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Plant Molecular Biology

, Volume 78, Issue 4–5, pp 351–359 | Cite as

TH1, a DUF640 domain-like gene controls lemma and palea development in rice

  • Xiaojiao Li
  • Lianjun Sun
  • Lubin Tan
  • Fengxia Liu
  • Zuofeng Zhu
  • Yongcai Fu
  • Xianyou Sun
  • Xuewen Sun
  • Daoxin Xie
  • Chuanqing SunEmail author
Article

Abstract

The developmental regulation of grasses lemma and palea and their relationship to the floral organs in dicots had been variously explicated and extensively debated. Here, we characterized a triangular hull mutant th1-1 from EMS-mutagenized Oryza sativa ssp. indica cv. 93-11. The th1-1 mutant exhibited obviously triangular hull with tortuous and slender lemma/palea. Using a map-based cloning strategy, the TH1 gene was narrowed down to a 60-kb region on the long arm of chromosome 2. Sequence verification revealed that the th1-1 mutant harbored 1-bp deletion in exon 2 of LOC_Os02g56610 which resulted in a frame-shift mutation. The RNA-interference transgenic plants of LOC_Os02g56610 displayed a similar phenotype to the th1 mutant. Consequently, LOC_Os02g56610 was identified as the TH1 gene which encoded 248 amino acids and contained a DUF640 domain. RT-PCR analysis and GUS staining showed that the transcripts of TH1 mainly accumulated in young inflorescence, lemma and palea of spikelet. These results suggested that TH1 was an important gene controlling the lemma and palea development in rice.

Keywords

Triangular hull Lemma Palea TH1 Rice (Oryza sativa

Notes

Acknowledgments

We thank Prof. Zhizhong Gong for his kindest help. This work was supported by Self-Regulated Projects of State Key Laboratory of Plant Physiology and Biochemistry, and by grants from the National Program of Transgenic Variety Development (2008ZX08009-004, 2008ZX08009-008).

Supplementary material

11103_2011_9868_MOESM1_ESM.doc (36 kb)
Supplementary material 1 (DOC 36 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Xiaojiao Li
    • 1
  • Lianjun Sun
    • 1
  • Lubin Tan
    • 1
  • Fengxia Liu
    • 1
  • Zuofeng Zhu
    • 1
  • Yongcai Fu
    • 1
  • Xianyou Sun
    • 1
  • Xuewen Sun
    • 1
  • Daoxin Xie
    • 2
  • Chuanqing Sun
    • 1
    Email author
  1. 1.State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Genetics and BreedingChina Agricultural UniversityBeijingChina
  2. 2.School of Life SciencesTsinghua UniversityBeijingChina

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