong KongHong KongChina
  2. 2.Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina

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