Plant Molecular Biology

, Volume 82, Issue 1–2, pp 39–50 | Cite as

OsSNDP1, a Sec14-nodulin domain-containing protein, plays a critical role in root hair elongation in rice

  • Jin Huang
  • Chul Min Kim
  • Yuan-hu Xuan
  • Soon Ju Park
  • Hai Long Piao
  • Byoung Il Je
  • Jingmiao Liu
  • Tae Ho Kim
  • Bo-Kyeong Kim
  • Chang-Deok HanEmail author


Rice is cultivated in water-logged paddy lands. Thus, rice root hairs on the epidermal layers are exposed to a different redox status of nitrogen species, organic acids, and metal ions than root hairs growing in drained soil. To identify genes that play an important role in root hair growth, a forward genetics approach was used to screen for short-root-hair mutants. A short-root-hair mutant was identified and isolated by using map-based cloning and sequencing. The mutation arose from a single amino acid substitution of OsSNDP1 (Oryza sativa Sec14-nodulin domain protein), which shows high sequence homology with Arabidopsis COW1/AtSFH1 and encodes a phosphatidylinositol transfer protein (PITP). By performing complementation assays with Atsfh1 mutants, we demonstrated that OsSNDP1 is involved in growth of root hairs. Cryo-scanning electron microscopy was utilized to further characterize the effect of the Ossndp1 mutation on root hair morphology. Aberrant morphogenesis was detected in root hair elongation and maturation zones. Many root hairs were branched and showed irregular shapes due to bulged nodes. Many epidermal cells also produced dome-shaped root hairs, which indicated that root hair elongation ceased at an early stage. These studies showed that PITP-mediated phospholipid signaling and metabolism is critical for root hair elongation in rice.


Root hair Sec14-nodulin domain-containing protein (SNDP) Phosphatidylinositol transfer protein (PITP) Rice 



This work was supported by grants from the Next-Generation BioGreen 21 Program (PJ008215 and PJ008168), the Rural Development Administration, Republic of Korea. Jingmiao Liu is supported by a scholarship from the BK21 program. We are grateful to Dr. Liam Dolan (University of Oxford, UK) for helping us with cryo-SEM work and Dr. Vytas Bankaitis (Texas A&M University, USA) for his constructive comments on our work.

Supplementary material

11103_2013_33_MOESM1_ESM.pdf (94 kb)
Five-day-old seedlings from the OsSNDP1 wild type and mutant. OsSNDP1 wild type and mutant were grown in 1/2 MS for 5 days (PDF 95 kb)
11103_2013_33_MOESM2_ESM.pdf (420 kb)
Phylogenetic analysis and protein alignment of OsSNDP1, 2, 3 and COW1/AtSFH1. a Ten OsSNDP genes can be classified into three groups. Three genes from group I, including OsSNDP1 of Os10g03400, show the highest homology with COW1/AtSFH1 of Arabidopsis. b OsSNDP1, 2, and 3 protein sequences are aligned with Arabidopsis COW1. Sec14-like domains are framed with black rectangles. Nodulin-like domains are underlined with a solid line (PDF 420  kb)
11103_2013_33_MOESM3_ESM.pdf (186 kb)
The expression levels and root-hair lengths of 7 Atsfh1 lines transformed with wild type OsSNDP1 and 6 Atsfh1 lines transformed with mutant Ossndp1 cDNA. a The expression levels of OsSNDP1 and Ossndp1 in Atsfh1 mutants were measured by qRT-PCR using ubiquitin as an internal control. b Seven-day-old Arabidopsis seedlings from each complementation line were used to measure root hair lengths. CW denotes Atsfh1 complemented with wild type OsSNDP1 cDNA while CM denotes Atsfh1 with mutant Ossndp1 cDNA (PDF 187 kb)
11103_2013_33_MOESM4_ESM.pdf (92 kb)
Gene expression analysis of OsSNDP1 using the rice array database ( Expression patterns of OsSNDP1 in suspension cells, root, shoot, anther, stigma, ovary, embryo, endosperm, and 5-day seeds were examined using the rice Affymetrix expression database. The expression data were translated into the graph (PDF 92 kb)
11103_2013_33_MOESM5_ESM.pdf (177 kb)
Hydropathy analysis of OsSNDP1 and Ossndp1 proteins. Protein sequences of OsSNDP1 and Ossndp1 were analyzed by hydropathy analysis at using the Kyte& Doolittle method. a Hydropathy scores were compared between OsSNDP1 and Ossndp1 proteins. b Magnified area of box in a. Window size = 7 (PDF 178 kb)
11103_2013_33_MOESM6_ESM.pdf (169 kb)
The morphology and lengths of root-hairs and Ossndp1 expression levels of F1 Arabidopsis plants (Col/CM5 and Col/CM9) obtained from crosses between Columbia (Col) and two Ossndp1 Atsfh1 lines (CM5 and CM9; see Online Resource 3). a Root hairs of Columbia and F1 plants were inspected under a microscope. Bar = 500 μm. b Root-hair lengths of five F1 seedlings from the same cross were measured. c The expression levels of Ossndp1 in F1 seedlings were measured by qRT-PCR using ubiquitin as an internal control. All the seedlings were 7-days old (PDF 169 kb)
11103_2013_33_MOESM7_ESM.pdf (79 kb)
Primer sets for map-based cloning. Primer sets such as RM7545, RM7217, RM3152, S10026C, RM1650, and S10072 were obtained from public databases. The other primer sets were designed based on public rice sequence databases. Silver stain means that PCR products were separated on polyacrylamide gels and stained by a silver staining method. Stu I means PCR products were digested with Stu I (PDF 79 kb)
11103_2013_33_MOESM8_ESM.pdf (79 kb)
Primer sets for q-RT PCR. All the primer sets were performed under the condition of 95 °C 1 min (activation), 95 °C 10 s, 55 °C 10 s, 72 °C 15 s, and 40 cycles (PDF 79 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Jin Huang
    • 1
  • Chul Min Kim
    • 2
  • Yuan-hu Xuan
    • 1
  • Soon Ju Park
    • 1
  • Hai Long Piao
    • 1
  • Byoung Il Je
    • 1
  • Jingmiao Liu
    • 1
  • Tae Ho Kim
    • 3
  • Bo-Kyeong Kim
    • 4
  • Chang-Deok Han
    • 1
    Email author
  1. 1.Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC)Gyeongsang National UniversityJinjuKorea
  2. 2.Department of Plant SciencesUniversity of OxfordOxfordUK
  3. 3.Genomics Division, Department of Agricultural BiotechnologyNational Academy of Agricultural Science (NAAS), RDASuwonKorea
  4. 4.Rice Breeding and Cultivation Division, Department of Rice and Winter Cereal Crop (NICS)RDAIksanKorea

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