Plant Molecular Biology

, Volume 80, Issue 3, pp 241–253 | Cite as

OsWRKY30 is activated by MAP kinases to confer drought tolerance in rice

  • Huaishun Shen
  • Citao Liu
  • Yi Zhang
  • Xiuping Meng
  • Xin Zhou
  • Chengcai Chu
  • Xiping Wang
Article

Abstract

Both the WRKY transcription factor (TF) and MAP kinases have been shown to regulate gene expression in response to biotic and abiotic stresses in plants. Several reports have shown that WRKY TFs may function downstream of MAPK cascades. Here, we have shown that OsWRKY30 interacted with OsMPK3, OsMPK4, OsMPK7, OsMPK14, OsMPK20-4, and OsMPK20-5, and could be phosphorylated by OsMPK3, OsMPK7, and OsMPK14. Overexpression of OsWRKY30 in rice dramatically increased drought tolerance. Overexpression of OsWRKY30AA, in which all SP (serine residue followed by proline residue) sites were replaced by AP (A, alanine), resulted in no improvement in drought tolerance. In addition, the function of transcriptional activation of OsWRKY30 was impaired after SP was replaced by AP. These results proved that the phosphorylation of OsWRKY30 by MAPKs was crucial in order for OsWRKY30 to perform its biological function.

Keywords

WRKY MAPK Drought tolerance Phosphorylation Transcriptional activation 

Supplementary material

11103_2012_9941_MOESM1_ESM.doc (572 kb)
Supplementary material 1 (DOC 572 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Huaishun Shen
    • 1
    • 2
  • Citao Liu
    • 2
    • 3
  • Yi Zhang
    • 2
  • Xiuping Meng
    • 2
  • Xin Zhou
    • 1
  • Chengcai Chu
    • 3
  • Xiping Wang
    • 2
  1. 1.Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxiPeople’s Republic of China
  2. 2.National Engineering Research Center for Molecular Crop DesignBeijing Weiming Kaituo Agriculture Biotech Co., LtdBeijingPeople’s Republic of China
  3. 3.State Key Laboratory of Plant Genomics and Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingPeople’s Republic of China

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