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Plant Molecular Biology

, Volume 86, Issue 3, pp 237–253 | Cite as

The CCCH zinc finger protein gene AtZFP1 improves salt resistance in Arabidopsis thaliana

  • Guoliang Han
  • Mingjie Wang
  • Fang Yuan
  • Na Sui
  • Jie Song
  • Baoshan Wang
Article

Abstract

The CCCH type zinc finger proteins are a super family involved in many aspects of plant growth and development. In this study, we investigated the response of one CCCH type zinc finger protein AtZFP1 (At2g25900) to salt stress in Arabidopsis. The expression of AtZFP1 was upregulated by salt stress. Compared to transgenic strains, the germination rate, emerging rate of cotyledons and root length of wild plants were significantly lower under NaCl treatments, while the inhibitory effect was significantly severe in T-DNA insertion mutant strains. At germination stage, it was mainly osmotic stress when treated with NaCl. Relative to wild plants, overexpression strains maintained a higher K+, K+/Na+, chlorophyll and proline content, and lower Na+ and MDA content. Quantitative real-time PCR analysis revealed that the expression of stress related marker genes KIN1, RD29B and RD22 increased more significantly in transgenic strains by salt stress. Overexpression of AtZFP1 also enhanced oxidative and osmotic stress tolerance which was determined by measuring the expression of a set of antioxidant genes, osmotic stress genes and ion transport protein genes such as SOS1, AtP5CS1 and AtGSTU5. Overall, our results suggest that overexpression of AtZFP1 enhanced salt tolerance by maintaining ionic balance and limiting oxidative and osmotic stress.

Keywords

AtZFP1 CCCH Zinc finger protein Salt stress Arabidopsis 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31300205, 31370420), Natural Science Research Foundation of Shandong (ZR2013CQ009) and the Program for Scientific research innovation team in Colleges and universities of Shandong Province.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Key Laboratory of Plant Stress Research, College of Life ScienceShandong Normal UniversityJinanChina

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