Proteome changes in wild and modern wheat leaves upon drought stress by two-dimensional electrophoresis and nanoLC-ESI–MS/MS
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To elucidate differentially expressed proteins and to further understand post-translational modifications of transcripts, full leaf proteome profiles of two wild emmer (Triticum turgidum ssp. dicoccoides TR39477 and TTD22) and one modern durum wheat (Triticum turgidum ssp. durum cv. Kızıltan) genotypes were compared upon 9-day drought stress using two-dimensional gel electrophoresis and nano-scale liquid chromatographic electrospray ionization tandem mass spectrometry methods. The three genotypes compared exhibit distinctive physiological responses to drought as previously shown by our group. Results demonstrated that many of the proteins were common in both wild emmer and modern wheat proteomes; of which, 75 were detected as differentially expressed proteins. Several proteins identified in all proteomes exhibited drought regulated patterns of expression. A number of proteins were observed with higher expression levels in response to drought in wild genotypes compared to their modern relative. Eleven protein spots with low peptide matches were identified as candidate unique drought responsive proteins. Of the differentially expressed proteins, four were selected and further analyzed by quantitative real-time PCR at the transcriptome level to compare with the proteomic data. The present study provides protein level differences in response to drought in modern and wild genotypes of wheat that may account for the differences of the overall responses of these genotypes to drought. Such comparative proteomics analyses may aid in the better understanding of complex drought response and may suggest candidate genes for molecular breeding studies to improve tolerance against drought stress and, thus, to enhance yields.
KeywordsDrought stress Modern wheat Wild emmer nanoLC-ESI–MS/MS Proteomics 2-DE
Authors acknowledge TUBITAK for the financial support. We would like to thank to Dr. Megan Bowman for reviewing the manuscript.
Conflict of interest
The authors declare that they have no conflict of interest.
- Bhullar NK, Street K, Mackay M, Yahiaoui N, Keller B (2009) Unlocking wheat genetic resources for the molecular identification of previously undescribed functional alleles at the Pm3 resistance locus. Proc Natl Acad Sci USA 106(23):9519–9524. doi: 10.1073/pnas.0904152106 PubMedCrossRefGoogle Scholar
- Caruso G, Cavaliere C, Guarino C, Gubbiotti R, Foglia P, Lagana A (2008) Identification of changes in Triticum durum L. leaf proteome in response to salt stress by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. Anal Bioanal Chem 391(1):381–390. doi: 10.1007/s00216-008-2008-x Google Scholar
- Chantret N, Salse J, Sabot F, Rahman S, Bellec A, Laubin B, Dubois I, Dossat C, Sourdille P, Joudrier P, Gautier MF, Cattolico L, Beckert M, Aubourg S, Weissenbach J, Caboche M, Bernard M, Leroy P, Chalhoub B (2005) Molecular basis of evolutionary events that shaped the hardness locus in diploid and polyploid wheat species (Triticum and Aegilops). Plant Cell 17(4):1033–1045. doi: 10.1105/tpc.104.029181 PubMedCrossRefGoogle Scholar
- Fan P, Feng J, Jiang P, Chen X, Bao H, Nie L, Jiang D, Lv S, Kuang T, Li Y (2011) Coordination of carbon fixation and nitrogen metabolism in Salicornia europaea under salinity: comparative proteomic analysis on chloroplast proteins. Proteomics 11(22):4346–4367. doi: 10.1002/pmic.201100054 PubMedCrossRefGoogle Scholar
- Galle A, Csiszar J, Secenji M, Guoth A, Cseuz L, Tari I, Gyorgyey J, Erdei L (2009) Glutathione transferase activity and expression patterns during grain filling in flag leaves of wheat genotypes differing in drought tolerance: response to water deficit. J Plant Physiol 166(17):1878–1891. doi: 10.1016/j.jplph.2009.05.016 PubMedCrossRefGoogle Scholar
- Petrov VD, Van Breusegem F (2012) Hydrogen peroxide-a central hub for information flow in plant cells. AoB Plants 2012:pls014. doi: 10.1093/aobpla/pls014
- Ravanel S, Block MA, Rippert P, Jabrin S, Curien G, Rebeille F, Douce R (2004) Methionine metabolism in plants: chloroplasts are autonomous for de novo methionine synthesis and can import S-adenosylmethionine from the cytosol. J Biol Chem 279(21):22548–22557. doi: 10.1074/jbc.M313250200 PubMedCrossRefGoogle Scholar
- Salekdeh GH, Siopongco J, Wade LJ, Ghareyazie B, Bennett J (2002) Proteomic analysis of rice leaves during drought stress and recovery. Proteomics 2(9):1131–1145. doi: 10.1002/1615-9861(200209)2:9<1131:AID-PROT1131>3.0.CO;2-1 PubMedCrossRefGoogle Scholar
- Shin KH, Kamal AHM, Cho K, Choi JS, Jin Y, Paek NC, Lee YW, Lee JK, Park JC, Kim HT, Heo HY, Woo SH (2011) Defense proteins are induced in wheat spikes exposed to Fusarium graminearum. Plant Omics J 4(5):270–277Google Scholar