Plant Molecular Biology

, Volume 82, Issue 4–5, pp 393–416 | Cite as

Characterization of the GGPP synthase gene family in Arabidopsis thaliana

  • Gilles Beck
  • Diana Coman
  • Edgar Herren
  • M. Águila Ruiz-Sola
  • Manuel Rodríguez-Concepción
  • Wilhelm Gruissem
  • Eva Vranová


Geranylgeranyl diphosphate (GGPP) is a key precursor of various isoprenoids that have diverse functions in plant metabolism and development. The annotation of the Arabidopsis thaliana genome predicts 12 genes to encode geranylgeranyl diphosphate synthases (GGPPS). In this study we analyzed GGPPS activity as well as the subcellular localization and tissue-specific expression of the entire protein family in A. thaliana. GGPPS2 (At2g18620), GGPPS3 (At2g18640), GGPPS6 (At3g14530), GGPPS7 (At3g14550), GGPPS8 (At3g20160), GGPPS9 (At3g29430), GGPPS10 (At3g32040) and GGPPS11 (At4g36810) showed GGPPS activity in Escherichia coli, similar to activities reported earlier for GGPPS1 (At1g49530) and GGPPS4 (At2g23800) (Zhu et al. in Plant Cell Physiol 38(3):357–361, 1997a; Plant Mol Biol 35(3):331–341, b). GGPPS12 (At4g38460) did not produce GGPP in E. coli. Based on DNA sequence analysis we propose that GGPPS5 (At3g14510) is a pseudogene. GGPPS–GFP (green fluorescent protein) fusion proteins of the ten functional GGPP synthases localized to plastids, mitochondria and the endoplasmic reticulum, with the majority of the enzymes located in plastids. Gene expression analysis using quantitative real time-PCR, GGPPS promoter-GUS (β-glucuronidase) assays and publicly available microarray data revealed a differential spatio-temporal expression of GGPPS genes. The results suggest that plastids and mitochondria are key subcellular compartments for the synthesis of ubiquitous GGPP-derived isoprenoid species. GGPPS11 and GGPPS1 are the major isozymes responsible for their biosynthesis. All remaining paralogs, encoding six plastidial isozymes and two cytosolic isozymes, were expressed in specific tissues and/or at specific developmental stages, suggesting their role in developmentally regulated isoprenoid biosynthesis. Our results show that of the 12 predicted GGPPS encoded in the A. thaliana genome 10 are functional proteins that can synthesize GGPP. Their specific subcellular location and differential expression pattern suggest subfunctionalization in providing GGPP to specific tissues, developmental stages, or metabolic pathways.


Arabidopsis Isoprenoids Branchpoint Prenyl diphosphate synthase Geranylgeranyl diphosphate synthase 



Abscisic acid


Dimethylallyl diphosphate


Endoplasmic reticulum


Farnesyl diphosphate


Gibberellic acid


Green fluorescent protein


Geranylgeranyl diphosphate


Geranylgeranyl diphosphate synthase


Geranyl diphosphate




Isopentenyl diphosphate







This work was supported by a grant from ETH Zurich (TH-51 06-1) and the EU FP7 contract 245143 (TiMet). The Spanish Ministerio de Ciencia e Innovacion ( provided grants BIO2008-00432 and BIO2011-23680 to MRC and a doctoral FPI fellowship to ARS. We thank Biswapriya Biswavas Misra and Christian Barucker for their contribution to the work on subcellular localization. We thank Dr. Axel Schmidt for useful discussions on enzymatic activity assays.

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

11103_2013_70_MOESM1_ESM.pdf (232 kb)
Supplemental Table S1 as PDF Oligonucleotides used for GGPPSs amplification to construct pGEX-GGPPS (PDF 231 kb)
11103_2013_70_MOESM2_ESM.pdf (236 kb)
Supplemental Table S2 as PDF Oligonucleotides used for GGPPSs amplification to construct pENTR/D-TOPO-GGPPS-3` (PDF 235 kb)
11103_2013_70_MOESM3_ESM.pdf (249 kb)
Supplemental Table S3 as PDF Oligonucleotides used for RT-qPCR (PDF 248 kb)
11103_2013_70_MOESM4_ESM.pdf (35 kb)
Supplemental Table S4 as PDF Oligonucleotides used for GGPPSs promoter amplification to construct pENTR-D-TOPO-GGPPSpro (PDF 34 kb)
11103_2013_70_MOESM5_ESM.pdf (2.6 mb)
Supplemental Table S5 as PDF RT-qPCR and microarray expression of GGPPSs in seven Arabidopsis organs (PDF 2710 kb)
11103_2013_70_MOESM6_ESM.pdf (1.9 mb)
Supplemental Table S6 as PDF Microarray tissue-specific expression of the GGPPS genes (PDF 1986 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Gilles Beck
    • 1
  • Diana Coman
    • 1
  • Edgar Herren
    • 1
  • M. Águila Ruiz-Sola
    • 2
  • Manuel Rodríguez-Concepción
    • 2
  • Wilhelm Gruissem
    • 1
  • Eva Vranová
    • 1
    • 3
  1. 1.Department of Biology, Plant BiotechnologyETH ZurichZurichSwitzerland
  2. 2.Department of Molecular GeneticsCentre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UBBarcelonaSpain
  3. 3.Faculty of Science, Institute of Biology and EcologyP. J. Šafárik University KošiceKošiceSlovakia

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