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Plant Molecular Biology

, Volume 90, Issue 6, pp 689–697 | Cite as

Differential growth responses of Brachypodium distachyon genotypes to inoculation with plant growth promoting rhizobacteria

  • Fernanda P. do Amaral
  • Vânia C. S. Pankievicz
  • Ana Carolina M. Arisi
  • Emanuel M. de Souza
  • Fabio Pedrosa
  • Gary StaceyEmail author
Article

Abstract

Plant growth promoting rhizobacteria (PGPR) can associate and enhance the growth of important crop grasses. However, in most cases, the molecular mechanisms responsible for growth promotion are not known. Such research could benefit by the adoption of a grass model species that showed a positive response to bacterial inoculation and was amenable to genetic and molecular research methods. In this work we inoculated different genotypes of the model grass Brachypodium distachyon with two, well-characterized PGPR bacteria, Azospirillum brasilense and Herbaspirillum seropedicae, and evaluated the growth response. Plants were grown in soil under no nitrogen or with low nitrogen (i.e., 0.5 mM KNO3). A variety of growth parameters (e.g., shoot height, root length, number of lateral roots, fresh and dry weight) were measured 35 days after inoculation. The data indicate that plant genotype plays a very important role in determining the plant response to PGPR inoculation. A positive growth response was observed with only four genotypes grown under no nitrogen and three genotypes tested under low nitrogen. However, in contrast, relatively good root colonization was seen with most genotypes, as measured by drop plate counting and direct, microscopic examination of roots. In particular, the endophytic bacteria H. seropedicae showed strong epiphytic and endophytic colonization of roots.

Keywords

Brachypodium distachyon Plant-beneficial bacteria interaction Azospirillum brasilense Herbaspirillum seropedicae 

Notes

Acknowledgments

Research was financially supported by the National Institute of Science and Technology-Biological Nitrogen Fixation, INCT-FBN, through the Brazilian Research Council—CNPq/MCT and the Cîencia Sem Fronteiras Program, Brazil (fellowships supporting F.P.A and. V.C S.P). Support was also provided by grants DE-FOA-0000223 and DESC0013978 (to G.S.) from the Department of Energy, Office of Biological and Environmental Research.

Author contribution

F.P.A. participated in this study, its design and coordination, performed the experiments, data collection, interpretation of the data and drafted the manuscript; V.C.S.P. participated in the experiment perform and data collection specially in the microscopy figures; A.C.M.A. participated in the design and coordination of the study; E.M.S. participated in the design and coordination of the study and performed the measurement; F.P. participated in the design and coordination of the study; G.S. conceived of the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.

Supplementary material

11103_2016_449_MOESM1_ESM.pdf (77 kb)
Supplementary material 1 (PDF 77 kb)
11103_2016_449_MOESM2_ESM.pdf (473 kb)
Supplementary material 2 (PDF 473 kb)

Supplementary material 3 (MOV 783 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Fernanda P. do Amaral
    • 1
  • Vânia C. S. Pankievicz
    • 2
  • Ana Carolina M. Arisi
    • 3
  • Emanuel M. de Souza
    • 2
  • Fabio Pedrosa
    • 2
  • Gary Stacey
    • 1
    Email author
  1. 1.Divisions of Plant Science and Biochemistry, C. S. Bond Life Science CenterUniversity of MissouriColumbiaUSA
  2. 2.Department of Biochemistry and Molecular BiologyFederal University of ParanáCuritibaBrazil
  3. 3.Department of Food Science and TechnologyFederal University of Santa CatarinaFlorianópolisBrazil

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