Analysis of the chloroplast proteome in arc mutants and identification of novel protein components associated with FtsZ2
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Chloroplasts are descendants of cyanobacteria and divide by binary fission. The number of chloroplasts is regulated in a cell type-specific manner to ensure that specialized cell types can perform their functions optimally. Several protein components of the chloroplast division apparatus have been identified in the past several years, but how this process is regulated in response to developmental status, environmental signals and stress is still unknown. To begin to address this we undertook a proteomic analysis of three accumulation and replication of chloroplasts mutants that show a spectrum of plastid division perturbations. We show that defects in the chloroplast division process results in changes in the abundance of proteins when compared to wild type, but that the profile of the native stromal and membrane complexes remains unchanged. Furthermore, by combining BN-PAGE with protein interaction assays we show that AtFtsZ2-1 and AtFtsZ2-2 assemble together with rpl12A and EF-Tu into a novel chloroplast membrane complex.
Keywords2D-DIGE BN-PAGE FtsZ Chloroplast division LC–MS/MS
We thank Astrid Elisabeth Tveitaskog for assistance with 2D-PAGE and chloroplast isolation. This work was supported by a FUGE Norwegian Research Council grant to SGM.
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