OsSNDP1, a Sec14-nodulin domain-containing protein, plays a critical role in root hair elongation in rice
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Rice is cultivated in water-logged paddy lands. Thus, rice root hairs on the epidermal layers are exposed to a different redox status of nitrogen species, organic acids, and metal ions than root hairs growing in drained soil. To identify genes that play an important role in root hair growth, a forward genetics approach was used to screen for short-root-hair mutants. A short-root-hair mutant was identified and isolated by using map-based cloning and sequencing. The mutation arose from a single amino acid substitution of OsSNDP1 (Oryza sativa Sec14-nodulin domain protein), which shows high sequence homology with Arabidopsis COW1/AtSFH1 and encodes a phosphatidylinositol transfer protein (PITP). By performing complementation assays with Atsfh1 mutants, we demonstrated that OsSNDP1 is involved in growth of root hairs. Cryo-scanning electron microscopy was utilized to further characterize the effect of the Ossndp1 mutation on root hair morphology. Aberrant morphogenesis was detected in root hair elongation and maturation zones. Many root hairs were branched and showed irregular shapes due to bulged nodes. Many epidermal cells also produced dome-shaped root hairs, which indicated that root hair elongation ceased at an early stage. These studies showed that PITP-mediated phospholipid signaling and metabolism is critical for root hair elongation in rice.
KeywordsRoot hair Sec14-nodulin domain-containing protein (SNDP) Phosphatidylinositol transfer protein (PITP) Rice
This work was supported by grants from the Next-Generation BioGreen 21 Program (PJ008215 and PJ008168), the Rural Development Administration, Republic of Korea. Jingmiao Liu is supported by a scholarship from the BK21 program. We are grateful to Dr. Liam Dolan (University of Oxford, UK) for helping us with cryo-SEM work and Dr. Vytas Bankaitis (Texas A&M University, USA) for his constructive comments on our work.
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