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Plant Molecular Biology

, Volume 82, Issue 1–2, pp 147–154 | Cite as

Post-transcriptional control of light-harvesting genes expression under light stress

  • Maïna Floris
  • Roberto Bassi
  • Christophe RobagliaEmail author
  • Alessandro Alboresi
  • Elodie LanetEmail author
Article

Abstract

Plants have to deal with fluctuating light environment and the regulation of the photosynthetic apparatus is crucial for their survival. The large multigenic family of nuclear encoded chloroplastic proteins called light harvesting complex (LHC) is involved in both light harvesting and photoprotection. Changes in light intensity induce a complex set of molecular events within both the chloroplast and the cytoplasmic compartments of the cell leading to reorganization of the photosynthetic apparatus in order to optimize photosynthesis to the new conditions. In this study we have investigated the occurrence of translational regulations during light stress in Arabidopsis thaliana by using polysomes profiling. We have observed a strong effect of light on global translation activity of the cell. We show that individual LHC genes are translationally regulated in response to light conditions by changing the ratio between polysomal versus total messenger RNA. In addition, we found that cytoplasmic translational regulation can precede nuclear transcriptional regulation. Thus translational control appears as an important component of the crosstalk between chloroplast and the nucleus in plant cells.

Keywords

Translation LHC Light stress Polysomes Arabidopsis 

Notes

Acknowledgments

We are grateful to John C Gray for providing gun1-1 mutant. We also thank Tomas Morosinotto for discussions and comments. M.F. was supported by French Minister funding (MNRT). A.A. was supported by European Union Project 245070 FP7-KBBE-2009-3 SUNBIOPATH. E.L. was supported by Aix-Marseille University and EMBO short-term fellowship.

Supplementary material

11103_2013_46_MOESM1_ESM.pdf (253 kb)
Figure S1. Quantitative western blot analysis. Lhcb4 and Lhcb6 using thylakoids from control light and high light acclimated samples (treated by light during 15 days). Protein amount was normalized both to chlorophyll and CP47 (a core protein of PSII) quantity calculated by immunoblotting. The accumulation of Lhcb6 protein in plants treated by LL for 15 days is consistent with previously published data (Ballottari et al., 2007). (PDF 253 kb)
11103_2013_46_MOESM2_ESM.pdf (407 kb)
Figure S2. On the upper part are presented Et-Br stainings of RNA extracted from each 12 fractions of the sucrose gradient and separated on agarose gel. The position of rRNAs is indicated. On the lower panel are presented quantifications of 25S over total Et-Br signals that were measured on 10 fractions (fraction 1 and fraction 12 were excluded from the analysis). (PDF 407 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Laboratoire de Génétique et Biophysique des Plantes, Service de Biologie Végétale et de Microbiologie Environnementale, Unite Mixte de Recherche 7225, Commissariat à l’Energie Atomique, Centre National de la Recherche Scientifique, Faculte des Sciences de LuminyAix Marseille UniversiteMarseilleFrance
  2. 2.Dipartimento di BiotecnologieUniversità di VeronaVeronaItaly
  3. 3.IBDMLAix-Marseille University, CNRS, UMR 7288MarseilleFrance

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